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New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence ,,, Humanized Renilla GFP Reporter

N-terminus of the protein of interest. Inserts into the phrGFP-C vector are expressed as C-terminal fusions to hrGFP.

We tested the protein fusion vectors by fusing hrGFP to a series of genes that encode subcellularly localized proteins. In Figure 3, each fluorescent microscope photograph shows hrGFP expression localized to the organelle that corresponds to the appropriate fusion protein. These data confirm that the phrGFP-N1 and phrGFP-C protein fusion vectors provide convenient plasmid construction and high-level fluorescence of hrGFP fusion proteins within the cell. In addition, each of the four subcellular localization vectors shown in Figure 3 are available as stand alone products. These vectors allow real-time monitoring of the fate of peroxisomes, mitochondria, golgi and nuclei in both transient and stable applications.

Fig.3

Useful hrGFP-Based Controls for Retroviral Expression Systems

Fig.4

Along with mammalian expression vectors, the hrGFP gene is used in the pFB-hrGFP retroviral vector (Figure 4) as a reporter vector for determining the efficiency of retroviral transductions where expression cannot be determined directly. Transduction efficiency and gene expression of the pFB or pFB-neo retroviral vectors containing genes of interest can be confirmed indirectly, by conducting an additional transduction experiment of the reporter pFB-hrGFP in parallel. This vector is ideal for use with Stratagenes ViraPort retroviral expression system as a tool to verify transduction efficiency.8

The pFB-hrGFP retroviral reporter vector is availabl
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