Navigation Links
New Microarray Labeling Kit for Preparing cDNA Probes

bridize to human genes.

The three A. thaliana mRNA are added to the probe-labeling reaction at three different concentrations, along with test or control mRNA and labeled nucleotides provided by the researcher, to generate fluorescently cDNA probes. The probes are then hybridized to the Control Microarray to determine the effectiveness of the probe labeling and the specificity and sensitivity of the hybridization reactions.

Target DNA on Control Microarray

The Control Microarray is designed to assess mRNA quality to enable researchers to identify problems associated with poor quality mRNA before hybridizing the cDNA probe to an expensive microarray. The Control Microarray contains positive-control target DNA, a negative control, and additional human target DNA (Figure 1). The positive-control target DNA on the Control Microarray are A. thaliana RUBISCO activase (rca), chlorophyll a/b-binding protein, ribulose-1,5-biphosphate carboxylase/oxygenase large subunit (rbcL) genes, and the human -actin gene. Two samples containing only 3X SSC are provided as negative controls.

The Control Microarray also contains six additional human target DNA. These target DNA are expressed in different tissues at varying abundances and provide additional information regarding the quality of the labeled cDNA probe and efficiency of the hybridization.

Specificity and Sensitivity of the Assay

The A. thaliana control mRNA and target DNA on the Control Microarray can be used to determine the sensitivity of the assay. The A. thaliana-labeled probe is generated by adding the A. thaliana mRNA provided in the probe-labeling kit to the probe-labeling reaction. The amounts of A. thaliana photosystem I chlorophyll a/b-binding protein, rca and rbcL mRNA
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Stratagenes Human cDNA Microarrays: Perform Gene Expression Profiling and Discover New Genes
2. An Optimal DNA Microarray Substrate for the Identification of Fetal and Somatic Liver Stem Cells of the Rat
3. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
4. Microarray Target Purification Kit and the Microarray Target Synthesis Kit (T7)
5. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
6. Increased Sensitivity in Microarray Analysis
7. Analysis of Microarray Data
8. Cancer-Related miRNAs Uncovered by the mirVana miRNA Microarray Platform
9. Is RNA Amplification Necessary for Microarrays?
10. Perform Microarray Analysis with Limited Bacterial RNA
11. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New Microarray Labeling Kit for Preparing cDNA Probes

(Date:11/21/2014)... 21, 2014 Why did Stephen Hawking ... Einstein so instantly recognizable? Why have they become icons ... and author Hilton Ratcliffe seeks out the answers to ... all to do with science. In " Stephen Hawking ... November 21, 2014), Ratcliffe puts it plainly: , ...
(Date:11/21/2014)... , Nov. 20, 2014  Cytori Therapeutics (NASDAQ: ... Hedrick , M.D. will present live at VirtualInvestorConferences.com on December ... TIME:    11:15 am PT / 2:15 pm ET ... into your browser,s address bar: http://bit.ly/1B2KGaL ... receive event updates. This will be a ...
(Date:11/18/2014)... Urdorf, Switzerland (PRWEB) November 17, 2014 ... range of processes across the pharmaceutical, chemical, and food ... is it so important, and how do pH sensors ... measurement to assist those in process industries with these ... description of pH measurement in the process industries. The ...
(Date:11/18/2014)... November 18, 2014 2014 ... Industry is professional and in-depth report on ... basic information, including its definition, characterization, application, ... outline. This exploration covers the worldwide business ... industry examination covering macroeconomic environment & financial ...
Breaking Biology Technology:“Stephen Hawking Smoked My Socks” Sparks Controversy in Science World 2“Stephen Hawking Smoked My Socks” Sparks Controversy in Science World 3“Stephen Hawking Smoked My Socks” Sparks Controversy in Science World 4Cytori Therapeutics to Provide Corporate Update and Investor Presentation on December 4 through a Live, Interactive Webcast 2Cytori Therapeutics to Provide Corporate Update and Investor Presentation on December 4 through a Live, Interactive Webcast 3Cytori Therapeutics to Provide Corporate Update and Investor Presentation on December 4 through a Live, Interactive Webcast 4pH Guide from METTLER TOLEDO Explains Measurement, Theory and Practice of pH Applications 2PTMEG Market: 2019 Global Polytetrahydrofuran Industry Development Trends & Analysis Now at DeepResearchReports.com 2PTMEG Market: 2019 Global Polytetrahydrofuran Industry Development Trends & Analysis Now at DeepResearchReports.com 3
... South Korean scientists have succeeded in engineering the bacterium ... research, published in the journal Biotechnology and Bioengineering ... this important chemical which is traditionally created using fossil ... is an important platform chemical with a wide range ...
... - Net Profit Up 11%, - Strong Research and Investment Effort, - Positive net ... 25 August 2009 under the chairmanship,of Albert Saporta approved the 2009 half year financial statements: , , In million Euros ... ... %CA, Sales ...
... , , SAN MARINO, ... company that develops drug compounds for HIV/AIDS, autoimmune diseases, and cancer, ... releases distributed by collegestock.com on August 25 and August 26, and ... Reader Grapevine: There,s Something About Viral Genetics, Inc. (VRAL)," on August ...
Cached Biology Technology:Pioneering research succeeds in producing industrially vital chemical through engineered bacteria 2STALLERGENES: 1st Half-Year 2009: Performance Ahead of Expectations 2STALLERGENES: 1st Half-Year 2009: Performance Ahead of Expectations 3STALLERGENES: 1st Half-Year 2009: Performance Ahead of Expectations 4Viral Genetics Responds to Rumors of Imminent FDA Filing and Third Party Press Releases 2Viral Genetics Responds to Rumors of Imminent FDA Filing and Third Party Press Releases 3
(Date:11/6/2014)... an asset for a predator. Except when that predator runs ... beetle, relative to its size, is the fastest creature on ... lengths per second (at about five miles per hour). The ... the sprinting gold from the tiger beetle, a person would ... tiger beetle has a problem. At peak speeds, everything becomes ...
(Date:11/5/2014)... in the UTSA College of Sciences, is one ... receive a two-year $300,00 National Science Foundation (NSF) ... funding supports President Obama,s BRAIN Initiative, a federal ... that will demystify complex brain processes. , ... broken into the interactions of multiple components, each ...
(Date:11/5/2014)... diversity in their ability to identify scents and odors. ... their perceptual evaluation of odors, with women outperforming men ... Sex differences in olfactory detection may play a role ... one,s perception of smell, which is naturally linked to ... been suggested to be cognitive or emotional, rather than ...
Breaking Biology News(10 mins):The tiger beetle: Too fast to see 2UTSA biology professor awarded $300,000 NSF grant for brain research 2UTSA biology professor awarded $300,000 NSF grant for brain research 3The female nose always knows: Do women have more olfactory neurons? 2
... will not only teach us more about the way neurons ... diagnostics and therapies for neurological diseases such as Parkinson,s," says ... take a major toll on society. More than 8% of ... cent suffers from a mental disturbance and the number of ...
... is now leading to breakthroughs in bio nanotechnology research. ... reality. Four years ago, the biologist Karen ... when she started with her team working on it, ... at the Nano-Science Center, her team has been able ...
... the first time, the successful reprogramming of diseased human ... also found differentiation into mature hepatocytes was more efficient ... diseased hepatocyte-derived human iPSC lines provides a good basis ... technology could give a potentially unlimited reservoir of cells ...
Cached Biology News:Skywalker ensures optimal communication between neurons 2From science fiction to research breakthrough 2
... Description: Proteinase K is a ... wide range of applications purified from the fungus ... the carboxyl group of N-substituted hydrophobic aliphatic and ... as a serine protease. Proteinase K is useful ...
... high throughput, water bath thermal cycler capable of ... This equates to 9,216 reactions per run when ... transfers a basket of plates between three water ... considerably faster than those of peltier block cyclers. ...
... Dideoxycytidine Dideoxycytidine is a cytidine analog that ... retroviral activity. Immunogen: Chemical / ... The specificity of the ddC antiserum was ... of ddC to moles of ddC analog at ...
... The Jouan MSC series of Class ... working conditions and certification to the ... regulation ensures that the cabinets cannot ... conditions. Natural lighting, low noise and ...
Biology Products: