Navigation Links
New Protocols for Isolating High- Molecular-Weight Genomic DNA


Rapidly recover intact DNA from blood, bone marrow, and cultured cells

Brenda J. Rogers Mark J. Dycaico
Stratagene

Stratagene now offers updated protocols to isolate and purify high-molecular-weight genomic DNA using the RecoverEase DNA isolation kit. New procedures include DNA isolation from blood, bone marrow, and tissue culture cells. In addition, protocols for liver, lung, kidney, spleen, testes, and brain are also included. Each procedure eliminates the need for toxic phenol or chloroform and requires very little hands-on time to perform. Use the kit to minimize physical manipulation of the DNA the point in purification methodology where DNA is most susceptible to shearing forces. Fully hydrated high-molecular-weight DNA is ready to use in molecular biological applications in less than 24 hours.

The RecoverEase DNA isolation kit is designed to isolate very high-molecular-weight genomic DNA by reducing the shearing forces that take place in traditional methods using phenol-chloroform extraction and ethanol precipitation. The basic kit procedure requires tissue homogenization, filtration through a filter unit supplied with the kit to remove cellular debris (<3 minutes), isolation of the cell nuclei by centrifugation (<20 minutes), and protease digestion of the nuclei (45 minutes).1,2 The resulting suspension is transferred to a free-floating RecoverEase kit cup where the digested proteins and low molecular weight contaminants are removed by dialysis (16 to 48 hours), leaving behind fully hydrated DNA, which is ready for immediate use. From previous experiments using field inversion analysis, DNA isolated in this way, produced products that were 500-kb long.2 Subsequent Southern blot analysis demonstrated that the DNA cut with a variety of restriction enzymes (data not shown). These benefits, along w
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
2. Eppendorf Multiporator Transfection Protocols for Eukaryotic Cells
3. Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler
4. High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
5. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
6. Highest Possible Transformation Efficiencies for High-Throughput Applications
7. High-Throughput Cultivation of Bacterial Clones
8. Safe Production of High-Titer Retrovirus
9. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
10. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
11. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New Protocols for Isolating High Molecular Weight Genomic DNA

(Date:5/19/2013)... MA (PRWEB) May 19, 2013 The ... social media training online, has proudly announced that the ... small business has surpassed 175,000 views on YouTube. The ... social media and SEO tips and training for small ... my students find the YouTube tips helpful for their ...
(Date:5/17/2013)... German . ... eye, you can see which branch a bird is sitting ... through the telephoto lens for high-magnification images requires considerable skill. ... to study proteins, the active biomolecules of cells. , Olga ... Systems Biology, and her colleagues have now come up with ...
(Date:5/17/2013)... PALO ALTO, Calif. , May 17, 2013 ... that it has achieved 50% enrollment of the ... trial to evaluate the safety and preliminary efficacy ... Progenitor Cells) therapy for Knee Osteoarthritis (KOA). To ... Effect (SAE) reported. The Phase I ...
(Date:5/17/2013)... The paradigm of ‘one drug, one ... can help predict the adverse and therapeutic effects of ... of Computational Biology at the Genomics Laboratory, Covance, will ... high-quality genomics when used as part of the QC ... data sets to identify key clinical targets even in ...
Breaking Biology Technology:YouTube Channel on SEO Tips Surpasses 175,000 Views, Announces JM Internet Group 2Proteome atlas for the tuberculosis pathogen 2Proteome atlas for the tuberculosis pathogen 3Cellular Biomedicine Group Achieves 50% Enrollment Milestone in Phase I Clinical Trial for Knee Osteoarthritis 2
... RESEARCH TRIANGLE PARK, N.C., Jan. 9 Quintiles,Transnational ... its,Global Central Laboratories and Clinical Development Services (CDS) ... China., The new consolidated Quintiles China facility, ... located at Sun Dong Ann Plaza in Beijing, ...
... Burrill Merchant Banking acted as,exclusive financial advisor ... (teduglutide) with Nycomed.,Under the terms of the agreement, ... to Nycomed for the treatment of gastrointestinal disorders., ... for the treatment of,short-bowel syndrome (SBS). SBS can ...
... MATEO, Calif., Jan. 9 NeurogesX, Inc.,(Nasdaq: NGSX ... therapies, today announced that it completed a $25.0,million private ... of,its late stage portfolio of novel pain management therapies., ... on Sunday, December,23, 2007, it entered into a securities ...
Cached Biology Technology:Quintiles Central Lab, Clinical Development in China to Expand, Relocate to New Facility 2Burrill Merchant Banking Advises NPS on Phase III Licensing Agreement 2NeurogesX Closes $25 Million Private Placement 2NeurogesX Closes $25 Million Private Placement 3NeurogesX Closes $25 Million Private Placement 4
(Date:5/17/2013)... Sustainable Global Logistics Operations) is aiming to cut CO2 ... intermodal loading systems and the boosting of intelligent systems ... with experience in the logistics sector and in supply ... a duration of three and a half years. , ... components and ontologies shared by the users, objects and ...
(Date:5/16/2013)... 2013) Illustrating a commitment to the support ... Association (AGA) Research Foundation has announced the inaugural ... Award recipients. Supported by the National Institute of ... new award helps underrepresented minority students to further ... research. , "By establishing this new award, AGA ...
(Date:5/16/2013)... scientists have used ancient shorelines to predict the stability of ... a high shoreline from three million years ago, for example ... were thought to be evidence of a high sea level ... has led many scientists to think that if the world,s ... do just the same in our modern, progressively warming world. ...
Breaking Biology News(10 mins):New logistics services that will cut energy consumption and CO2 emissions 2Underrepresented minority students receive fellowships in digestive disease and nutrition research 2World's biggest ice sheets likely more stable than previously believed 2
... new study from the Bradley Hasbro Children’s Research Center ... the greatest risk for HIV. Amongst a group of ... for the disease (those who engaged in the most ... health problems. , Prior studies have found that risky ...
... effort was launched last week to develop and use ... avoid potential rice shortages, or even future famine. , ... approach since the early 1990s. But new knowledge generated ... researchers for the first time to discuss how they ...
... beans, red peppers and broccoli as good sources of ... Joy Dubost, who recently earned her doctorate in food ... antioxidants, polyphenols and ergothioneine, present in mushrooms, using the ... dissertation research. She found that portabella mushrooms had an ...
Cached Biology News:New HIV study identifies high-risk subgroups of adolescents 2New HIV study identifies high-risk subgroups of adolescents 3A more powerful and efficient engine for rice: the C3-C4 challenge 2Mushrooms as good an antioxidant source as more colorful veggies 2
...
Jak3 Antibody Ship: Hot Store: -20 C...
WASF2 Immunogen: WASF2 (NP_008921, 73 a.a. ~ 173 a.a) partial recombinant protein with GST tag. Storage: -20 C, Avoid Freeze/Thaw Cycles *Available for distribution in the US only...
Applications: Western blotting Stability: Room temperature, indefinitely ...
Biology Products: