Navigation Links
New Fusion Trans-Activator Plasmids for Studying Signal Transduction,,,Pathways

Study the signal transduction pathway of your choice

Li Xu Chao-Feng Zheng
Stratagene Cloning Systems, Inc.

The PathDetect in vivo signal transduction pathway reporting systems are designed for assessing the in vivo activation of signal transduction pathways. The PathDetect trans-reporting systems include a unique fusion trans-activator plasmid that expresses pathway-specific trans-activator proteins fused to the yeast GAL4 DNA binding domain. To facilitate the construction and expression of new fusion trans-activator proteins, Stratagene has designed the new pFA-CMV plasmid for expressing the activation domain of any transcription factor fused to the yeast GAL4 DNA binding domain. The pFA-CMV plasmid features a multiple cloning site and the cytomegalovirus (CMV) promoter for convenient construction and high-level expression of fusion trans-activator proteins in a variety of mammalian cell lines. We describe two new fusion trans-activator plasmids (constructed with the pFA-CMV plasmid) expressing GAL4 fusions with c-Fos (208-313) and ATF2 (1-96).

figure 1

Recently, Stratagene introduced the PathDetect in vivo signal transduction pathway trans-reporting systems, which are designed for specific, rapid and convenient assessment of the in vivo activation of signal transduction pathways.1 These reporting systems are used for studying the in vivo effects of new genes, growth factors, drug candidates and extracellular stimuli on the activation of c-Jun N-terminal kinase (JNK),2,3 mitogen-activated protein kinase (MAPK),4,5 cyclic AMP-dependent protein (PKA)6 and other signaling molecules leading to the activation of these kinases. The PathDetect report
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. Stable HeLa Luciferase Reporter Cell Lines Expressing GAL4 Fusion Transactivators
3. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
4. Comparison of Performance between the AlphaQuest - HTS and the Fusion α Multilabel Reader for Detection of cAMP
5. Mobile Phase Optimization for the Analysis of an Antibody-Based Fusion Protein and Aggregates using a TSKgel Super SW3000 Size-Exclusion Column
6. New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways
7. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
8. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
9. Enzyme Immunoassay for Studying Intracellular Levels of cAMP
10. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
11. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
Post Your Comments:
(Date:1/14/2014)... 2014 Date: Friday, April 11, 2014 , ... Club, 1360 Almshouse Road, Warrington, Pa. , Details: ... dedicated to finding a cure for hepatitis B and improving ... its annual Crystal Ball on Friday, April 11 at Warrington ...
(Date:1/14/2014)... New York, NY (PRWEB) January 14, 2014 ... coverage of Alliqua, Inc. (OTCQB: ALQA). Alliqua is an ... products to serve the wound care market. , Free ... Alliqua was restructured with a seasoned management team and ...
(Date:1/14/2014)... 14, 2014  RXi Pharmaceuticals Corporation (OTCQX: RXII), a ... innovative therapies addressing major unmet medical needs using ... Notice of Allowance from the United States Patent ... compounds (sd-rxRNA®), for the treatment of fibrosis. The ...
(Date:1/14/2014)... 14, 2014 During the 1600’s through the ... “The Doctor’s Plague.” In this time period, doctors did not ... at times, to the death of vulnerable patients. In the ... that they may be unwittingly transmitting herpes viruses to their ...
Breaking Biology Technology:Hepatitis B Foundation to Host Annual Crystal Ball Gala 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 3RXi Pharmaceuticals Receives US Notice of Allowance for a Key Patent Relating to its Self-Delivering Technology with sd-rxRNAs targeting CTGF, including RXI-109, for the Treatment of Fibrotic Disorders 2RXi Pharmaceuticals Receives US Notice of Allowance for a Key Patent Relating to its Self-Delivering Technology with sd-rxRNAs targeting CTGF, including RXI-109, for the Treatment of Fibrotic Disorders 3Study: Fatigued Medical Interns Infect Their Patients with Herpes Viruses; The CBCD Sees a Parallel with “The Doctor’s Plague” 2Study: Fatigued Medical Interns Infect Their Patients with Herpes Viruses; The CBCD Sees a Parallel with “The Doctor’s Plague” 3
... of candidates worldwide - , , ... of Japan today announced the two fields eligible for the ... in science and technology. The 2010 Japan Prize will recognize ... production and environment. , , ...
... Nov. 28 Encorium Group, Inc.,(Nasdaq: ENCO ... studies in over 30 countries for many of the ... November,21, 2008, it received a letter from Nasdaq stating ... the quarterly period ended September 30, 2008 as of ...
... Information Services Inc., leads a videocast discussion with top executives ... manufacturing to Asia. , ... (PRWEB) ... in Atlanta, Georgia, Jim Miller, president of PharmSource Information Services ...
Cached Biology Technology:2010 Japan Prize to Honor Scientists and Researchers in Industrial Production/Production Technology and Biological Production/Environment Fields 22010 Japan Prize to Honor Scientists and Researchers in Industrial Production/Production Technology and Biological Production/Environment Fields 3Encorium Announces Receipt of Letter from Nasdaq Regarding its Third Quarter 2008 Form 10-Q Filing 2Encorium Announces Receipt of Letter from Nasdaq Regarding its Third Quarter 2008 Form 10-Q Filing 3PharmSource President Moderates Videocast on Outsourcing to Asia at AAPS 2
(Date:7/9/2014)... shark species may be able to cope with the rising ... , The Arctic today is best known for its tundra ... Roughly 53 to 38 million years ago during what is ... to a huge temperate forest with brackish water, home to ... creatures, crocodiles and giant tortoises. Much of what is known ...
(Date:7/9/2014)... infections usually announce themselves with pain and fever but often ... those that are sneaky and hard to beat. Now, scientists ... form of tiny DNA pyramids. Published in the journal ... nanopyramids can flag bacteria and kill more of them than ... that some infectious pathogens can lie in wait, undetectable in ...
(Date:7/9/2014)... of a sparrow-sized fossil from China challenges the commonly ... that gained the ability to fly. The birdlike fossil ... much rather the remains of a tiny tree-climbing animal ... the Dinosaur Museum in Blanding, Utah, and Alan Feduccia ... in Springer,s Journal of Ornithology . , The ...
Breaking Biology News(10 mins):Shark teeth analysis provides detailed new look at Arctic climate change 2Shark teeth analysis provides detailed new look at Arctic climate change 3Researchers declassify dinosaurs as being the great-great-grandparents of birds 2
... Cambridge, Mass. April 28, 2011 A project to ... a $100,000 grant from The Bill & Melinda Gates Foundation ... affiliate of the Harvard School of Engineering and Applied Sciences ... the aim is to develop a Microbial Fuel Cell-based charger ...
... new study shows for the first time that monkeys can ... ability is critical to our understanding of the evolution of ... to better diagnosing and treating memory impairments in humans. ... Primate Research Center researchers Ben Basile and Robert Hampton was ...
... is pleased to announce BIOLOGY OPEN (BiO), a new ... all aspects of the biological sciences including cell science, ... to provide rapid publication for peer reviewed, scientifically sound ... BIOLOGY OPEN will address some big issues in publishing ...
Cached Biology News:Harvard's engineering school receives $100,000 Grand Challenges Explorations grant 2Harvard's engineering school receives $100,000 Grand Challenges Explorations grant 3Monkey recall memory mirrors that of humans 2Announcing BIOLOGY OPEN from the Company of Biologists 2
MOUSE ANTI BOVINE TROPONIN I...
NORMAL DONOR DONKEY SERUM...
This BioSolution package is excellent for doing fluorescence thermal melt experiments on nucleic acids, studies involving biomolecular interactions, protein folding experiments, and more!...
acridine orange 10-nonyl bromide (nonyl acridine orange)...
Biology Products: