( mes and more importantly to achieve t...)New Tools for Making and Processing Protein Microarrays.

mes and, more importantly, to achieve this in a very low volume (75 μL) reaction chamber. The ability to perform multiple, miniaturized assays is particularly advantageous for research that requires expensive reagents, or where only a limited amount of clinical sample or biopsy material is available for analysis.

Since temperature control of any protein assay is essential for reproducibility between a series of experiments, the ProteinArray Workstation has been designed to ensure complete uniformity of temperature to within 0.5C, not only across the protein array during the assay but also between reaction chambers. The samples are stored in a thermally controlled block prior to being introduced into the reaction chamber, so no off-line heating or cooling of the sample is required. There is also precise, stable temperature management of the protein microarray from 4 to 45C, enabling flexibility in assay design.

A key aspect of the ProteinArray Workstation is that it is fully automated. Once reagents and samples are loaded onto the instrument, no further manual intervention is required. Even the introduction of up to 2 low volume (75 μL) samples per protein microarray into the biochip reaction chamber, is performed automatically. The unique ability of the system to automatically introduce two low volume samples enables the system to be used to automate applications such as sandwich assays, where a second sample comprising an antibody that is unique to the assay and may be highly valuable is required. The result is a significant reduction in hands-on time and in the total processing duration, releasing the operator for other activities. Additionally, multiple protein microarrays can be processed in parallel, enabling high throughput, predictable and reproducible performance of assays without scheduling difficulties.

Another unique aspect of the system is its ability to perform on-line mixing of buffers via the use
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