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New Competent Cells for Highest Transformation Efficiencies

for both XL10-Gold and XL2-Blue cells and 5-fold better than for DH10B cells.

Therefore, XL10-Gold ultracompetent cells retain their high efficiency when transforming pUC18 vector DNA and are significantly better at transforming the large supercoiled plasmid than either XL2-Blue or DH10B cells.

Transformation of Ligated DNA

DH5a cells

XL10-Gold cells

Colony Number (average of 3 experiments)

261

7250

pUC18 Efficiency (cfu/mg)

1 x 109

5 x 109

A second assay was performed to determine the relative efficiency of transforming XL10-Gold cells with a ligated DNA molecule. The pCAL-n-EK vector (8.0 kb) from Stratagenes Affinity LIC cloning kit was digested and prepared for the ligation-independent cloning (LIC) reaction using standard conditions.2 The kanamycin-resistance gene was annealed to the pCAL-n-EK vector. An aliquot of the annealed
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