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New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence ,,, Humanized Renilla GFP Reporter



New Vitality hrGFP expression vectors for monitoring gene expression and localizing proteins in vivo

New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter

Brenda Rogers Keith Chen Katherine Felts

Stratagene

Stratagene has added new vectors to the Vitality hrGFP mammalian expression vector product line Y. The new Vitality phrGFP-C and phrGFP-N1 protein fusion vectors, like the original Vitality pIRES-hrGFP-1a and pIRES-hrGFP-2a bicistronic expression vectors , are used for monitoring gene expression and protein localization. The Vitality vectors encode the humanized Renilla reniformis green fluorescent protein (hrGFP), a reporter protein known for its low cellular toxicity. Expression studies with the hrGFP reporter maintain a healthy cell population and ensure that results are biologically valid. Related products include the Vitality pFB-hrGFP retroviral vector and transduction-ready retroviral supernatants, useful controls for retroviral gene expression systems, and a variety of subcellular localization vectors. With this array of hrGFP-based products, expression of your gene of interest can be optimized for your specific applications. Here, we confirm the utility and versatility of Vitality vectors, illustrated in a variety of expression studies.

The green fluorescent protein (GFP) gene from the sea pansy Renilla reniformis has
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