Navigation Links
New Mammalian Two-Hybrid System Detects Protein-Protein Interactions

than interactions observed in a foreign environment. The confirmation eliminates the possibility of a false positive that is an artifact from working in yeast cells or in vitro. In addition, once an interaction between two proteins has been detected, the mammalian two-hybrid assay can be used with deletions or site-directed mutagenesis to identify domains and amino acids involved in specific protein-protein interactions.

Acknowledgments

The authors thank Ning Jiang, Xu Li, Lisa Hexdall, Katherine Felts, Chao-Feng Zheng, Peter Vaillancourt, Carsten Carstens, Alan Greener, Mary Buchanan, and John Bauer of the Genetic Systems group at Stratagene for reagents, discussions, suggestions, and excellent jokes.

REFERENCES
  1. Phizicky, E.M. and Fields, S. (1995) Microbiological Review 59: 94-123.

  2. Fields, S. and Song, O.-K. (1989) Nature (London) 340: 245-246.

  3. Chien, C.-T., et al. (1991) Proc. Natl. Acad. Sci. USA 88: 9578-9582.

  4. Fields, S. and Sterngianz, R. (1994) Trends Genetics 10: 286-292.

  5. Osborne, M.A., et al. (1997) In P.L Bartel and S. Fields (Eds.) The Yeast Two-Hybrid System. Oxford University Press. New York, New York.

  6. Buckholz, R.G. and Gleeson, M.A.G. (1991) Biotechnology 9: 1067.

  7. Bartel, P.L. and Fields, S. (1995) Methods in Enzymology 254: 241-263.

  8. Dang, C.V., et al. (1991) Mol. Cell. Biol. 11: 954-962.

  9. Schmitz, M.L. and Baeurle, P.A. (1991) EMBO J. 10: 3805-3817.

  10. Rivera, V.M. (1998) Methods: A Companion to Methods in Enzymology 14: 421-429.

  11. Shocke
    '"/>



Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
2. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
3. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
4. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
5. A New Lambda Vector for Mammalian Expression
6. Mammalian and Bacterial Expression in One Vector
7. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
8. Mammalian Expression Vectors with Interchangeable Selectable Markers
9. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
10. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
11. Reagents for Up and Down-Regulation of miRNA Activity in Mammalian Cells
Post Your Comments:
(Date:7/30/2015)... over 60,000 customers across the globe, ISN improves the efficiency and effectiveness of contractor ... 60,000 safe and reliable contractors in over 80 countries. ... ... Over 60,000 Worldwide Customers In More Than ... Headquartered in Dallas, TX , ISN has additional offices in ...
(Date:7/30/2015)... ... July 30, 2015 , ... ... fluid applications and designed for continuous operation up to 1500 bar. The sanitary ... safety, efficiency, and preventing toxic contamination. , The Pony™ NS2006L homogenizer is an ...
(Date:7/29/2015)... (Q2 2015: +14% CER / 20% of sales) led the regional performance ... , as well as solid contributions from Korea, India ... Middle East / Africa (Q2 ... , Turkey and the United Kingdom ... 11%, excluding U.S. HPV sales, on demand across all customer classes. The top ...
(Date:7/29/2015)... Research and Markets ... the "Global Biosimilars Market, 2015 - 2025" ... Global Biosimilars Market, 2015 - 2025, report provides ... market. With the blockbuster biologics losing patent protection ... are being viewed as viable substitutes to the ...
Breaking Biology Technology:ISN Reaches Over 60,000 Worldwide Customers 2ISN Reaches Over 60,000 Worldwide Customers 3GEA Announces the Pony™ NS2006L… a Self-contained, High Pressure Lab Homogenizer for Product Development Ensuring Maximum Efficiency and Safety 2QIAGEN Reports Results for Second Quarter and First Half of 2015 2QIAGEN Reports Results for Second Quarter and First Half of 2015 3QIAGEN Reports Results for Second Quarter and First Half of 2015 4QIAGEN Reports Results for Second Quarter and First Half of 2015 5QIAGEN Reports Results for Second Quarter and First Half of 2015 6QIAGEN Reports Results for Second Quarter and First Half of 2015 7QIAGEN Reports Results for Second Quarter and First Half of 2015 8QIAGEN Reports Results for Second Quarter and First Half of 2015 9QIAGEN Reports Results for Second Quarter and First Half of 2015 10Global Biosimilars Market to 2025: Monoclonal Antibodies (mAbs), Insulin, Interferons, G-CSF/ GM-CSF, Erythropoietins and Others 2Global Biosimilars Market to 2025: Monoclonal Antibodies (mAbs), Insulin, Interferons, G-CSF/ GM-CSF, Erythropoietins and Others 3
... the most important frontiers in medicine. Although no significant achievements for curing ... terms of developing such approaches. By the year 2015, global market for ... ... CA (PRWEB) November 4, 2008 -- Advancements in gene therapy have provided ...
... When Albrecht Durer and other Renaissance artists painstakingly etched ... and transferred the images to paper with a press, ... same technique would uncover the secrets of human cells. ... have borrowed a technique from such "intaglio" printing to ...
... VALENCIA, Calif., Nov. 3 MannKind Corporation,(Nasdaq: MNKD ... and cancer, announced today that it will present at,the ... 10, 2008 at 9:55 AM (EST) at the New ... can access a link to the live web cast ...
Cached Biology Technology:World Gene Therapy Market to Reach $484 Million by 2015, According to New Report by Global Industry Analysts, Inc. 2World Gene Therapy Market to Reach $484 Million by 2015, According to New Report by Global Industry Analysts, Inc. 3Protein-printing technique gives snapshots of immune system defense 2Protein-printing technique gives snapshots of immune system defense 3
(Date:7/21/2015)... 21, 2015  NXT-ID, Inc. (NASDAQ: NXTD ... company focused on the growing mobile commerce market ... that it has filed provisional patent 62/192218 for ... This invention highlights next generation payment ... account, but also the user using unique personal ...
(Date:7/13/2015)... Conn. , Jul. 13, 2015 NXT-ID, ... "Company"), a biometric authentication company focused on the ... has filed provisional patent 62/188684 for ... achieves another new convenient and secure method to ... new, innovative payment methods, introduced with its groundbreaking ...
(Date:7/9/2015)... , July 9, 2015  Synaptics Inc. ... of human interface solutions, today announced a ... first fully hardware encapsulated fingerprint sensor and ... technology is literally off the grid, isolating ... matching within the fingerprint sensor to provide ...
Breaking Biology News(10 mins):NXT-ID Patents Personalized Tokenized Payments for Next Generation Secure Payment Technology 2NXT-ID Patents Personalized Tokenized Payments for Next Generation Secure Payment Technology 3NXT-ID Patents Personalized Tokenized Payments for Next Generation Secure Payment Technology 4NXT-ID Patents Behavior-Directed Payments 2NXT-ID Patents Behavior-Directed Payments 3NXT-ID Patents Behavior-Directed Payments 4Synaptics Announces World's First Match-in-Sensor Fingerprint Authentication Technology 2Synaptics Announces World's First Match-in-Sensor Fingerprint Authentication Technology 3
... (CHICAGO) Women with the BRCA1 or BRCA2 ... risk of breast and ovarian cancer, can safely take ... removal of their ovaries, according to new research from ... Pennsylvania which will be presented Monday, June 6 during ...
... in partnership with academic publishers Pensoft, creates so-called ... species observations, specimen collections and other biodiversity data. ... version of GBIF,s Integrated Publishing Toolkit (IPT 2.0.2) ... information about a dataset accessible via the GBIF ...
... that the critically short supply of livers for organ ... with a substance that protects them from damage after ... in ACS, journal Molecular Pharmaceutics . Ram ... transplants has grown over the years, though the number ...
Cached Biology News:Women with BRCA mutations can take hormone-replacement therapy safely after ovary removal 2Women with BRCA mutations can take hormone-replacement therapy safely after ovary removal 3New incentive for biodiversity data publishing 2
... aptamer screening and binding optimization and built ... on-chip synthesis platform. These microarrays are available ... Microarray Service. Probe Content ... greater than 1500 known aptamer sequences. Add ...
... comprehensive service for custom peptide microarray ... synthesized on Paraflo microfluidic chips for ... serum samples. This comprehensive service includes ... assays using sample(s) provided by you, ...
... screening and binding optimization and built on ... synthesis platform. Thousands of customer specified sequences ... can provide assistance with custom sequence design. ... our comprehensive DNA/RNA Aptamer Microarray Service. ...
... commonly used as a fusion partner when ... GSTTag sequence has been reported to enhance ... solubility of its fusion partners. When expressed ... fusion proteins can be purified with immobilized ...
Biology Products: