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New Tools for Small RNA Analysis

methods for the detection of small RNAs have not been previously available. RT-PCR, while being very sensitive, cannot be used to amplify small RNAs since in standard RT-PCR, the primers are about the same size as the small RNAs. As a result, siRNAs and miRNAs have been primarily detected by Northern analysis, a technique that is time-consuming and relatively insensitive.


The mirVana System for Small RNA Analysis
To facilitate the analysis of small RNA expression, Ambion has developed the mirVana system, a complete solution for the isolation and detection of small RNAs (Figure 1). The mirVana products include:

The mirVana miRNA Isolation Kit--for isolation of total RNA containing small RNAs, or preparation of fractions enriched in small RNAs (<200 nt).

"miRNA certified" FirstChoice RNAs--human, mouse, and rat total RNAs that have been verified to contain miRNAs and other small RNA molecules

The mirVana miRNA Detection Kit--for the sensitive detection and quantitation of miRNA and other small RNA

The mirVana miRNA Probe Construction Kit--for rapid synthesis of template and RNA probes by in vitro transcription

The mirVana miRNA Probe & Marker Kit--for the 5' end-labeling and purification of RNA or DNA probes, and preparation of small RNA markers

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