Navigation Links
Neurite outgrowth cell-by-cell analysis using the IN Cell Developer Toolbox

>


Results
Images from retinoic acid-induced neurite outgrowth were acquired with the IN Cell Analyzer 3000 and analyzed by both the IN Cell Analyzer 3000 Neurite Outgrowth Image Analysis Module and the IN Cell Developer Toolbox.

The IN Cell Analyzer 3000 software analyzes neurite outgrowth on the basis of population (i.e., the parameters are quantitated for the whole population and then reported either as a population total or averaged per nuclei or neurite correspondingly). The IN Cell Developer Toolbox allows users to analyze neurite outgrowth on a cell-by-cell basis. This permits a wider range of parameters, both population and individual cell-based, to be reported.

We have performed Pearson’s correlation analysis to compare results obtained using IN Cell Developer Toolbox and IN Cell Analyzer 3000 software. The retinoic acid dose-response neurite outgrowth experiment included 12 replicate wells for each of the six retinoic acid concentrations. Analysis with IN Cell Developer Toolbox was performed based on the described protocol and average neurite length results obtained using IN Cell Developer Toolbox and IN Cell Analyzer 3000 are shown (Fig 13). The Pearson’s correlation test demonstrates significant correlation (P < 0.001) between data, the data obtained using two analysis methods.


Conclusion
Neurite outgrowth can be quantitated on a cell-by-cell basis using IN Cell Developer Toolbox. The software allows the user to design a flexible protocol that can be adjusted to meet any specific assay requirements. A variety of approaches can be used for definition of cell regions within neurite networks. A wide choice of parameters is available for characterization of neurite outgrowth in analyzed samples. Population related results obtained using the IN Cell Dev
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. High-throughput gene expression analysis to screen for anti-asthma drugs
2. Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci
3. Flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer and on-chip staining
4. 2100 expert software Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer
5. Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis
6. Microfluidic analysis of multiplex PCR products for the genotyping of Helicobacter pylori
7. High resolution DNA analysis with the DNA 500 and DNA 1000 LabChip kits
8. Extended data analysis using DeCyder EDA
9. 2D-LC analysis of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
10. Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
11. TSKgel ODS-100Z and TSKgel ODS-100V column series provides a universal solution for the analysis of samples containing acidic, basic, polar & non-polar compounds
Post Your Comments:
(Date:1/15/2014)... (PRWEB) January 15, 2014 DTS Language ... its Online Web Portal for Life Science organizations who need ... specify the subject matter of their documents in advance with ... help reduce time-to-delivery of translations, often a critical factor in ...
(Date:1/15/2014)... 2014 AudioNotch is the internet's leading ... the treatment of tinnitus. Patients listen to sound therapy ... a period of weeks to months, their tinnitus volume decreases. ... forms: Notched Music and Notched White Noise. Now, AudioNotch is ...
(Date:1/14/2014)... Washington, DC (PRWEB) January 14, 2014 ... session for Thursday, January 23, 2014 at 2pm EST ... Actionable Knowledge.” The topic focuses on how technology can ... mission critical decisions for government agencies. The online webinar ...
(Date:1/14/2014)... As pet owners drew up their lists of New Year,s resolutions ... care of my furry companion." Nowadays there are pet salons, dog ... to take the little canine or feline darlings along wherever mommy ... some pricey toys at the pet store. But anyone who has ...
Breaking Biology Technology:DTS Improves Efficiency for Life Science Document Translations 2Webcast - Natural Language Processing: Converting Raw Data into Actionable Knowledge – Hosted by Carahsoft and CDS Federal Services 2New Year's Resolution: Give Pets the Gift of Top-Notch Health Care 2
... 15 NeoStem, Inc. (NYSE Amex: NBS ), which ... stem cells for future medical need, was featured on CBS2 News, ... and Tuesday morning July 14, 2009. , , ... of Dr. Christopher Centeno, a NeoStem consultant in stem cell therapies ...
... EXTON, Pa., July 15 Morphotek((R)), Inc., a subsidiary ... (CII), a privately held biotechnology company specializing in the development ... an evaluation and option agreement in which Morphotek will evaluate ... , The agreement with CII provides ...
... ... organizations was a key factor in the recent decision by the University of Florida Shands ... informatics needs. With the signing of the contract, the UFSCC has become the 25th ... ...
Cached Biology Technology:NeoStem Featured on CBS2 News; Success of Licensed Stem Cell Treatments Shown for Arthritis and Orthopedic Problems; Procedure to be Used by NeoStem Affiliate in China 2NeoStem Featured on CBS2 News; Success of Licensed Stem Cell Treatments Shown for Arthritis and Orthopedic Problems; Procedure to be Used by NeoStem Affiliate in China 3NeoStem Featured on CBS2 News; Success of Licensed Stem Cell Treatments Shown for Arthritis and Orthopedic Problems; Procedure to be Used by NeoStem Affiliate in China 4Morphotek(R), Inc. and Cancer Innovations, Inc. Announce Evaluation and Option Agreement for Therapeutic Monoclonal Antibodies 2Morphotek(R), Inc. and Cancer Innovations, Inc. Announce Evaluation and Option Agreement for Therapeutic Monoclonal Antibodies 3University of Florida Shands Cancer Center Selects OnCore(r) to Support its Collaborative Research Goals 2University of Florida Shands Cancer Center Selects OnCore(r) to Support its Collaborative Research Goals 3University of Florida Shands Cancer Center Selects OnCore(r) to Support its Collaborative Research Goals 4University of Florida Shands Cancer Center Selects OnCore(r) to Support its Collaborative Research Goals 5
(Date:7/10/2014)... cutting-edge, innovative research on Alzheimer,s disease and the ... announced the recipients of new research grant awards, ... the District of Columbia, and five foreign countries. ... latest grants, BrightFocus has now provided more than ... The research projects funded this year reflect a ...
(Date:7/10/2014)... strong currents in the Straits of Mackinac reverse direction ... beneath the channel would quickly contaminate shorelines miles away ... new University of Michigan study commissioned by the National ... study and accompanying animations, oil from a hypothetical pipeline ... hours and Bois Blanc Island after two days. All ...
(Date:7/10/2014)... (July 10, 2014) Columbia University Medical Center (CUMC) ... therapies for patients with retinitis pigmentosa (RP), a leading ... its kind, takes advantage of induced pluripotent stem (iPS) ... which are then used as a patient-specific model for ... researchers led by Stephen H. Tsang, MD, PhD, showed ...
Breaking Biology News(10 mins):BrightFocus Foundation announces 55 new grantees in Alzheimer's and vision research 2BrightFocus Foundation announces 55 new grantees in Alzheimer's and vision research 3Straits of Mackinac 'worst possible place' for a Great Lakes oil spill 2Straits of Mackinac 'worst possible place' for a Great Lakes oil spill 3Patient-specific stem cells and personalized gene therapy 2Patient-specific stem cells and personalized gene therapy 3
... simple technique to make a common virus-killing material significantly more ... Andrew Barron and Qilin Li. Rather than trying to ... into the public domain. They hope wide adoption will save ... professors and their team reported in Environmental Science and ...
... ITHACA, N.Y. Scientists have identified the genes related ... for planting crops closer together, which has led to an ... Genetics , Jan. 9, 2011.) The study, led ... Agricultural Research Service (USDA-ARS) at Cornell and North Carolina ...
... MD (January 11, 2011)-- The Genetics Society of America ... Research Conference, March 30-April 3, 2011 at the Town ... This annual meeting brings together basic research scientists, ... the common fruit fly, whose hereditary traits Thomas Hunt ...
Cached Biology News:Virus killer gets supercharged 2Virus killer gets supercharged 3Technique allows researchers to identify key maize genes for increased yield 252nd Genetics Society of America Drosophila Research Conference announced 2
BD Falcon, microtest ELISA plate 96 well flat-bottom, clear. Polystyrene, nonsterile, no lid (25/sp, 100/ca) Packaging: 25 / pack, 100 / case ...
... The Chemicon Detergent-OUT Kit is a ... from protein solutions. Removes detergents without ... load the protein solution on the Detergent-OUT ... solution. Micro columns are suitable for removing ...
... is a simple high performance method for ... detergents without significant dilution of protein solution. ... the Detergent-OUT Spin column and collect detergent ... suitable for removing detergent from a maximum ...
... microarrays are derived from frozen embedded tissue ... control procedures to ensure the highest quality. ... in one block, sectioned at a thickness ... slides. Sections are large (3-5 mm in ...
Biology Products: