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New Temperature Cycler Features Interchangeable Blocks

these PCR amplifications. Reactions were amplified using the 40-well block format and Hot Top Assembly. The Hot Top Assembly was then changed to the standard paddle and amplifications were performed as with the Hot Top Assembly, except that an oil overlay was added to each reaction. As in the first experiment, multiple reactions were aliquoted from the same master mix and positioned throughout the blocks during each run to test the well-to-well variability. After cycling was complete, 15-l aliquots from each reaction tube were electrophoresed, stained and visualized as before. In this experiment, comparable yield and specificity of products were observed using either the Hot Top Assembly or the standard paddle and an oil overlay on reactions (figure 3). Similar results were obtained using the 96-well block format (data not shown). In all cases, well-to-well variability was low. These results demonstrate that the addition of the Hot Top Assembly to a RoboCycler Infinity temperature cycler fitted with either the 40- or 96-well block format did not adversely affect the formation of PCR products or well-to-well temperature uniformity.

figure 3

Conclusions

The interchangeable block RoboCycler Infinity temperature cycler allows 200-l or 600-l tubes to be run on a single machine. Block sets are currently available in a 40-well and a 96-well format. In addition, a 384-well block set is in development and slated for a 1998 release. The blocks and paddle assemblies are easily and quickly exchanged without the use of tools. PCR results obtained using the RoboCycler Infinity temperature cycler showed similar product yield and specificity regard
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