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New Tools for Small RNA Analysis

d as they do not require gel purification. Ambion has developed and optimized kits for both probe-labeling methods -- the mirVana miRNA Probe Construction Kit for IVT and the mirVana Probe & Marker Kit for 5' end labeling.

Figure 4. Comparison Between In Vitro Transcribed (IVT) and 5' End Labeled Probes. The expression of miR-16 miRNA and 5S rRNA was analyzed in 0.5, 1, 2 or 4 g of FirstChoice Total RNA from mouse kidney included in the mirVana miRNA Detection Kit using the indicated DNA or RNA probes included in the mirVana miRNA Probe Construction Kit (IVT probes) or mirVana Probe & Marker Kit (5' end labeled probes).


The mirVana miRNA Probe Construction Kit includes reagents for preparation of IVT templates as well as reagents for the IVT reaction. All you need to provide is an inexpensive, desalted, target-specific DNA oligonucleotide. A short sequence cleavable by RNase A and T1 is added to the 5' end of probe during the procedure. Probes prepared with the kit are therefore suitable not only for solution hybridization assays, but also for Northern blot or in situ hybridization analysis (using nonisotopically labeled probes).

The new mirVana Probe & Marker Kit provides reagents for the rapid preparation and purification of short, high specific activity 5' end-labeled RNA probes. Such probes are widely used in a variety of assays, including RNA structure/function studies, Northern blot and primer extension analysis. For solution hybridization assays, the antisense
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