Navigation Links
New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction,,,Pathways


Monitor activation status of interferon-stimulated signaling pathways

Li Xu Tim Sanchez Mary Buchanan Chao-Feng Zheng
Stratagene

Stratagenes family of PathDetect cis-reporting systems now includes the GAS and ISRE reporting systems, to better study the activation of signal transduction pathways stimulated by interferon - a, - b, or - g. The pISRE-Luc cis-reporter plasmid contains direct repeats of the interferon-stimulated response element (ISRE) found in the promoter of the 54-kDa interferon-stimulated gene (ISG54); the pGAS-Luc cis-reporter plasmid contains direct repeats of the gamma-activating sequence from the promoter of the guanylate-binding protein. In these plasmids, a basic promoter element (TATA box), and the direct repeats of ISRE or GAS, control expression of the luciferase gene. The pISRE-Luc and pGAS-Luc reporter plasmids monitor the activation status of signaling molecules and transcription factors that mediate the function of interferons.

Interferons (IFNs) are soluble proteins that act as local hormones, inhibit viral replication, and activate certain host defense responses. These interesting proteins modulate the expression of many genes and elicit a wide range of biological functions, including tissue repair, activation of various blood cells involved in antibody production, inflammation, and host defense against viruses.2,3 IFNs have also been used as therapeutic agents against many viral diseases, including acquired immune-deficiency syndrome (AIDS).1,2,3

IFN-a and IFN-b are interferons synthesized by many cell types following viral infection and have two major functions: They inhibit viral replication by activating cellular genes whose products are
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
2. Stable HeLa Luciferase Reporter Cell Lines Expressing GAL4 Fusion Transactivators
3. Versatile Reporter Vectors for Monitoring Viral Transduction
4. Genetic Reporter Systems
5. Genetic Reporter Systems
6. Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment
7. A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20)
8. Luciferase Reporter Gene Cells and Recombinant Luciferase Dilutions Studied with the CLIPR Luciferase Assay Kit and the LMax Microplate Luminometer (MaxLine Application Note #38)
9. Promegas Multiplexed Luciferase Reporter and Cell Viability Assays performed on the PHERAstar
10. New Fusion Trans-Activator Plasmids for Studying Signal Transduction Pathways
11. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
Post Your Comments:
(Date:5/28/2015)... 2015 Research and Markets( http://www.researchandmarkets.com/research/3nl6s2/neuroprotection ) ... report "Neuroprotection - Drugs, Markets and Companies" ... describes the role of neuroprotection in acute disorders ... system as well as in chronic diseases such ... mechanisms of damage to neural tissues are similar ...
(Date:5/28/2015)... 2015 Celsion Corporation (the "Company") (NASDAQ: ... from two institutional healthcare investors to purchase an aggregate ... in an at-the-market registered direct offering and a concurrent ... The Company entered into a definitive purchase agreement ... to sell an aggregate of 3,000,000 shares of its ...
(Date:5/28/2015)... 28, 2015 DryLet, LLC a ... applications such as animal waste reduction, bioremediation, wastewater ... in wastewater treatment plants and restaurant kitchen settings, ... Pork Expo, June 3-5 in Des Moines, Iowa. ... a Wall Street Journal article because of its ...
(Date:5/28/2015)... BRUNSWICK, N.J. , May 28, 2015 /PRNewswire/ ... PDS0101, a cancer immunotherapy treatment targeting HPV-16, has ... Currently, pre-cervical cancer is treated ... offer a simple and effective non-surgical alternative. Results ... defense mechanisms (T-cells) to recognize, target, and kill ...
Breaking Biology Technology:Global Neuroprotection Market Report 2015-2024 - Drugs, Markets and Companies 2Global Neuroprotection Market Report 2015-2024 - Drugs, Markets and Companies 3Celsion Corporation Announces $8 Million At-The-Market Registered Direct Offering 2Celsion Corporation Announces $8 Million At-The-Market Registered Direct Offering 3DryLet to Showcase Patented ManureMagicâ„¢ at the 2015 World Pork Expo, June 3-5 in Des Moines, Iowa 2PDS Biotechnology reports positive Phase I clinical trial results in proprietary HPV-Cancer Immunotherapy 2
... INTRODUCTION , ... agonist, a transient increase in intracellular calcium concentration. This variation ... modulator of many physiological mechanisms (reviewed by Rink (1990), Tsunoda ... calcium concentration in cells expressing a GPCR can thus be ...
... Heidi Grecsek , ... 710 Bridgeport Avenue , Shelton, ... The Clean Air Act amendments of 1990 require ... Oxygenated fuel is desirable because it burns more completely, thereby reducing tailpipe emissions. The ...
... Author: , Thomas Wampler , ... 465 Limestone Road , Oxford, PA ... The use of analytical pyrolysis 1 with gas chromatography/ mass ... of how these large molecules behave at elevated temperatures. The utility of the technique is ...
Cached Biology Technology:Cell lines expressing recombinant aequorin and a G-protein coupled receptor for functional screening 2Cell lines expressing recombinant aequorin and a G-protein coupled receptor for functional screening 3Cell lines expressing recombinant aequorin and a G-protein coupled receptor for functional screening 4Cell lines expressing recombinant aequorin and a G-protein coupled receptor for functional screening 5Cell lines expressing recombinant aequorin and a G-protein coupled receptor for functional screening 6Cell lines expressing recombinant aequorin and a G-protein coupled receptor for functional screening 7Cell lines expressing recombinant aequorin and a G-protein coupled receptor for functional screening 8Analysis of Fuel Oxygenates by U.S. EPA Method 8260B Using Headspace Trap with GC/MS 2Analysis of Fuel Oxygenates by U.S. EPA Method 8260B Using Headspace Trap with GC/MS 3Analysis of Fuel Oxygenates by U.S. EPA Method 8260B Using Headspace Trap with GC/MS 4Polymer Degradation Mechanisms Encountered in Pyrolysis-GC/MS 2Polymer Degradation Mechanisms Encountered in Pyrolysis-GC/MS 3Polymer Degradation Mechanisms Encountered in Pyrolysis-GC/MS 4
(Date:5/20/2015)... , May 20, 2015 NXT-ID, Inc. ... that its wholly owned subsidiary, 3D-ID LLC, a company ... Team Battelle for the biometrics technology portion of the ... Contract from the Department of the Army. ... products developed for government, law enforcement and security agencies. ...
(Date:5/19/2015)... NEW YORK , May 19, 2015  Technology ... resources both internally and in the cloud. Passwords and ... the use of OTP and standards-based specifications such as ... that the outmoded use of passwords presents for BYOD, ... needs a unified biometric identity protocol. ...
(Date:5/18/2015)... , May 18, 2015 Fingerprint Cards ... FPC1020, FPC1025, FPC1145, FPC1155 and FPC1035 from one if its ... until and including Q3 2015 and the sensors will be ... Communicated order values for 2015 hereby amount to 740 MSEK ... 2015 of 140 MSEK and a number of smaller orders ...
Breaking Biology News(10 mins):NXT-ID, Inc Announces Participation as Member of Team Battelle for TIES Contract Award 2NXT-ID, Inc Announces Participation as Member of Team Battelle for TIES Contract Award 3HYPR Corp. Biometric Security Integration Kit Shields Applications and Devices Across the Internet of Things 2HYPR Corp. Biometric Security Integration Kit Shields Applications and Devices Across the Internet of Things 3Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 230 Million and Raises Revenue Guidance for 2015 2
... BIRMINGHAM, Ala. University of Alabama at Birmingham (UAB) ... new book that claims it,s possible to teach evolution ... the theory. In his book, The Missing ... Evolution, Meadows, a Christian and science educator, writes: "For ...
... The University of North Carolina at Chapel Hill is ... part of an unprecedented large-scale, collaborative effort by the National ... (NHGRI) to systematically characterize the genomic changes that occur in ... a five-year award, expected to bring $13 to $20 million ...
... of the University of Massachusetts Medical School today announced ... the safety and activity of a human monoclonal antibody ... clinical trial is being conducted at the King Edward ... the Serum Institute of India, which is working in ...
Cached Biology News:UAB professor's book promises solution for teaching evolution without conflict 2UNC awarded Cancer Genome Atlas grant 2First human gets new antibody aimed at rabies virus 2First human gets new antibody aimed at rabies virus 3
... EnVision™ G|2 Doublestain System is ... generation visualization kit. The kit is ... the simultaneous detection of two different ... is compatible with suitably diluted rabbit ...
Liquid. PBS containing 10 mM NaN 3 . AVOID FREEZE/THAW CYCLES. Recognizes α1, 2, 3, 5, 6 and 7 subunits of 20S proteasome. ...
AVOID FREEZE/THAW CYCLES. Recognizes the UCP-2 protein....
Mouse monoclonal antibody to Lats2....
Biology Products: