Navigation Links
New Insights Into DNA Decontamination

Dr. Karl-Heinz Esser, multiBIND biotec GmbH,
Dr. Wolfram H. Marx, AppliChem GmbH and
Prof. Dr. Thomas Lisowsky, multiBIND biotec GmbH



Advanced experiments in gene technology demonstrate that even small amounts of free DNA molecules are sufficient to cause infections, recombinations or biological transformations [1,2]. Additionally, the complete decontamination of equipment and surfaces from any DNA molecules is important for biological containment, safety and prevention of artifacts in PCR amplification experiments. Using new methods available to detect extremely low levels of DNA molecules, we investigated the molecular mechanism of action of various commercially available DNA decontamination reagents. When tests were performed under extreme conditions, i.e. high concentrations of DNA and short incubations times, two major problems were apparent: First, none of the conventional reagents destroyed DNA molecules efficiently and second, all reagents contained components with corrosive or even toxic properties. Consequently, we saw the need to develop new solutions for effective DNA decontamination. Now, we present the latest developments and assays comparing the new AppliChem DNA decontamination reagent, DNA-ExitusPlus, with other conventional products. DNA-ExitusPlus guarantees fast and efficient destruction of nucleic acids without corrosive or toxic properties.

DNA decontamination reagents use three different molecular principles for destruction or inactivation of genetic material: modification, denaturation and degradation. Depending on the composition of the reagents, the different mechanisms may be combined. Safe DNA decontamination depends on the degradation of DNA into very small fragments. We developed a DNA degradation test to compare conventional deconta
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Decontamination and inhibition of ribonucleases
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New Insights Into DNA Decontamination

(Date:8/22/2014)... (PRWEB) August 22, 2014 The AMA ... and its members in Alaska that Alaska Governor Sean ... Resolution 15, further defining public use of Unmanned Aircraft ... Task Force. House Bill 255states that it is, “An ... images captured by an unmanned aircraft system.” It defines ...
(Date:8/22/2014)... Toronto, Canada (PRWEB) August 22, 2014 ... traits in model organisms can be accelerated by ... design and manufacturing technologies, the custom SeqCap EZ ... such research. , Mr. Watson will additionally present ... seen with commercially available human and mouse exome ...
(Date:8/21/2014)... 2014  BioSpecifics Technologies Corp. (NASDAQ: ... class collagenase-based products marketed as XIAFLEX ® ... and XIAPEX ® in the EU, today ... double-blind Phase 2a study of CCH for the ... The results showed that all three doses of ...
(Date:8/21/2014)... at the Institute for Molecular Medicine Finland (FIMM), ... developed a novel "man and machine" decision support ... aid was described in PLOS One ... based on computer vision algorithms similar to those ... of only the diagnostically most relevant areas. Tablet ...
Breaking Biology Technology:State of Alaska Signs Unmanned Aircraft Systems Bill 2Efficient Identification of Protein-Coding Variants in a Model Organism Through Exome Sequencing, New Webinar Hosted by Xtalks 2BioSpecifics Technologies Corp. Announces Positive Data from Phase 2a Study of CCH for Treatment of Cellulite 2BioSpecifics Technologies Corp. Announces Positive Data from Phase 2a Study of CCH for Treatment of Cellulite 3BioSpecifics Technologies Corp. Announces Positive Data from Phase 2a Study of CCH for Treatment of Cellulite 4BioSpecifics Technologies Corp. Announces Positive Data from Phase 2a Study of CCH for Treatment of Cellulite 5A novel 'man and machine' decision support system makes malaria diagnostics more effective 2
... stents after opening clogged arteries has led a team of ... in Milwaukee to suggest that stents designed with thinner ... generation of stents. Their findings are published in the July ... , ,One of the most common methods for treating heart ...
... medical profession, one of the greatest obstacles in taking ... that naturally accompanies treatment. Patient histories and care instructions ... if multiple hospitals are involved, more levels of complication ... all this information seems like a Holy Grail, a ...
... of technological innovation sometimes starts out-of-control wildfires of politics, ... IT shop. If youre lucky, youve got some folks ... rapidly shifted from their regular duties to handle the ... reputations are more common results. If youre particularly unlucky, ...
Cached Biology Technology:Medical College discovery leads to new stents 2Human factors and IT: Designing for patient safety 2Human factors and IT: Designing for patient safety 3IT wildfires 2IT wildfires 3
(Date:8/21/2014)... including researchers from the University of Georgia recently published the ... journal Science . Their discovery paves the way for ... farming and industry. , Canola is grown across much ... is increasingly cultivated in Georgia. Canola oil used for cooking ... and rich supply of omega-3 fatty acids, but the plant ...
(Date:8/21/2014)... -- Nxt-ID, Inc. (OTCQB: NXTD), a biometric authentication company ... that its shares of common stock and the warrants ... proposed underwritten public offering of common stock and warrants ... Market, subject to closing of its proposed underwritten public ... trade under the symbol "NXTD" and "NXTDW," respectively. ...
(Date:8/21/2014)... and other itchy, flaky maladies in humans has now ... reefs and the extreme environments of arctic soils and ... journal PLOS Pathogens considers the diversity, ecology, ... Malassezia in light of new insights gained from ... University of Hawai,i at Mānoa scientist Anthony Amend discovered ...
Breaking Biology News(10 mins):Canola genome sequence reveals evolutionary 'love triangle' 2Nxt-ID Approved for NASDAQ Capital Market Listing 2Nxt-ID Approved for NASDAQ Capital Market Listing 3From dandruff to deep sea vents, an ecologically hyper-diverse fungus 2
... Mathematical models have become invaluable decision-making tools for ... Kingdom's foot-and-mouth epidemic of 2001, models can be ... underlying characteristics of an infection and they can ... however, such models make implicit assumptions that may ...
... pandas in California or kangaroos in Argentina. , For David ... a professor of integrative biology at the University of California, ... of a type of salamander that comprises the majority of ... and rare outside the Americas. , "I've discovered and named ...
... have solved the structure of a crucial human immune ... three. In an upcoming issue of the journal Science, ... composed of 23 leucine-rich repeats (LRRs). , The structure ... before, an essential step toward fully understanding the critical ...
Cached Biology News:Understanding biases in epidemic models important when making public health predictions 2Discovery of an American salamander where it shouldn't be: Korea 2Discovery of an American salamander where it shouldn't be: Korea 3Discovery of an American salamander where it shouldn't be: Korea 4Scripps research scientists solve structure of a critical innate immune system protein 2Scripps research scientists solve structure of a critical innate immune system protein 3Scripps research scientists solve structure of a critical innate immune system protein 4
Bethyl Laboratories packages antibodies, conjugates and calibrators to provide quantitative ELISA kits. Each kit contains the following components, sufficient for 1000 single well assays....
Bethyl Laboratories packages antibodies, conjugates and calibrators to provide quantitative ELISA kits. Each kit contains the following components, sufficient for 1000 single well assays....
Request Info...
... and IHC Protocol Development ,Antibody Titer ... in Animal or Human Cells and ... and Tissues ,Peroxidase, Alkaline Phosphatase, Immunogold, ... Electron Microscopy ,Protein Localization in Transgenic ...
Biology Products: