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Neurite outgrowth cell-by-cell analysis using the IN Cell Developer Toolbox

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Results
Images from retinoic acid-induced neurite outgrowth were acquired with the IN Cell Analyzer 3000 and analyzed by both the IN Cell Analyzer 3000 Neurite Outgrowth Image Analysis Module and the IN Cell Developer Toolbox.

The IN Cell Analyzer 3000 software analyzes neurite outgrowth on the basis of population (i.e., the parameters are quantitated for the whole population and then reported either as a population total or averaged per nuclei or neurite correspondingly). The IN Cell Developer Toolbox allows users to analyze neurite outgrowth on a cell-by-cell basis. This permits a wider range of parameters, both population and individual cell-based, to be reported.

We have performed Pearson’s correlation analysis to compare results obtained using IN Cell Developer Toolbox and IN Cell Analyzer 3000 software. The retinoic acid dose-response neurite outgrowth experiment included 12 replicate wells for each of the six retinoic acid concentrations. Analysis with IN Cell Developer Toolbox was performed based on the described protocol and average neurite length results obtained using IN Cell Developer Toolbox and IN Cell Analyzer 3000 are shown (Fig 13). The Pearson’s correlation test demonstrates significant correlation (P < 0.001) between data, the data obtained using two analysis methods.


Conclusion
Neurite outgrowth can be quantitated on a cell-by-cell basis using IN Cell Developer Toolbox. The software allows the user to design a flexible protocol that can be adjusted to meet any specific assay requirements. A variety of approaches can be used for definition of cell regions within neurite networks. A wide choice of parameters is available for characterization of neurite outgrowth in analyzed samples. Population related results obtained using the IN Cell Dev
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