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New Competent Cells for Highest Transformation Efficiencies

mix was used to transform both XL10-Gold cells and DH5a** cells (table 1). XL10-Gold ultracompetent cells yielded 27-fold more kanamycin-resistant transformants than DH5a cells. The pUC18 vector was used as the supercoiled control plasmid for this experiment, transforming XL10-Gold cells with a 5-fold higher transformation efficiency than DH5a cells.** These data support our finding that XL10-Gold cells have a significantly improved ability to transform larger (ligated or LIC-annealed) DNA molecules.

Transformation of cDNA Libraries

pUC18

pCMV-Script Library

pAD-GAL4 Library

Host


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