Navigation Links
New BL21-CodonPlus Cells Correct Codon Bias in GC-Rich Genomes

This in turn, leads to slowing or abortion of the translation process and subsequent degradation of the mRNA.4 When the problem of codon bias causes frameshifts, codon skipping, or misincorporations, it may escape detection. More commonly, manifestations of codon bias include no or low protein expression and the presence of aborted translation products. Previously, the typical remedies for overcoming codon bias were to alter the codon specifications of a target gene by site-directed mutagenesis or to circumvent the problem by switching to a eukaryotic expression system.

To make it easier to resolve the codon bias problem, Stratagene released BL21-CodonPlus-RIL competent cells,5 which are derivatives of the BL21-Gold series. BL21-CodonPlus-RIL cells contain a ColE1-compatible vector with extra copies of the argU, ileY, and leuW tRNA genes. These tRNAs recognize arginine, isoleucine, and leucine codons (AGA/AGG, AUA, and CUA), respectively. Host cell expression of these tRNAs can lead to increased synthesis of recombinant proteins, as translation is no longer limited by the availability of tRNAs that recognize rare codons. However, using these codons is predominantly a problem in organisms with AT-rich genomes (Table 1). For organisms whose genomes are GC rich, the problematic codons for expression are the arginine codon AGG (recognized by the tRNA product of the argU gene) and the proline codon CCC (recognized by the tRNA product of the proL gene).

Hence, Stratagene designed BL21-CodonPlus-RP and BL21-CodonPlus(DE3)-RP competent cells to solve this dilemma. These cells include a ColE1-compatible expression plasmid that contains the tRNA genes argU and proL. The corresponding tRNAs recognize the arginine codons, AGA/AGG, and the proline codon, CCC, respectively.

Rare Codons and Protein Production

Fig.1

We per
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
2. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
3. Cell Proliferation Kit Outperforms the Competition in the Range of Cells Typically Counted
4. XL10-Gold Cells Supplied in New Single-Reaction Format
5. Isolate and Analyze Total RNA from Cells Harvested by Laser Capture Microdissection
6. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
7. Simple Isolation of RNA from Tissue and Cultured Cells
8. TKB1 Cells Identify Receptor Tyrosine Kinase Interacting Proteins
9. Tools for Detecting MSH2 Expression in Chinese Hamster Ovary Cells
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: New CodonPlus Cells Correct Codon Bias Rich Genomes

(Date:12/24/2014)... , Dec. 23, 2014 Vermillion, Inc. ... on gynecologic disease, announced today that investors including ... Birchview Fund LLC and several Vermillion directors have ... shares of Vermillion,s common stock and warrants to ... a private placement.  Under the ...
(Date:12/22/2014)... 2014 The Center for Professional ... technical training across the life sciences industry, has partnered ... (SCDM) to provide the organization's members with discounted classroom ... to the more than 350 sessions across 80 course ... off when registering for a public course ...
(Date:12/22/2014)... Dec. 22, 2014  ( www.competitivehealth.com ) — Competitive ... medical bill review and advocacy service, has signed an ... WellCard Savings discount health services marketplace. 63% ... more than they expected to pay. As part of ... costs, WellCard Savings is pleased to offer medical bill ...
(Date:12/19/2014)... Lawrence, MA (PRWEB) December 19, 2014 ... flow MRLAFMQ test for the Detection of Aflatoxin M1 ... test to receive independent third party validation. The peer ... Science Unit of the Institute for Agricultural and Fisheries ... Francis Group. , Aflatoxin B1, the most toxic ...
Breaking Biology Technology:Vermillion Announces Equity Financing of up to $18.9 Million; Suspends ATM Program 2Vermillion Announces Equity Financing of up to $18.9 Million; Suspends ATM Program 3Vermillion Announces Equity Financing of up to $18.9 Million; Suspends ATM Program 4Vermillion Announces Equity Financing of up to $18.9 Million; Suspends ATM Program 5CfPIE Announces Partnership with the Society for Clinical Data Management 2CfPIE Announces Partnership with the Society for Clinical Data Management 3WellCard Savings Announces CoPatient Medical Bill Review Service for Members 2Charm Sciences Achieves Independent Validation of First 15 Minute Quantitative Screening Method for Aflatoxin M1 2
... 17 Sigma-Aldrich (Nasdaq:,SIAL) today announced the publication ... accessible Journal of Visualized Experiments (JoVE).,This makes Sigma-Aldrich ... video,protocol to the traditional scientific publication process. Peer-reviewed,video ... to increase the,efficiency of scientific communication and with ...
... CryoLife, Inc. (NYSE: CRY ),a biomaterials, medical device ... to participate in the upcoming UBS Global Life,Sciences Conference ... City., D. Ashley Lee, executive vice president, chief ... present Wednesday,September 24 at 2:30 p.m., Eastern Time., ...
... BAX ), will host a quarterly conference call ... October 16, 2008, at,8:30 a.m. Eastern Time (7:30 a.m. ... (domestic) or 703-639-1156 (international). Please dial,into the call at ... call for the,operator to connect you., This call ...
Cached Biology Technology:Sigma-Aldrich(R) Becomes First Life Science Company to Publish in PubMed's Peer-Reviewed Video Journal JoVE 2Sigma-Aldrich(R) Becomes First Life Science Company to Publish in PubMed's Peer-Reviewed Video Journal JoVE 3CryoLife to Present at UBS Global Life Sciences Conference 2Baxter International Third Quarter 2008 Financial Results Conference Call 2
(Date:12/11/2014)... 09, 2014 Research and Markets ... ( http://www.researchandmarkets.com/research/twhjll/biometrics_market ) has announced the addition ... Region 2015-2019"  report to their offering. ... is advances in technology. With continuous advances in ... to the latest standard that meets the needs ...
(Date:12/10/2014)... YORK , Dec. 8, 2014 You,ve been ... banking account but can,t remember your password, site key or ... Who was your first grade teacher? Today, ... the app that will finally put an end ... – 1U TM . 1U leverages a user,s ...
(Date:12/10/2014)... Dec. 08, 2014 Research and Markets ... of the "Biometrics Market in Japan 2014-2018" ... http://photos.prnewswire.com/prnh/20130307/600769 The integration ... as rural banking and upgradation of the driver,s ... in the market. Besides the aforementioned projects, biometrics ...
Breaking Biology News(10 mins):Biometrics Market in the APAC Region 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 3Biometrics Market in Japan 2014-2018: Key Vendors are DDS, Fujitsu, Hitachi and NEC 2
... 2013 EcoHealth Alliance, a nonprofit organization that focuses on ... a new SARS-like coronavirus (CoV) in Chinese horseshoe bats. ... an international group of collaborators have uncovered genome sequences of ... erupted in Asia in 2002 2003, which caused a ...
... It,s not unusual for scientists to take their cues from ... career on such cues. Rimer, an assistant professor of ... College of Engineering, is an expert in the field of ... from drug development to chemical synthesis to medical diseases such ...
... New York City Mayor Michael Bloomberg prepares to leave office, ... health policies and concludes that he is an urban innovator ... infectious diseases to upstream risk factors in everyday life and ... Hastings Center Report . , Lawrence O. Gostin, University ...
Cached Biology News:New SARS-like coronavirus discovered in Chinese horseshoe bats 2New SARS-like coronavirus discovered in Chinese horseshoe bats 3New SARS-like coronavirus discovered in Chinese horseshoe bats 4Taking a cue from nature 2Bloomberg's health legacy: Urban innovator or meddling nanny? 2Bloomberg's health legacy: Urban innovator or meddling nanny? 3
... Rack inserts fit into the ... allow you to greatly increase ... deepwell format plates at an ... from the shaker at once ...
For quantitative determination of Aldolase in Serum (an indicator of muscle damage). Ready-to-use liquid stable reagent. Formulated to eliminate interference from pyruvates. Application sheets are ...
Request Info...
... ,5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside C 14 H 15 ... ,M.W.= 408.63 ,Assay: Greater than ... DMF) ,Biological Activity: Blue color with ... Agrees with theoretical values ,Thin Layer ...
Biology Products: