Navigation Links
New PCR Primers for Human Diseases

Figure 1

Figure 2

Each primer set was used in PCR amplifications of DNA templates from infected serum samples, with genomic DNA from infected cells or with cDNA made from RNA isolated from infected cells. The control reactions were performed with DNA from both uninfected sera and human cells. The specificity of each primer set is shown in figure 1. Each amplification yielded a single product of the appropriate size in reactions with DNA from infected serum samples (figure 1 and figure 2), whereas amplification products were not detected in any of the negative controls, uninfected serum samples (figure 1).

Assay for the Factor V Mutation

The factor V (Leiden) mutation is associated with thrombophilia. In order to test for a point mutation of G1691A, the gene coding for human coagulation factor V, the factor V (Leiden) mutation assay is used. This assay requires an initial PCR amplification with subsequent digestion with the Mnl I restriction enzyme that yields a restriction fragment length polymorphism for the wild-type, heterozygous and homozygous genotypes (figure 2). Stratagenes primer set for performing the factor V mutation assay includes the Mnl I enzyme and the corresponding
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
2. Gene-Specific Primers for NMDA Receptor Subunits
3. RT-PCR Primers for the Study of Apoptosis
4. Amplification of Epstein-Barr Virus Exon C with Difficult Primers Using Eppendorf Mastercycler gradient
5. Human Primary Preadipocytes and Differentiated Adipocytes
6. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
7. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
8. Human Universal cDNA Library Array I
9. Map and Link Human Genetic Disorders with SSLP Analysis
10. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
11. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New PCR Primers for Human Diseases

(Date:12/17/2014)... (PRWEB) December 16, 2014 “Never ... at Philipps- Universität Marburg’s Department of Physics, advises ... in the newly released SPIE Women in ... be too shy to ask questions.” , In ... engineering, and mathematics) occupations ranging from university professor ...
(Date:12/17/2014)... BASKING RIDGE, N.J. (PRWEB) December 17, 2014 ... Ipsen (Euronext: IPN; ADR: IPSEY), ... 120 mg (referred to as Somatuline®) was approved ... for the treatment of gastroenteropancreatic neuroendocrine tumors (GEP-NETs) ... differentiated, locally advanced or metastatic disease to improve ...
(Date:12/17/2014)... December 17, 2014 This report ... Quartz Tubing industry. For an overview analysis, the ... application, industry chain structure, industry overview, policy analysis, ... tubing industry has witnessed rapid development with ... of acquisitions. This report mentions quartz tubing upstream ...
(Date:12/17/2014)... (PRWEB) December 17, 2014 Gene ... that it has entered into a technology access ... (ADM) to apply DNA2.0’s proprietary protein engineering technology, ... process. , “We are extremely excited that the ... platform. This proprietary bioengineering technology has now ...
Breaking Biology Technology:Network, Multitask, Persevere: SPIE Women in Optics Planner Subjects Share from Experience 2Network, Multitask, Persevere: SPIE Women in Optics Planner Subjects Share from Experience 3Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 2Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 3Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 4Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 5Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 6Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 7Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 82020 Quartz Tubing Market: Global Development & Forecasts Analysis Now at DeepResearchReports.com 2ADM and DNA2.0 Enter Into Protein Engineering Technology Access and Service Agreement 2
(Date:11/21/2014)... According to a new market research report ... Control, Intrusion Detection, Parking Management, Under Vehicle Inspection), End ... - Global Forecasts to 2020", published by MarketsandMarkets, The ... $25 Billion in 2014 and is expected to reach ... 8.69%. Browse 116 market data Tables and ...
(Date:11/18/2014)... Nov. 18, 2014  The Secure Identity & ... today jointly announce the formation of The Airport ... its Identity and Biometric Entry and Exit ... received official support from BORDERPOL, the international non-profit ... and provide expertise regarding border security, traveler and ...
(Date:11/12/2014)... Nov. 12, 2014 Crossmatch™, a leading provider ... U.are.U ® fingerprint readers have been deployed throughout ... Central Mexico . The bakery chain implemented ... payroll issues caused by employees clocking in for each ... fingerprint readers, Montparnasse relied on paper timecards and a ...
Breaking Biology News(10 mins):Industrial Security Systems Market Worth $38 Billion by 2020 2Industrial Security Systems Market Worth $38 Billion by 2020 3Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 2Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 3Montparnasse Pasteleria Achieves Time and Attendance Transparency with Crossmatch U.are.U Fingerprint Readers 2
... 12th International Coral Reef Symposium (ICRS 2012), the world,s ... years, will begin Monday, July 9, in Cairns, Queensland, ... policy makers, managers, and conservationists to present the latest ... the knowledge and findings presented during the symposium, daily ...
... superinfection may be as common as initial HIV infection ... a new study led by researchers at the Johns ... Institute of Allergy and Infectious Diseases (NIAID). In the ... heterosexual population, researchers examined the rate of superinfection among ...
... report that the parasitic ,Varroa, mite has caused the ... colonies. This association is now thought to contribute to ... honey bee colonies. The current monetary value of honey ... is estimated at about $15-$20 billion annually, The research ...
Cached Biology News:Coral reef experts to present latest coral reef science during July symposium 2Coral reef experts to present latest coral reef science during July symposium 3New data suggests HIV superinfection rate comparable to initial HIV infection 2New data suggests HIV superinfection rate comparable to initial HIV infection 3
... depletion cocktail is tailored to ... suspensions of rat spleen cells. ... StemSep (or compatible 0.5 Tesla) ... T cell content of the ...
CleanCut agarose is used to prepare genomic DNA plugs for use in pulsed field gel electrophoresis (PFGE) applications. Supplied as a 12 ml solution of 2% agarose....
... an intelligent full microplate washer with a 96 ... instrument gives you the best of two worlds ... , Performance ,Separate wash and aspiration ... A dispense precision of better than 2% CV ...
... culture scale-up, these culture chambers produce cell ... 9 range and are useful alternatives ... • High mechanical • strength and structural ... shipping • Certified nonpyrogenic and sterilized by ...
Biology Products: