Navigation Links
New Fusion Trans-Activator Plasmids for Studying Signal Transduction,,,Pathways

Study the signal transduction pathway of your choice

Li Xu Chao-Feng Zheng
Stratagene Cloning Systems, Inc.

The PathDetect in vivo signal transduction pathway reporting systems are designed for assessing the in vivo activation of signal transduction pathways. The PathDetect trans-reporting systems include a unique fusion trans-activator plasmid that expresses pathway-specific trans-activator proteins fused to the yeast GAL4 DNA binding domain. To facilitate the construction and expression of new fusion trans-activator proteins, Stratagene has designed the new pFA-CMV plasmid for expressing the activation domain of any transcription factor fused to the yeast GAL4 DNA binding domain. The pFA-CMV plasmid features a multiple cloning site and the cytomegalovirus (CMV) promoter for convenient construction and high-level expression of fusion trans-activator proteins in a variety of mammalian cell lines. We describe two new fusion trans-activator plasmids (constructed with the pFA-CMV plasmid) expressing GAL4 fusions with c-Fos (208-313) and ATF2 (1-96).

figure 1

Recently, Stratagene introduced the PathDetect in vivo signal transduction pathway trans-reporting systems, which are designed for specific, rapid and convenient assessment of the in vivo activation of signal transduction pathways.1 These reporting systems are used for studying the in vivo effects of new genes, growth factors, drug candidates and extracellular stimuli on the activation of c-Jun N-terminal kinase (JNK),2,3 mitogen-activated protein kinase (MAPK),4,5 cyclic AMP-dependent protein (PKA)6 and other signaling molecules leading to the activation of these kinases. The PathDetect report
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
2. Stable HeLa Luciferase Reporter Cell Lines Expressing GAL4 Fusion Transactivators
3. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
4. Comparison of Performance between the AlphaQuest - HTS and the Fusion α Multilabel Reader for Detection of cAMP
5. Mobile Phase Optimization for the Analysis of an Antibody-Based Fusion Protein and Aggregates using a TSKgel Super SW3000 Size-Exclusion Column
6. New Reporter Plasmids for Studying Interferon-Stimulated Signal Transduction Pathways
7. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
8. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
9. Enzyme Immunoassay for Studying Intracellular Levels of cAMP
10. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
11. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements

Post Your Comments:
(Date:11/20/2009)... METTLER TOLEDO recently announced th...nar, “The Role of Process Analytical Technol...” on December 1, 2009. , ... Role of Process Analytical Technology (PAT) in Gr...cember 1, Process Analytical Technology (PAT) case...
(Date:11/19/2009)...LAUVELT, N.Y., Nov. 19 Dr. Mate Hi...ract, responds to a press release issued on Novemb...n company for making false and self-serving statem...s. ,, Dr. Hidvegi stated from his office in Bu... research on Avemar does not apply to AveULTRA, ...
(Date:11/19/2009)..., MALVERN, Pa. and WALTHAM, Mass., Nov. 19 /PRNew...cision Resources find that the leading driver of a...ope is broad-spectrum activity against gram-positi... of organisms that can cause nosocomial pneumonia,...k primarily for agents that provide appropriate co...
(Date:11/19/2009)... World,s Top Gene Synthesis Companies...C) to Coordinate Best Practices in Risk Reduction ...orldwide Gene Synthesis Capacity , ... world’s leading gene synthesis companies to...mon screening protocol to promote biosecurity in t...
Breaking Biology Technology:Process Analytical Technology (PAT) in Green Chemistry and Green Engineering 2American BioSciences Rebuts Misleading Statements Made by Hungarian Competitor 2Broad-Spectrum Antibacterial Activity is the Most Important Driver of Antibiotic Selection for Nosocomial Pneumonia In Europe 2Broad-Spectrum Antibacterial Activity is the Most Important Driver of Antibiotic Selection for Nosocomial Pneumonia In Europe 3World's Top Gene Synthesis Companies Establish Tough Biosecurity Screening Protocol 2World's Top Gene Synthesis Companies Establish Tough Biosecurity Screening Protocol 3World's Top Gene Synthesis Companies Establish Tough Biosecurity Screening Protocol 4
...chitectural rendering shows an aerial view of the ...sy of Hellmuth, Obata and Kassabaum Inc. and the Z... Rennebohm Foundation has contributed $15 million ...acilities on the University of Wisconsin-Madison,s...ild. , ,Called the Interdisciplinary Research Comp...
...in has landed two IT movers and shakers to Governm...eamers and Drivers. , ,State CIO Matt Miszewski ... Brookfield, recently were named to the list for t...he state,s IT infrastructure. Both men were featur... at Madison,s Fluno Center last week. , ,Kanavas s...
...hompson, former U.S. Health and Human Services sec...d head-first into several high-profile executive p.... , ,Thompson accepted three positions: senior adv...e USA; law partner at Washington power firm Akin B...se-based Logistics Health Inc., a privately held p...
Other Biology Technology:State IT leaders make Government Technology's top-25 list 2Tommy Thompson tackles the private sector in spades 2
(Date:11/23/2009)...ies reveal in unprecedented detail how the ribosom...roteins and guide them toward their destination in...mics flexible fitting (MDFF) to examine the intera... partners. , The first study, in Proceedings o...intimate signaling between the ribosome and an elo...
(Date:11/23/2009)...ds have existed for 360 million years and survived...us is threatening to make many of them extinct, ac...crobiology Today . , The fungus, Batrachochytr...d with waves of amphibian extinctions in Central A...Bd infects over 350 amphibian species by penetrati...
(Date:11/23/2009)...the gender of six-week-old turkey poults could sav...y after birth, according to Dr. Gerald Steiner fro...and his team. Their use of infrared spectroscopy t...t is a fast and accurate method with the potential...nd select female eggs for breeding. The pilot stud...
Breaking Biology News(10 mins):Computational microscope peers into the working ribosome 2Computational microscope peers into the working ribosome 3Rescuing male turkey chicks 2Sirnaomics Receives SBIR Grant from NIH NCI for Its Multi Targeted Lung Cancer siRNA Therapeutics 13154 1Sirnaomics Receives SBIR Grant from NIH NCI for Its Multi Targeted Lung Cancer siRNA Therapeutics 13154 2Sirnaomics Receives SBIR Grant from NIH NCI for Its Multi Targeted Lung Cancer siRNA Therapeutics 13154 3Carotid Artery Sections React Differently to Plaque 52445 1Carotid Artery Sections React Differently to Plaque 52445 2Many Young Adults Uninformed About Vaccines 52443 1Many Young Adults Uninformed About Vaccines 52443 2Many Young Adults Uninformed About Vaccines 52443 3
... MANHATTAN -- Kansas is lucky that a devastating w...t. Researchers at Kansas State University are gett...ture to keep it that way. , Barbara Valent, unive...is leading a group of researchers to study the whe...ous threat in Brazil. , "We don,t know if our var...
... MADISON In the rogues, gallery of microscopic in...in town., Warped pathogens that lack both DNA and ... ailments as chronic wasting disease (CWD) in deer...sheep and Creutzfeldt-Jakob disease in humans. In ...agent know to science, the prion is also the most ...
... PHILADELPHIA Genetic variations in DNA repair pa...s much as threefold or decrease it by as much as 7...ing to a report published in the January 15, 2009,... the American Association for Cancer Research. , ...ges, and thus is resistant to most available thera...
Other Biology News:Researchers to use K-State's BSL-3 Lab for $1 million study of fungus threatening wheat crops 2Researchers to use K-State's BSL-3 Lab for $1 million study of fungus threatening wheat crops 3Common soil mineral degrades the nearly indestructible prion 2
Rabbit Anti-Human Regulator of g-protein signaling 2 Polyclonal Antibody, Unconjugated from Lifespan Biosciences
Mouse Anti-TCR V beta 5 Monoclonal Antibody, Unconjugated, Clone MH3-2 from BD Biosciences Pharmingen
LightCycler ® 2.0 Instrument; PCR amplification system and detection system from Roche Applied Science
...runs both gel dimensions in the same cell. Up to 1...n a single run using the tube gel adaptors, which ... as the slab gel assembly. While the tube gels are...st in the casting stand. The 20 cm long slab gel o...
Biology Products: