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New Protocols for Isolating High- Molecular-Weight Genomic DNA

h uses a modified phenol-chloroform extraction and an ethanol precipitation protocol optimized for isolating high-molecular-weight genomic DNA from transgenic animals containing lambda shuttle vectors. The Big Blue DNA isolation kit was the first kit specifically designed to prepare genomic DNA of size and quality that, when subjected to in vitro packaging extracts, yielded large numbers of rescued shuttle vector.

Table 1
Average Lambda Phage Recovery with RecoverEase DNA Isolation Kit and Organic Extraction Methods

Tissue

Tissue source

Method

Average yieldb

Average pfu/reactionc

Average total pfu recoverabled

Average pfu/mg DNAe

Blood

Big Blue rats

Dialysis
(RecoverEase kit)

45 mg

226,000

3,892,000

86,000

Organic Extraction
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Source:


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