Navigation Links
New Tools for Small RNA Analysis

of miR-124, previously determined by Northern blot analysis using 30 g of total RNA (3). In contrast, only 1 g of total RNA was used in the solution hybridization assay. Moreover, miR-124 expression could be detected in as little as 10 ng of total brain RNA after overnight exposure of the gel (data not shown).

Figure 3. Tissue-specific expression of miR-124 miRNA. miR-124 was detected in 1 g of the indicated FirstChoice Total RNA from 14 different human tissues using the mirVana miRNA Detection Kit and a 5' end-labeled RNA probe prepared with the mirVana Probe & Marker kit. Gel was exposed for 5 hours.


Another application of the mirVana miRNA Detection Kit, is the detection of two or more RNAs in the same sample. For instance, an expressed siRNA and its target mRNA can be monitored simultaneously. See Application: Analysis of miRNA Recovery After Gel Purification (sidebar) or the Ambion miRNA Resource for additional applications of this versatile kit.


Preparation of RNA Probes
Small RNA probes for the solution hybridization assay can be readily prepared using one of two different methods: in vitro transcription (IVT) or 5' end labeling of an existing RNA oligonucleotide. Depending on the probe sequence and the radiolabeled nucleotide used, IVT probes typically result in ~2-fold greater sensitivity than 5' end labeled probes due to their higher specific activity (Figure 4). In contrast, 5' labeled RNA probes are more easily generate
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells
2. Tools for Proteomic Science II
3. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
4. Research Tools for Analysis of ZAP-70
5. SNPs Powerful Tools for Association Studies
6. New Tools for Making and Processing Protein Microarrays.
7. Fast Isolation of Total RNA from Small Numbers of Cells
8. Detecting Attomole Amounts of Small RNA
9. Got Small RNA?
10. Rapidly Prepare Probes and Markers for Small RNA Analysis
11. Ultra-Fast Purification of Small Nucleic Acids
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New Tools for Small RNA Analysis

(Date:9/18/2014)... , Sept. 18, 2014 About POCT ... is performed outside a clinical laboratory. It helps ... administrating appropriate treatments and medication. POCT is gaining ... of lifestyle diseases such as diabetes, heart disease, ... POCT devices are standardized to minimize errors during ...
(Date:9/18/2014)... September 18, 2014 Texas Governor ... Sharp, Texas A&M Health Science Center CEO Brett ... of Health and Human Services (HHS), State of ... national pandemic influenza vaccine manufacturing facility in Bryan, ... anchor for the Texas A&M Biocorridor – a ...
(Date:9/18/2014)... , Sept. 18, 2014 Research and ... "Global Companion Diagnostics (Polymerase Chain Reaction, In-situ hybridization, Immunohistochemistry) ... to their offering. The global companion ... of 22.7% from 2014 to 2019. Factors such as ... adoption of companion diagnostics by the pharmaceutical industry, support ...
(Date:9/18/2014)... SEATTLE , Sept. 18, 2014 /PRNewswire/ - Oncothyreon ... pricing of previously announced concurrent but separate underwritten offerings ... price to the public of $2.00 per share, for ... of its Series A Convertible Preferred Stock at a ... expected gross proceeds of $20 million. Each share of ...
Breaking Biology Technology:POCT Market in China 2014-2018 2Texas A&M Dedicates National Pandemic Influenza Vaccine Manufacturing Facility, on Track for 2016 Start-Up Phase 2Texas A&M Dedicates National Pandemic Influenza Vaccine Manufacturing Facility, on Track for 2016 Start-Up Phase 3Texas A&M Dedicates National Pandemic Influenza Vaccine Manufacturing Facility, on Track for 2016 Start-Up Phase 4Global Companion Diagnostics (Polymerase Chain Reaction, In-situ hybridization, Immunohistochemistry) Market - Trends & Forecasts to 2019 2Global Companion Diagnostics (Polymerase Chain Reaction, In-situ hybridization, Immunohistochemistry) Market - Trends & Forecasts to 2019 3Oncothyreon Prices $40 Million Concurrent Public Offerings of Common Stock and Preferred Stock 2Oncothyreon Prices $40 Million Concurrent Public Offerings of Common Stock and Preferred Stock 3
... Biotechnology companies are building on what they ... decade and are expected to drive this market ... Genetic Engineering and Biotechnology News ( GEN ... number of advantages, including reducing reagent consumption, increasing ...
... Mach One Corporation,(Pink Sheets: MNCN), a biological solutions company ... to the agricultural and consumer,market, announced today that it ... an Investor. , Mach One ... for,its first production facility in Wisconsin. The Original ...
... ABAX ), a medical products company manufacturing point-of-care ... its financial results for the third quarter of fiscal year ... 4:15 p.m. ET on Thursday, January 29, 2009.Participants can dial ... or can listen via a live Internet web cast, which ...
Cached Biology Technology:GEN Reports on Growing Reliance on Microfluidics Technology 2Mach One Completes Note Exchange 2Abaxis to Report Third Quarter Fiscal 2009 Financial Results Thursday, January 29, 2009 2
(Date:9/18/2014)... matter how many times it,s demonstrated, it,s still ... , But by using a signaling system ... their behavior to suit their population. In short, ... present, and act accordingly. , Once the population ... change from innocuous to pathogenic, or from unhelpful ...
(Date:9/18/2014)... information is stored in DNA, tiny strands of nucleic ... bodies. To express this genetic data, our DNA is ... into proteins that perform tasks in our cells. ... of RNA molecule. Unlike all other known RNAs, this ... circRNA molecules are abundant, little has been known about ...
(Date:9/18/2014)... 2014Many Americans across racial and ethnic groups describe ... on their day-to-day life, more so than other ... speech (57% of African-Americans, 49% of non-Hispanic whites, ... asked which disease or ailment is the worst ... among African-Americans followed by AIDS/HIV. Hispanics and ...
Breaking Biology News(10 mins):Researchers study vital 'on/off switches' that control when bacteria turn deadly 2Researchers study vital 'on/off switches' that control when bacteria turn deadly 3Scientists find how mysterious 'circular RNA' is formed, claim muscular dystrophy link 2Americans rate losing eyesight as having greatest impact on their lives 2Americans rate losing eyesight as having greatest impact on their lives 3
... heart-healthy, essential omega-3 fatty acid is about to improve ... advance will move biotechnology onto the average consumer,s daily ... deliver positive environmental impacts and increase production to feed ... on Horizon , Recently, the U.S. Food and Drug ...
... COLLEGE STATION "Four and 20 black birds baked in ... discovery, backed by DNA analysis, means scientists now know there is ... Africa than was previously thought. And if Dr. Gary Voelker has ... its susceptibility to the deforestation now occurring across its native habitat. ...
... two different protection programs to safeguard them from getting ... stopping and developing cancer. Until now, researchers assumed that ... Now for the first time, using an animal model ... (MDC) Berlin-Buch and the Charit University Hospital Berlin ...
Cached Biology News:New biotech advance to add heart healthy omega-3s to US diet 2African bird discovery proves there is something new under the sun 22 at 1 stroke -- how cells protect themselves from cancer 2
AADAC Immunogen: AADAC (NP_001077, 201 a.a. ~ 301 a.a) partial recombinant protein with GST tag. Storage: -20 C, Avoid Freeze/Thaw Cycles *Available for distribution in the US only...
Mouse monoclonal [CIa] to Ia Antigen (Biotin) ( Abpromise for all tested applications)....
Goat polyclonal to FBXO2 ( Abpromise for all tested applications). entrezGeneID: 26232 SwissProtID: Q9UK22...
Phospho-TAK1 (Thr184/187) (90C7) Rabbit mAb Shipping Temperature: Cold Storage Temperature: -20C...
Biology Products: