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New Tools for Small RNA Analysis

P>Figure 1. A Fully Integrated System for Small RNA Analysis.


Isolation of Small RNAs
There are two widely used methods for isolating total RNAs: 1) phenol/chaotropic reagent extraction, and 2) binding to and elution from glass fiber filters (GFF). Ambion has determined that many small RNAs are not retained when total RNA is extracted by traditional GFF methods which are commonly used in most laboratories (see poster for more information). We have, however, developed a modified GFF-based procedure--through the use of optimized binding and wash solutions--that provides quantitative yield of miRNAs and other small RNAs from virtually any tissue or cell line. A modification of this procedure can also be used to enrich small RNAs from a total RNA population. This technology is now available as the mirVana miRNA Isolation Kit (patent pending).


"miRNA Certified" FirstChoice RNAs
When purchasing commercially available purified RNAs, it is also important to consider the RNA isolation method used to isolate the RNA. Most of Ambion's FirstChoice Total RNAs have been validated for the presence of small RNA molecules--and we are constantly working on optimized isolation procedures for these and other tissue and cell line samples. Visit our web catalog for an up-to-date list of

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