Navigation Links
New Mammalian Two-Hybrid System Detects Protein-Protein Interactions


A powerful method to detect protein interactions in mammalian cells

Tanya Hosfield Cathy Chang
Stratagene

With Stratagenes mammalian two-hybrid assay kit, hybrid genes are used to detect protein-protein interactions via the activation of reporter gene expression. This expression occurs as a result of reconstitution of a functional transcription factor caused by the association of two hybrid proteins. It allows researchers to study interactions in vivo between mammalian proteins that require posttranslational modification or external stimulation not present in lower eukaryotes.

Protein-protein interactions in mammalian cells are the basis of virtually every cellular process, such as DNA replication, transcription, translation, splicing, secretion, cell cycle control, signal transductions, and intermediate metabolism.1 As efforts to sequence whole genomes result in data for vast numbers of new proteins, there is a corresponding need to identify interactions among these proteins. Methods used to discover these interactions will be extremely helpful in elucidating the in vivo function of newly discovered genes and, in turn, will aid in further understanding known protein functions.

One of the most powerful methods for studying protein-protein interactions is the yeast two-hybrid system developed by Dr. Stanley Fields and colleagues.2 This system has been highly useful for detecting and identifying protein-protein interactions in vivo. The Fields two-hybrid system, and the many second-generation versions currently used,2,3,4,5 exploit the modular nature of a transcriptional activator. Transcriptional activators, such as the GAL4 protein of yeast, contain a DNA binding domain and an activation domain that can be separated. If these domains are then reconstituted in trans, their acti
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
2. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
3. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
4. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
5. A New Lambda Vector for Mammalian Expression
6. Mammalian and Bacterial Expression in One Vector
7. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
8. Mammalian Expression Vectors with Interchangeable Selectable Markers
9. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
10. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
11. Reagents for Up and Down-Regulation of miRNA Activity in Mammalian Cells
Post Your Comments:
(Date:12/19/2014)... Egenix, Inc. is pleased to announce that Lionel Goldfrank ... succeed Donald Fresne ; Mr. Fresne has also resigned ... of the Board.  In recognition of the many contributions and ... twenty years, the Board designated Mr. Fresne as Chairman Emeritus. ... Search Committee to find a suitable replacement with Biotech industry ...
(Date:12/17/2014)... (PRWEB) December 17, 2014 Based ... the overall surgical imaging market is dominated by ... the surgical imaging market. These players include GE ... Ziehm Imaging (Germany). , Full Report Copy ... of GE Healthcare can be attributed to its ...
(Date:12/17/2014)... Once more, EMAAR has selected BioShaft ... water treatment plant to phase one of BayLaSun, a ... years ago Bioshaft successfully supplied and operated a packaged ... towers with an occupancy of 900 residences. The ... per day and was signed on September 16th, 2014, ...
(Date:12/17/2014)... CA (PRWEB) December 17, 2014 Audacity, ... activation agency and member of the Huntsworth Health Group, ... to its executive team. In her role, Gonzales will ... , “We are incredibly honored to welcome a talent ... Chief and CEO, Gaëtan Fraikin. “She is a proven ...
Breaking Biology Technology:Global Refurbished Surgical Imaging Market by Product & Application is Estimated to Reach $1.25 Billion by 2019 - New Research Report by MarketsandMarkets 2Global Refurbished Surgical Imaging Market by Product & Application is Estimated to Reach $1.25 Billion by 2019 - New Research Report by MarketsandMarkets 3Global Refurbished Surgical Imaging Market by Product & Application is Estimated to Reach $1.25 Billion by 2019 - New Research Report by MarketsandMarkets 4Audacity Welcomes Healthcare Communications Expert Jamie Gonzales as Senior Vice President of Client Services 2
... MUNDELEIN, Ill., June 2 Medline Industries, Inc., ... medical,supplies, announced today that it has signed a ... a manufacturer of liquid products,for personal care, infection ... recent shortages of mouth rinse in the healthcare ...
... BioSpace, the world,s leading,online bioscience job ... revealed,this morning the 2008 BioCapital(TM) Hotbed Campaign ... and Washington, D.C., (Photo: http://www.newscom.com/cgi-bin/prnh/20080602/CLM086 ... companies and academic institutions from,the biotechnology, pharmaceutical ...
... CLEVELAND, Ohio, June 2 Simbionix has announced ... its LAP Mentor simulator that,gives trainees hands-on experience ... of ectopic pregnancy, tubal,sterilization and oophorectomy., The ... suite of laparoscopic,procedures developed for the LAP Mentor, ...
Cached Biology Technology:Medline Signs 3-Year Purchasing Agreement for Mouth Rinse From Chester Labs 2BioSpace Releases the 2008 BioCapital(TM) Hotbed Campaign 2Simbionix Takes Non-Invasive GYN Surgical Training to the Next Level 2
(Date:12/19/2014)... 18, 2014 Research and Markets ( ... "Micro Market Monitor: North America Perimeter Security Systems Market" ... http://photos.prnewswire.com/prnh/20130307/600769 The North American perimeter ... of 3.6% from 2014 to 2019. Although the U.S. ... Canada is expected to grow at ...
(Date:12/17/2014)... NEW YORK , Dec. 15, 2014 /PRNewswire-USNewswire/ ... a Good Clinical Practices (GCP) audit to confirm ... Administration (FDA) regulations. This accomplishment enables HITLAB to ... trials utilizing the highest principles for patient safety ... to improve global healthcare access, quality, and delivery ...
(Date:12/11/2014)... 09, 2014 Research and Markets ... ( http://www.researchandmarkets.com/research/twhjll/biometrics_market ) has announced the addition ... Region 2015-2019"  report to their offering. ... is advances in technology. With continuous advances in ... to the latest standard that meets the needs ...
Breaking Biology News(10 mins):Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 3HITLAB Completes GCP Audit for FDA Smart Device & App Compliance 2Biometrics Market in the APAC Region 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2
... brassinolide, plants "pump up" like major league baseball players ... cell's nucleus, researchers at the Salk Institute for Biological ... job at the molecular level. , The Salk ... Plant Molecular and Cellular Biology Laboratory and a Howard ...
... plant steroid brassinolide, plants "pump up" like major league ... into the cell's nucleus, researchers at the Salk Institute ... accomplishes its job at the molecular level. , ... in the Plant Molecular and Cellular Biology Laboratory and ...
... foods to cause allergic reactions in humans is a ... are in place to ask questions about the allergy-causing ... answers. , But all of that could change as ... animal model to test whether genetically engineered foods could ...
Cached Biology News:Ozone recovering, but unlikely to stabilize at pre-1980 levels, says study 2Ozone recovering, but unlikely to stabilize at pre-1980 levels, says study 3Salk scientists untangle steroid hormone signaling in plants 2Salk scientists untangle steroid hormone signaling in plants 3Tests may help answer questions about GMOs and allergies 2
BD BioCoat Osteologic Coverslips that have been sterilized by ETO gas. Coating : Osteologic&trade ,Thickness : 0.16 mm ,Diameter : 12.7 mm ,Gridded : No ...
...
... bis (3-ethylbenzthiazoline-6-sulfonic Acid) Clear to very ... and H 2 O 2 in a ... substrate that is soluble, safe, convenient and has ... develops intense blue-green color that can be measured ...
...
Biology Products: