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New Mammalian Two-Hybrid System Detects Protein-Protein Interactions

than interactions observed in a foreign environment. The confirmation eliminates the possibility of a false positive that is an artifact from working in yeast cells or in vitro. In addition, once an interaction between two proteins has been detected, the mammalian two-hybrid assay can be used with deletions or site-directed mutagenesis to identify domains and amino acids involved in specific protein-protein interactions.

Acknowledgments

The authors thank Ning Jiang, Xu Li, Lisa Hexdall, Katherine Felts, Chao-Feng Zheng, Peter Vaillancourt, Carsten Carstens, Alan Greener, Mary Buchanan, and John Bauer of the Genetic Systems group at Stratagene for reagents, discussions, suggestions, and excellent jokes.

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