Navigation Links
New Microarray Labeling Kit for Preparing cDNA Probes


A more reliable and reproducible way to create fluorescently or radioactively labeled cDNA

David T. Wong Heidi Davis Darryl Garrison Terry Payette Matt Lundy
Kim Kuplent Mary A. Buchanan Joseph A. Sorge Rebecca L. Mullinax
Stratagene

Travis Ward Andrew Firmin
Phenogenex

Nucleic acid microrrays, which contain hundreds or thousands of genes in a single format, are increasingly being used to identify differentially expressed genes. In this process, test and control mRNA are converted into cDNA probes in a reverse transcription reaction that incorporates labeled nucleotides. The test and control cDNA probes are then hybridized to arrays, the unhybridized probe is removed, and the hybridization is detected. Differences in the levels of hybridization signals of specific genes on the array correlate with differences in the abundance of those genes in the test and control mRNA used to prepare the probes.

We have developed the Microarray Labeling KitPP for use with gene microarrays1 to increase the reliability and reproducibility of identifying differentially expressed genes. The kit includes several control mRNA and two Control Microarrays designed to assess the mRNA quality and the sensitivity and specificity of the assay.

Arabidopsis Control mRNA

The Microarray Labeling Kit includes three artificially generated mRNA from Arabidopsis thaliana genes. They are A. thaliana RUBISCO activase (rca), chlorophyll a/b-binding protein, and ribulose-1,5-biphosphate carboxylase/oxygenase large subunit (rbcL) genes. These genes were selected because they are involved in plant-specific processes and do not have known human homologues. In addition, they do not hybridize to membrane-arrayed human cDNA from 29 different tissues. Therefore, cDNA probes generated from these genes are unlikely to hy
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Stratagenes Human cDNA Microarrays: Perform Gene Expression Profiling and Discover New Genes
2. An Optimal DNA Microarray Substrate for the Identification of Fetal and Somatic Liver Stem Cells of the Rat
3. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
4. Microarray Target Purification Kit and the Microarray Target Synthesis Kit (T7)
5. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
6. Increased Sensitivity in Microarray Analysis
7. Analysis of Microarray Data
8. Cancer-Related miRNAs Uncovered by the mirVana miRNA Microarray Platform
9. Is RNA Amplification Necessary for Microarrays?
10. Perform Microarray Analysis with Limited Bacterial RNA
11. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New Microarray Labeling Kit for Preparing cDNA Probes

(Date:6/30/2015)... ... June 30, 2015 , ... Matrix Marketing Group, ... it has hired Gem Swartz to head up its account management department as vice ... Both Gem and Amanda bring senior account management and delivery expertise to the growing ...
(Date:6/30/2015)... ... 30, 2015 , ... Southern Illinois University Edwardsville Chancellor Julie ... president of instruction at Chippewa Valley Technical College (CVTC) and will assume her ... lead SIUE during the past three years,” Furst-Bowe said. “I want to thank ...
(Date:6/29/2015)... KS (PRWEB) , ... June 29, 2015 , ... ... PATENT 7736892 B2. Under the terms of the agreement, MediVet will acquire the ... Stem Cells (UCMSCs). MB-007 is currently in pre-clinical development as a potential next-generation ...
(Date:6/29/2015)... 29, 2015  Neuralstem, Inc. (NYSE MKT: CUR), a ... small molecule and cell therapy treatments for central nervous ... for listing on the NASDAQ Capital Market under the ... trade on the NYSE MKT until the market close ... Market is expected to commence on July 13, 2015. ...
Breaking Biology Technology:Matrix Marketing Group Expands Client Services Team 2SIUE’s Furst-Bowe Accepts VP of Instruction at CVTC 2SIUE’s Furst-Bowe Accepts VP of Instruction at CVTC 3MediVet Biologics Acquires Exclusive Patent License from Kansas State University 2MediVet Biologics Acquires Exclusive Patent License from Kansas State University 3Neuralstem, Inc. Announces Approval for Listing on the NASDAQ Capital Market 2Neuralstem, Inc. Announces Approval for Listing on the NASDAQ Capital Market 3
... by the British Heart Foundation (BHF), has tested a new ... patient,s risk of having a heart attack (1). ... Research Excellence, in collaboration with the University of Cambridge are ... CT scanning to image the disease processes directly in the ...
... age of high-speed computing, the photon is king. However, producing ... time-consuming process, until now. Thanks to the work by ... The Edward S. Rogers Sr. Department of Electrical & Computer ... the production of special class of photons faster and easier. ...
... ALBANY, N.Y., April 30, 2012   AMRI ... an approach that further focuses its research and ... the life sciences and other industries. AMRI SMARTSOURCING™ ... customers to reach more successful outcomes. SMARTSOURCING™ is ...
Cached Biology Technology: Scientists develop new technique that could improve heart attack prediction 2Research breakthrough takes supercomputing out of the lab 2AMRI Launches SMARTSOURCING™ For Contract Manufacturing and Research Services 2
(Date:6/23/2015)... , June 23, 2015  Crossmatch™, ... and authentication solutions, today announced enhanced functionality of ... authentication solution.  The enhancements build on the ... the DigitalPersona Altus platform and provide expanded ... In today,s environment of increasing ...
(Date:6/17/2015)... Germany , June 17, 2015 ... Standard: QIA) today launched new Investigator ® STR assay ... the United States . The new genetic ... key genomic markers (short tandem repeats or STRs) for DNA ... evaluate the quality of DNA in each sample, a novel ...
(Date:6/16/2015)... 16, 2015  With the increasing number and severity ... top concern. The recent compromise of Federal employee ... strong authentication within government agencies. HYPR Corp. ... password (OTP) authenticator, has been submitted for testing and ... 3 validation for tamper proofing. The ...
Breaking Biology News(10 mins):Crossmatch Releases Enhanced DigitalPersona Altus Advanced Authentication Functionality 2QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 2QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 3QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 4QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 5Government Ready Biometric Security Approaching as HYPR Corp. Files FIPS 140-2 Level 3 Validation for Its Proprietary Biometric Token 2Government Ready Biometric Security Approaching as HYPR Corp. Files FIPS 140-2 Level 3 Validation for Its Proprietary Biometric Token 3
... 24, 2010 The National Science Foundation this week ... American Society for Biochemistry and Molecular Biology to establish ... nation. The initiative aims to improve undergraduate-level teaching ... and skills for the discipline and by developing appropriate ...
... MADISON, WI, June, 2010 The approach the ... gas emissions from agricultural anaerobic lagoons that treat manure ... to 65%, according to scientists from the University of ... feeding operations prior to application to crops as a ...
... the earth,s atmospheric composition are likely to alter plant ... reach levels double those of the preindustrial era by ... food for a rapidly growing population. University ... carbon dioxide, elevated ozone and higher atmospheric temperatures on ...
Cached Biology News:NSF grant will develop new online hub for biochemistry, molecular biology educators 2Scientists question EPA estimates of greenhouse gas emissions 2Climate change complicates plant diseases of the future 2Climate change complicates plant diseases of the future 3
BBS/Glycerol/Casein Block and Diluent - 10X Concentrate, 1 L...
Ms anti-Cyclophilin D...
From micro to giga sizes, from single prep to high-throughput, Invitrogen's range of plasmid purification kits offers superior performance to match the demands of your downstream application....
... Upright Freezer Inventory Storage Racks ... configurations available to fit all makes ... refrigerators. Made of high quality ... 3" high fiberboard, stainless steel or ...
Biology Products: