( 5 x 10 2 ...)New Competent Cells for Highest Transformation Efficiencies
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>5 x 102
1
1 x 109
1
XL2-Blue cells
4 x 103
8
5 x 109
5
XL10-Gold cells
4 x 104
80
5 x 109
5
A 25-kb supercoiled plasmid, pRK2013, was transformed into three different
competent cell lines (figure
1): XL10-Gold, XL2-Blue cells and DH10B cells.**
The pRK2013 plasmid was selected to simulate the large DNA molecules that
exist when the transformation substrate is a ligated molecule. These data
show that XL10-Gold cells were up to 80-fold more efficient than DH10B
and 10-fold more efficient than XL2-Blue cells at transforming this large
plasmid. The efficiency with which supercoiled pUC18 transformed was the
same
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Source:
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