Navigation Links
New Fusion Trans-Activator Plasmids for Studying Signal Transduction,,,Pathways

Ras-responsive.11 The pFA-cFos plasmid was constructed by inserting the DNA fragment encoding residues 208 to 313 of rat c-Fos12 into the BamH I and EcoR I sites of pFA-CMV. Our results (figure 3A) in transient transfection assays demonstrate that the MEKK protein activates the GAL4-c-Fos fusion protein expressed from the pFA-cFos plasmid. The mechanism of this activation is not yet characterized. PKA, MEK1 and other extracellular stimuli failed to activate the GAL4-c-Fos fusion protein (data not shown).

figure 3B

ATF2, or activating transcription factor 2, belongs to a large family of transcription factors that recognize the ATF2/CRE elements identified in the adenovirus early gene promoters.8-10,13 ATF2 forms homodimers as well as heterodimers with other members of the ATF, Jun and Fos transcription factors. The regulation of ATF2 is not fully characterized. However, phosphorylation of Thr-69 and Thr-81 in ATF2 by JNK is known to activate the ATF2 transcription factor.13 In order to construct pFA-ATF2 (1-96), the DNA fragment encoding amino acid residues 1-96 of human ATF214,15 was inserted into the BamH I and EcoR I sites of the pFA-CMV vector. Consistent with previous reports, the GAL4-ATF2 fusion protein expressed from the pFA-ATF2 plasmid was strongly activated by the MEKK protein in transient transfection assays (figure 3A).

Conclusions

Stratagene has constructed three new fusion trans-activator plasmids for use with the PathDetect in vivo signal transduction pathway reporting systems. All three of these plasmids can be used in a variety of mammalian cell lin
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. Stable HeLa Luciferase Reporter Cell Lines Expressing GAL4 Fusion Transactivators
3. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
4. Comparison of Performance between the AlphaQuest - HTS and the Fusion α Multilabel Reader for Detection of cAMP
5. Mobile Phase Optimization for the Analysis of an Antibody-Based Fusion Protein and Aggregates using a TSKgel Super SW3000 Size-Exclusion Column
6. New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways
7. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
8. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
9. Enzyme Immunoassay for Studying Intracellular Levels of cAMP
10. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
11. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
Post Your Comments:
(Date:7/10/2014)... quantum Hall effect in bilayer graphene and shown that ... an electric field. , The fractional quantum Hall ... sheets are exposed to large magnetic fields, is a ... electrons behave as a single system. However, while the ... details of this collective behavior remain not well understood, ...
(Date:7/10/2014)... recent study by researchers at the University of ... physical mechanisms governing the interplay of spin and ... limits of ultrafast spintronic devices for data storage ... charge as well as spin-angular momentum. In a ... there is no spin current," explained David Cahill, ...
(Date:1/15/2014)... , Jan. 15, 2014  Bill Jacobs Automotive, a group of ... teaming up with the Heartland Blood Center and offering free ... The drive takes place Saturday, Jan. 18 at the Bill ... p.m. (Photo: http://photos.prnewswire.com/prnh/20140115/MN46637 )  The ...
(Date:1/15/2014)... Cynvenio, a cancer diagnostics company focused ... analysis of tumor cells in the bloodstream, today announced ... decision support application powered by CollabRx, Inc. , ... test, Therapy Finder will provide breast cancer patients and ...
Breaking Biology Technology:Columbia researchers observe tunable quantum behavior in bilayer graphene 2Columbia researchers observe tunable quantum behavior in bilayer graphene 3University of Illinois study advances limits for ultrafast nano-devices 2Join the Bill Jacobs Auto blood drive and receive a free oil change 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 2Cynvenio Offers New Breast Cancer Therapy Finder Application for its ClearID Breast Cancer Genomic Test 3
... Accera, Inc., a biotech company specializing in ... to the Alzheimer,s Foundation of America (AFA) for sponsorship ... Alzheimer,s Disease Awareness Month.  These funds will be used ... individuals with Alzheimer,s disease (AD) and their families, especially ...
... SHELTON, Conn., Oct. 24, 2011 Modern Plastics, one ... shapes and medical-grade plastics, has become ISO 13485-2003 certified ... Tube, Film and Resin. "This is ... customer commitment to service and quality," said Bing J. ...
... Amsterdam Molecular Therapeutics (Euronext: AMT), a leader in ... the European Medicines Agency,s (EMA) Committee for Medicinal Products ... that Glybera (alipogene tiparvovec) is not approvable at this ... recommendation of CHMP Rapporteurs, the Scientific Advisory Group (SAG), ...
Cached Biology Technology:Accera, Inc. Donates $50,000 to the Alzheimer's Foundation of America for National Memory Screening Day 2Accera, Inc. Donates $50,000 to the Alzheimer's Foundation of America for National Memory Screening Day 3Amsterdam Molecular Therapeutics Receives Opinion on Re-examination of Glybera® Marketing Authorisation Application 2Amsterdam Molecular Therapeutics Receives Opinion on Re-examination of Glybera® Marketing Authorisation Application 3
(Date:7/10/2014)... COMMACK, N.Y. , July 2, 2014 ... Inc. (ABD) to announce the appointment of the world-renowned ... its Board of Directors. Dr. Ginsburg, with ... two facilities: NY Presbyterian Hospital and Good Samaritan Regional ... Breathing Pacemaker since the late 1980,s and is considered ...
(Date:7/10/2014)... -- Research and Markets ( http://www.researchandmarkets.com/research/h7h8lq/gesture ) has ... Recognition & Touch-Less Sensing (2D, 3D, Ultrasonic, IR, ... to their offering http://photos.prnewswire.com/prnh/20130307/600769 ... Sensing Market to Grow Exponentially. Touch-less sensing applications ... companies were unable to leverage this technology due ...
(Date:7/10/2014)... 2014 Fingerprint Cards, (FPC,s) ... win (DW). An Asian OEM has selected FPC1021 for a ... in August 2014.   FPC is proud to ... a planned date for start of mass production in August ... SEK 5M, for delivery in the first half of July. ...
Breaking Biology News(10 mins):Avery Biomedical Devices, Inc. announces the appointment of Mark E Ginsburg, M.D. to its Board of Directors 2Global Gesture Recognition & Touch-Less Sensing (2D, 3D, Ultrasonic, IR, Capacitive) Market - Forecasts to 2020 2FPC Wins DW and Initial Ramp Order for FPC1021 Touch Sensor for Flagship Smartphone from Asian OEM 2
... This release is available in German . ... or malaria treatment could soon become easier - thanks to ... Institute of Molecular Physiology in Dortmund aim to facilitate the ... Scaffold Hunter, acts as a tool for navigating chemical space. ...
... Palo Alto, CAWith the information explosion, it,s ... interactions that proteins have with each other and ... interactive, so-called membrane proteins regulate nutrients and water ... interface with neighboring cells and within the cell. ...
... generates electricity can also remove 90 percent of salt from ... researchers from China and the U.S. Clean water for ... some parts of the world. Its availability in the future ... using either a reverse osmosis process -- one that pushes ...
Cached Biology News:Navigating in the ocean of molecules 2Navigating in the ocean of molecules 3Carnegie donates landmark clones to biology 2Wastewater produces electricity and desalinates water 2Wastewater produces electricity and desalinates water 3
... E-PAGE Gels easier and more efficient by ... several types of pre-cut pre-assembled membrane/filter paper ... 13.5 cm E-PAGE Gels*. The available nitrocellulose ... western transfer of proteins (Figure 1 Table ...
... carbohydrate analysis standard is a lyophilized ... sugar alcohol; molecular weights range from ... provided for use with the Aminex ... kit for column testing or semiquantitative ...
... kit was tested on rat tissue extracts (liver, ... ( S. cerevisae ) extract. It was also ... and cell lines. Application: A simple and reliable ... based on the detection of the inorganic phosphate ...
... is the condition in which a lack ... and the various antioxidants. Oxidative stress ... of several diseases, including artherosclerosis, chronic inflammatory ... several antioxidant systems that are very important ...
Biology Products: