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New Mammalian Two-Hybrid System Detects Protein-Protein Interactions

than interactions observed in a foreign environment. The confirmation eliminates the possibility of a false positive that is an artifact from working in yeast cells or in vitro. In addition, once an interaction between two proteins has been detected, the mammalian two-hybrid assay can be used with deletions or site-directed mutagenesis to identify domains and amino acids involved in specific protein-protein interactions.

Acknowledgments

The authors thank Ning Jiang, Xu Li, Lisa Hexdall, Katherine Felts, Chao-Feng Zheng, Peter Vaillancourt, Carsten Carstens, Alan Greener, Mary Buchanan, and John Bauer of the Genetic Systems group at Stratagene for reagents, discussions, suggestions, and excellent jokes.

REFERENCES

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  4. Fields, S. and Sterngianz, R. (1994) Trends Genetics 10: 286-292.

  5. Osborne, M.A., et al. (1997) In P.L Bartel and S. Fields (Eds.) The Yeast Two-Hybrid System. Oxford University Press. New York, New York.

  6. Buckholz, R.G. and Gleeson, M.A.G. (1991) Biotechnology 9: 1067.

  7. Bartel, P.L. and Fields, S. (1995) Methods in Enzymology 254: 241-263.

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  11. Shocke
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