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Neurite outgrowth cell-by-cell analysis using the IN Cell Developer Toolbox

d. Select Remove Single Pixel Targets and Filled options in the Target Details panel.

5. Create a linked One to Many target set (‘Cells with neurites’) to link ‘Cell body’ (primary target set) with ‘Neurites’ (secondary target set). Set Overlap conditions as required (e.g., any intersection secondary target within primary target). To count each neurite only once, do not choose the option Allow multiple primary targets to share secondary targets.

6. Generate User Defined Measurements to report, for e. Number of neurites per cell
example:
a. Number of cells with neurites: [Count<\cell with neurites\All:Neurites>]
b. Length of individual neurites [Fiber Length<\cell with Neurites\Neurites>]
c. Total length of neurites per cell [Fiber Length<\cell with Neurites\All:Neurites>]
d. Total length of neurites per population [Fiber Length<\cell with Neurites\ Neurites>] Select Statistical function - Sum for this measure.
e. Number of neurites per cell [Count<\cell with neurites\All:Neurites>]
f. Population average number of neurites per cell [Count<\cell with neurites\All:Neurites>] Select Statistical function - Mean for this measure.
g.
Population average neurite length [Fiber Length<\cell with Neurites\ Neurites>] Select Statistical function - Mean for this measure.

7. Export the summary table. Analyze the data.


Processing steps
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