( mination reagents with the novelDNA-...)New Insights Into DNA Decontamination

One surprising result was the finding that some of the conventional reagents only use the principle of modification or denaturation for the inactivation of DNA molecules. This was determined in the test by the complete absence of any degraded DNA molecule. The genetic information encoded in these DNA strands is only masked but not destroyed. By chemical demasking reactions the genetic information of these molecules would be available again and could also be amplified by enzymatic reactions. Based on our knowledge about gene technology and the principles of recombination, we concluded that these reagents are no longer sufficient.

But even reagents that show degradation of DNA cause only limited and partial destructions. Hence, very large DNA fragments which contain the complete genetic information, still survive treatment (Fig. 1). Only our novel DNA-ExitusPlus achieves rapid and efficient degradation. By spraying DNA-ExitusPlus on surfaces in the lab complete decontamination will be achieved, when the whole surface, especially the critical points, are wetted. To remove heavy contaminations, we recommend to repeat the treament with DNAExitusPlus.

The strand breaking activity of DNA-ExitusPlus is independent of the size and sequence of the DNA fragments since the destruction is based on a chemical action and not an enzymatic activity. Large plasmids require a longer incubation time than small ones (e.g. a primer). Assuming that a theoretical nicking activity of 100,000 nicks per minute is present, all DNA fragments will be destroyed, independent of their size. Smaller fragments will disappear before the larger ones. Applying this theory to a test molecule (ccc form, 6 kb plasmid) onl
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