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New BL21-CodonPlus Cells Correct Codon Bias in GC-Rich Genomes

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Stratagenes BL21-CodonPlus competent cells resolve the problem of codon bias for expressing heterologous genes in E. coli. While the BL21-CodonPlus-RIL strain is designed for the expression of AT-rich genes, the new BL21-CodonPlus-RP cells specifically assist gene expression from GC-rich organisms. In both strains, extra copies of tRNA genes rescue expression of genes that would otherwise be restricted by the presence of rare codons; however, these additional genes cause no deleterious effects to the host cells. Consequently, BL21-CodonPlus-RIL and BL21-CodonPlus-RP competent cells are useful strains for standard expression in E. coli, even in the absence of a detected codon bias problem.

REFERENCES

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  4. Deana, A., Ehrlich, R., and Reiss, C. (1998) Nuc. Acids Res. 26: 4778-4782.

  5. Carstens, C.-P. and Waesche, A. (1999) Strategies 12: 49-51.

  6. Hu, X., et al. (1996) Protein Expr. Purif. 7: 289-293.

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** Patent pending


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