Navigation Links
New Tools for Small RNA Analysis

p://www.ambion.com/prod/fcrna">"miRNA certified" FirstChoice RNAs.


Sensitive Detection of Small RNAs
Although Northern analysis can be used to detect and quantitate small RNA, the technique is labor-intensive and, more importantly, not very sensitive. Ambion scientists have developed a sensitive assay that has been optimized for small RNA quantitation--the patented mirVana miRNA Detection Kit--which is based on solution hybridization and subsequent RNase protection. Short radiolabeled RNA probe is simply mixed with total RNA in an optimized hybridization buffer and all single-stranded RNAs (e.g. unhybridized probe, mRNAs, rRNA) are then digested by a mixture of RNase A and T1. Probe:target RNA hybrids are quickly recovered using a patented single-step RNase inactivation/precipitation procedure, run on a denaturing polyacrylamide gel, and visualized by autoradiography. In this assay, full-length and protected probes are easily distinguished by the presence of a short sequence at the 5' or 3' end of probes that is not complementary to the RNA target (Figure 2).

Figure 2. The mirVana miRNA Detection Kit for small RNA Detection


The mirVana miRNA Detection Kit has been used to detect miRNAs and siRNAs in a variety of tissues and cells (see poster for more information). The sensitivity and specificity of the assay is illustrated in Figure 3 where miR-124 miRNA was detected only in human brain total RNA. This experiment confirms the tissue-specific expression
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells
2. Tools for Proteomic Science II
3. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
4. Research Tools for Analysis of ZAP-70
5. SNPs Powerful Tools for Association Studies
6. New Tools for Making and Processing Protein Microarrays.
7. Fast Isolation of Total RNA from Small Numbers of Cells
8. Detecting Attomole Amounts of Small RNA
9. Got Small RNA?
10. Rapidly Prepare Probes and Markers for Small RNA Analysis
11. Ultra-Fast Purification of Small Nucleic Acids
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New Tools for Small RNA Analysis

(Date:9/23/2014)... The Minnesota High Tech Association ... cellular modems , platforms and application-specific remote monitoring ... for the 2014 Tekne Awards. The awards ... Convention Center on Thursday, November 13th. The Tekne ... shown superior technology innovation and leadership. , ...
(Date:9/22/2014)... Boston, MA (PRWEB) September 22, 2014 ... major scientific breakthrough in drought tolerant grass seed this ... generation grass seed. The announcement coincides with the fact ... in the Spring as many believe. , As ... 300 rave and favorable reviews by industry experts and ...
(Date:9/22/2014)... LEGO-like components developed by researchers at the USC ... to build a 3-D microfluidic system quickly and ... hand. , Microfluidic systems are used in ... precisely manipulate small volumes of fluids for use ... pathogen detection, clinical diagnostic testing, and synthetic chemistry. ...
(Date:9/22/2014)... have generated a new star-shaped molecule made up of interlocking ... created. , Known as a ,Star of David, ... a quarter of a century and the team,s findings are ... Consisting of two molecular triangles, entwined about each other three ... each triangle is 114 atoms in length around the ...
Breaking Biology Technology:NimbeLink Named Finalist for 15th Annual Tekne Awards 2NimbeLink Named Finalist for 15th Annual Tekne Awards 3Pearl's Premium Ultra Low Maintenance Lawn Seed Announces A Major Breakthrough In Drought Tolerant Grass Seed 2Lego-like modular components make building 3-D 'labs-on-a-chip' a snap 2Lego-like modular components make building 3-D 'labs-on-a-chip' a snap 3Lego-like modular components make building 3-D 'labs-on-a-chip' a snap 4Lego-like modular components make building 3-D 'labs-on-a-chip' a snap 5
... to Success, EL SEGUNDO, Calif., Oct. 3 ... characteristics that position them for,competitive advantage, according to ... from Computer Sciences Corporation (NYSE: CSC ),Supply ... The,survey, completed by supply chain professionals in 21 ...
... Cell,Genesys, Inc. (Nasdaq: CEGE ), today announced that ... present a company update on,Wednesday, October 10, at 10:15 ... San Francisco, California. A live audio webcast of ... of the Cell Genesys website, http://www.cellgenesys.com . If you ...
... a privately held,medical device firm, announced that it has ... the Crux IVC Filter. The filter, used to,prevent an ... Medical Center by David Rosenthal, Chief of Vascular Surgery. ... a PE and,between 120,000 - 150,000 die as a ...
Cached Biology Technology:2007 Global Survey Identifies Characteristics of Supply Chain Leaders 22007 Global Survey Identifies Characteristics of Supply Chain Leaders 32007 Global Survey Identifies Characteristics of Supply Chain Leaders 4Cell Genesys to Present at the BIO Investor Forum 2Crux Biomedical Announces First Successful U.S. Implant of Its New Retrievable Inferior Vena Cava (IVC) Filter 2
(Date:9/21/2014)... such as mussels and barnacles secrete very sticky proteins ... even underwater. Inspired by these natural adhesives, a team ... be used to repair ships or help heal wounds ... adhesives, the MIT researchers engineered bacteria to produce a ... well as a bacterial protein found in biofilms ...
(Date:9/19/2014)... the unique bioelectric signaling system of the electric fish, ... The OU researcher is working to understand how these ... used to map the world around them. Electric ... discharges a second throughout their lives. The energetic ... necessary for survival. , "There is evidence that electric ...
(Date:9/19/2014)... Inc. (Nasdaq: NXTD and NXTDW) ("Nxt-ID" or the ... updates the "Wocket in Your Pocket" celebrity ad campaign, featuring former ... classes, Vinny Pazienza (PAZ). , ... actor Ciaran Hinds and Sons of Anarchy ... have been cast in Bleed for This . ...
Breaking Biology News(10 mins):Engineered proteins stick like glue -- even in water 2Engineered proteins stick like glue -- even in water 3NXT-ID's Vinny Paz Celebrity Ad Wocket Update: Katey Sagal and Ciaran Hinds Attached to His Upcoming Movie 2NXT-ID's Vinny Paz Celebrity Ad Wocket Update: Katey Sagal and Ciaran Hinds Attached to His Upcoming Movie 3
... Had a bad day? Extending your normal exercise routine ... to Penn State researchers, who found that people,s satisfaction ... more than usual. "We found that people,s satisfaction ... activity," said Jaclyn Maher, graduate student in kinesiology. "The ...
... A new University of Michigan study shows that when ... likelihood of forming new collaborations and obtaining funding increases ... well., "Our analyses clearly show that there are benefits ... sociology and organizational studies. Researchers who occupy the ...
... release is available in German . ... out that the chemical triclosan is one of those ... that are still not sufficiently monitored. With extensive monitoring ... comprehensive than standard monitoring procedures, concentrations of the chemical ...
Cached Biology News:Exercise boosts satisfaction with life, researchers find 2Sharing space: 2Sharing space: 3Triclosan needs to be monitored 2
Mouse monoclonal [7A6] to NFAT2 ( Abpromise for all tested applications). entrezGeneID: 4772 SwissProtID: O95644...
Request Info...
Immunogen: C terminus [VQLKRSKNDSKPYC] of cardiac elav-type RNA-binding protein (ETR-3) Storage: -20 C, Avoid Freeze/Thaw Cycles...
Standard 2" and 3" Fiberboard Boxes can be sold separately or with 16, 25, 36, 49, 64, 81 or 100 cell dividers. Fiberboard boxes can also be ordered with corresponding freezer racks...
Biology Products: