Navigation Links
New Tools for Small RNA Analysis

P>Figure 1. A Fully Integrated System for Small RNA Analysis.


Isolation of Small RNAs
There are two widely used methods for isolating total RNAs: 1) phenol/chaotropic reagent extraction, and 2) binding to and elution from glass fiber filters (GFF). Ambion has determined that many small RNAs are not retained when total RNA is extracted by traditional GFF methods which are commonly used in most laboratories (see poster for more information). We have, however, developed a modified GFF-based procedure--through the use of optimized binding and wash solutions--that provides quantitative yield of miRNAs and other small RNAs from virtually any tissue or cell line. A modification of this procedure can also be used to enrich small RNAs from a total RNA population. This technology is now available as the mirVana miRNA Isolation Kit (patent pending).


"miRNA Certified" FirstChoice RNAs
When purchasing commercially available purified RNAs, it is also important to consider the RNA isolation method used to isolate the RNA. Most of Ambion's FirstChoice Total RNAs have been validated for the presence of small RNA molecules--and we are constantly working on optimized isolation procedures for these and other tissue and cell line samples. Visit our web catalog for an up-to-date list of

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells
2. Tools for Proteomic Science II
3. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
4. Research Tools for Analysis of ZAP-70
5. SNPs Powerful Tools for Association Studies
6. New Tools for Making and Processing Protein Microarrays.
7. Fast Isolation of Total RNA from Small Numbers of Cells
8. Detecting Attomole Amounts of Small RNA
9. Got Small RNA?
10. Rapidly Prepare Probes and Markers for Small RNA Analysis
11. Ultra-Fast Purification of Small Nucleic Acids
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New Tools for Small RNA Analysis

(Date:9/2/2014)... University of Oregon chemist Geraldine Richmond, have tapped ... synthetic proteins called peptoid nanosheets that mimic complex ... detailed this week in a paper placed online ... the National Academy of Sciences -- is ... two-dimensional peptoid nanosheets that can be used in ...
(Date:9/2/2014)... , Sept. 2, 2014  TransCelerate BioPharma ... a recommended approach for protecting personal data in ... researchers, patients and others. The document describes an ... information from CSRs and other related clinical trial ... The goal of the TransCelerate CSR Redaction ...
(Date:9/2/2014)... 02, 2014 Opertech Bio, Inc., ... efficient, cost effective approach to sensory evaluation, today ... by the U.S. Patent and Trademark Office, encompassing ... , “Opertech’s taste evaluation technology called Microtiter ... high throughput system for rapid characterization of taste ...
(Date:9/2/2014)... 2014 /PRNewswire/ - Oncothyreon Inc. (Nasdaq: ONTY ) announced ... Executive Officer, will present at the Rodman & Renshaw 16 ... York on Tuesday, Sept. 9, 2014 at 2:05 ... of the presentation will be accessible by visiting the Oncothyreon ... About Oncothyreon Oncothyreon is ...
Breaking Biology Technology:UO-Berkeley Lab unveil new nano-sized synthetic scaffolding technique 2TransCelerate BioPharma Inc. Releases Recommended Approach for Protecting Personal Data in Clinical Study Reports 2TransCelerate BioPharma Inc. Releases Recommended Approach for Protecting Personal Data in Clinical Study Reports 3Opertech Bio Receives U.S. Patent for High-Throughput Taste Evaluation Technology 2
... drug discovery and development services, cell lines, and reagents ... Feb. 26 The leading experts in ion channels ... discovery, development, and safety - are now united. ChanTest, ... validated cell lines, announced that it has acquired Rockville, ...
... the availability of Trade Alerts on stocks making news today. ... free by visiting: http://www.BeaconEquity.com/m Today,s Trade Alerts include: ... Industries Ltd. (Nasdaq: TEVA ), Allergan Inc. (NYSE: ... ), Vertex Pharmaceuticals Inc. (Nasdaq: VRTX ) and ...
... announced today that it has received U.S. Food and ... using the Biomerix Biomaterial(TM), REVIVE acts as a tissue ... in a variety of soft tissue repair procedures, including ... procedures per year, inguinal hernia repair is one of ...
Cached Biology Technology:ChanTest Acquires Applied Cell Sciences 2ChanTest Acquires Applied Cell Sciences 3ChanTest Acquires Applied Cell Sciences 4Beacon Equity Issues Technical Trade Alerts on Drug Manufacturer Stocks: FRX, TEVA, AGN, CEPH, VRTX, ADLR 2Biomerix Corporation Announces FDA Clearance and Market Launch of REVIVE(TM) for Use in Soft Tissue Repair Procedures 2
(Date:9/1/2014)... television making us fat? An increasing amount of research ... consumption and a more sedentary lifestyle. Now, a new ... is alike. Some TV programs might lead people to ... that if you,re watching an action movie while snacking ... Tal, Ph.D. lead author on the new article just ...
(Date:9/1/2014)... Conventional wisdom has long held that corals ... reefs are passive organisms that rely entirely ... as nutrients and oxygen. But now scientists at ... in Israel have found that they are far ... into turbulent patterns that greatly enhance their ability ...
(Date:9/1/2014)... basic building block of DNAcould initiate fragile X syndrome, the most ... The Journal of Cell Biology . , ... on the X chromosome called fragile X mental retardation 1 ... men carry a so-called premutation, in which a series of DNA ... slightly longer than normal. These repeats are prone to even further ...
Breaking Biology News(10 mins):Can action movies make you fat? 2Nature's tiny engineers 2Nature's tiny engineers 3A nucleotide change could initiate fragile X syndrome 2
... longest in the animal kingdom and they have largely ... scientists led by the University of Exeter have uncovered ... Africa and the results could have huge implications for ... the journal Current Biology, Dr Brendan Godley and an ...
... international team of scientists reported evidence, in a controversial cover ... more than 3.8 billion years ago--400 million years earlier than ... that team and two of the original authors will report ... , Craig E. Manning, lead author of the new study ...
... mouse is providing hope of a new treatment for ... transmission of serotonin in the brain. Serotonin is known ... sexuality. By breeding mice with an absence of TREK-1, ... of this research, which involved an international collaboration with ...
Cached Biology News:How satellite tracking revealed the migratory mysteries of endangered Atlantic loggerhead turtles 2UCLA scientists strengthen case for life more than 3.8 billion years ago 2UCLA scientists strengthen case for life more than 3.8 billion years ago 3UCLA scientists strengthen case for life more than 3.8 billion years ago 4UCLA scientists strengthen case for life more than 3.8 billion years ago 5Ever-happy mice may hold key to new treatment of depression 2
... Pig serum is collected from fasted Guinea Pigs. ... Citrate N-04: Heparin (Na) N-06: ... N-10: EDTA (Na) N-03: Alsevers ... N-09: K3EDTA N-11: Heparin ...
GENOMIPHI V2 DNA AMP KIT 25RXN, 1 EA. Category: Genomics DNA Amplification....
EPH electrode, anode, 1. Category: Electrophoresis Systems & Standards & Reagents, IEF & SDS-PAGE & 2-D Electrophoresis, Multiphor II Kits & Components and Accessories....
... precipitation offers major advantages over other RNA precipitation ... protein or carbohydrates. It is the method of ... synthesis from RNA preparations. Note, LiCl precipitation may ... isolations. If the RNA is to be used ...
Biology Products: