Navigation Links
New Tools for Small RNA Analysis


Non-coding small RNAs such as transfer RNAs (tRNA), ribosomal RNAs (rRNA), small nucleolar RNAs (snoRNA), and small nuclear RNAs (snRNA) play critical roles in a variety of cellular processes, from regulation of mRNA translation, pre-mRNA splicing or transcription to chromosome maintenance and gene imprinting. In addition, two novel classes of small non-coding RNAs (1924 nt)--referred to as small interfering RNAs (siRNAs) and microRNAs (miRNAs)--have recently emerged as powerful regulators of gene expression in a variety of organisms (1,2). siRNAs are now widely used as tools for gene silencing experiments in mammalian cells and organisms. Here we describe methods for small RNA isolation, labeling, and analysis.


Analyzing the Expression of Small RNAs
Currently, the analysis of miRNA and siRNA expression suffers from two technological drawbacks. Firstly, most standard RNA isolation protocols were not designed to efficiently recover small RNAs species. For example, glass fiber filters, which are widely used to prepare total RNAs, do not quantitatively recover various RNAs that are smaller than 200 nt (such as 5S rRNA, snRNAs, snoRNAs, tRNAs, miRNAs, and siRNAs). Commercially available total RNA preparations do not always contain representative amounts of small RNA species because the RNA isolation protocols have not been optimized to recover small RNAs. A second technological challenge is that sensitive
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells
2. Tools for Proteomic Science II
3. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
4. Research Tools for Analysis of ZAP-70
5. SNPs Powerful Tools for Association Studies
6. New Tools for Making and Processing Protein Microarrays.
7. Fast Isolation of Total RNA from Small Numbers of Cells
8. Detecting Attomole Amounts of Small RNA
9. Got Small RNA?
10. Rapidly Prepare Probes and Markers for Small RNA Analysis
11. Ultra-Fast Purification of Small Nucleic Acids
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New Tools for Small RNA Analysis

(Date:7/30/2015)... ... ... The 2015 Market Research Report on the Global Propanol Industry is ... with a focus on the Chinese situation. Major companies included in the propanol market ... Royal Dutch Shell, LG Chem and Zhejiang Xinhua Chemical. , The report provides a ...
(Date:7/30/2015)... ... July 30, 2015 , ... Leading regenerative veterinary medicine company, ... canine osteoarthritis stem cell product, currently under development for FDA approval. The product ... marketed in the US by Aratana. This product, termed AT-016, is an allogeneic ...
(Date:7/30/2015)... , Inc. (NASDAQ: SNMX ), a leading company ... commercialize novel flavor ingredients for the food, beverage, and ... second quarter 2015. "Moving into the ... achieve our commercial and financial goals," stated John Poyhonen, ... our last quarterly earnings report, our two partners have ...
(Date:7/30/2015)... 2015 Ascendis Pharma A/S (Nasdaq: ... innovative TransCon technology to address significant unmet medical ... six-month Phase 2 study to evaluate the safety ... 53 treatment-naïve, pre-pubertal children with growth hormone deficiency, ... with the top-line results from our Phase 2 ...
Breaking Biology Technology:Global Propanol Market Technical Data and Manufacturing Analysis 2020 2Global Propanol Market Technical Data and Manufacturing Analysis 2020 3VetStem Biopharma Successfully Completes Large Milestone Efficacy Study for its Flagship Canine Osteoarthritis Stem Cell Product 2VetStem Biopharma Successfully Completes Large Milestone Efficacy Study for its Flagship Canine Osteoarthritis Stem Cell Product 3SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 2SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 3SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 4SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 5SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 6SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 7SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 8SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 9Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 2Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 3Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 4Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 5Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 6Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 7
... Wis. StrandSoft Inc . announced on ... messaging system that allows businesses to use video ... multiple business opportunities without forcing major system alterations, ... offers, restaurant specials, hospital procedures, and health club ...
... A new kind of science is revolutionizing technology, and ... million to harness its potential during the next five ... of materials and processes at scales so small that ... microscope. These materials measure between one and 100 nanometers, ...
... about Midwest technology workplaces in this Nine2Five column, ... Midwest tech employee if you work from home doing outside ... technology is so integrated into our daily work lives nowadays ... the "bleep" noise from the machine is finished. , ,So ...
Cached Biology Technology:Online Kiosks provides a new way to send a message 2UW-Madison gets $13 million to study nanotech 2You might work in a Midwest technology environment if... 2You might work in a Midwest technology environment if... 3
(Date:7/8/2015)... July 8, 2015  Trovagene, Inc. (NASDAQ: ... today announced the launch of a study that ... Cancer Monitoring℠ (PCM) technology for predicting response to ... a combination of the novel immunotherapy agents Yervoy® ... PD-1 inhibitor. The 50-patient study will be led ...
(Date:7/7/2015)... 2015  Based on its recent analysis of ... Credence ID, LLC with the 2015 Asia-Pacific ... Year Award. Credence ID has developed a low-cost, ... of offering enrollment and identification solutions to the ... was formed by experts from the mobile biometrics ...
(Date:7/2/2015)... , June 25, 2015 ... addition of the "Next Generation Biometrics Market by ... 2020" report to their offering. The ... Billion by 2020, at a CAGR of 17.9% between ... the leading application for the market. Safran SA ...
Breaking Biology News(10 mins):Trovagene Initiates Study to Monitor Response to Immunotherapy in Melanoma Patients Using its Precision Cancer Monitoring Platform 2Trovagene Initiates Study to Monitor Response to Immunotherapy in Melanoma Patients Using its Precision Cancer Monitoring Platform 3Trovagene Initiates Study to Monitor Response to Immunotherapy in Melanoma Patients Using its Precision Cancer Monitoring Platform 4Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 2Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 3Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 4Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 5Next Generation Biometrics Market 2015 - Global Forecast to 2020 with Safran, NEC, 3M Cogent, Fujitsu & Suprema Dominating the $24 Billion Market 2Next Generation Biometrics Market 2015 - Global Forecast to 2020 with Safran, NEC, 3M Cogent, Fujitsu & Suprema Dominating the $24 Billion Market 3
... London, containing more than a dozen skeletons of people ... are thought to have died during the 14th century ... the site. The Plague is by definition ... is highly contagious, leading to mass outbreaks across populations. ...
... the Max Planck Institute for Polymer Research (MPI-P) in ... a mineral content of almost 90 percent, yet extremely ... sea sponges and recreated the sponge spicules using the ... sponge. Natural minerals are usually very hard and prickly, ...
... living in the Nebraska Sandhills, color can literally be the ... colonized the region, the dark-coated mice stood out starkly against ... Over the next 8,000 years, however, the mice evolved a ... stripe on their tails and changes in the extent of ...
Cached Biology News:Inspired by deep sea sponges: Creating flexible minerals 2One gene, many mutations 2One gene, many mutations 3
Chicken Anti-Human DNA-directed RNA Polymerase II 7.6 Kd Polypeptide (POLR2L Polyclonal AntibodyOther Sub-Family: not assigned-Other Applications: ELISA, Western Blot...
The BD CBA Human Apoptosis Kit can be used to quantitatively measure,cleaved PARP, Bcl-2, and active Caspase-3 protein levels in a single sample. The,Kit performance has been optimized for analysis o...
... proteases/caspases initiates apoptosis in mammalian cells. ... provides a simple and convenient means for ... in intact cells. The assay utilizes ... linked to rhodamine 110 (D2R), a reported ...
The PROTEAN II slab gel casting stand is a component of the PROTEAN II XL and xi casting apparatus. It is supplied with gaskets....
Biology Products: