Navigation Links
Neurite outgrowth cell-by-cell analysis using the IN Cell Developer Toolbox

B>option in the Target Details panel.
c. Select Preprocessing operations if required.
d. Select Segmentation option that will identify whole cells (cell bodies and neurites) (Fig 6).
e. Use Erode operation to exclude neurites from the bitmap (Fig 7).
f. Use the Sieve operation to filter out debris mainly remaining from the joint points.
g. Use the Dilate operation with the same kernel settings as the previous Erode step to restore the cell bodies bitmap so that the outer edge of the mask coincides with the cell bodies edge but no neurites are included (Fig 8).
h. Use a second Dilate step with minimal kernel size in order to overlap the cell bodies bitmap with the neurites bitmap.
i. Apply Clump Breaking operation using ‘Nuclei’ as ‘seed’ (Fig 9).
j. Choose Count measurement and apply Statistical Function - Count to report total number of cell bodies in the population.

4. Create target set: ‘Neurites’.
a. Select channel with the image of neurites (Fig 5).
b. Create a macro that includes the following operation: Subtraction of the dilated cell bodies bitmap (Step 3g) from the whole cells bitmap (Step 3d). Include this macro in the protocol as ‘Preprocessing Macro’.
c. Select Intensity Segmentation option to create a mask from the output of the preprocessing operation by setting the lower intensity threshold above 0 (Fig 10).

'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. High-throughput gene expression analysis to screen for anti-asthma drugs
2. Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci
3. Flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer and on-chip staining
4. 2100 expert software Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer
5. Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis
6. Microfluidic analysis of multiplex PCR products for the genotyping of Helicobacter pylori
7. High resolution DNA analysis with the DNA 500 and DNA 1000 LabChip kits
8. Extended data analysis using DeCyder EDA
9. 2D-LC analysis of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
10. Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
11. TSKgel ODS-100Z and TSKgel ODS-100V column series provides a universal solution for the analysis of samples containing acidic, basic, polar & non-polar compounds
Post Your Comments:
(Date:8/21/2014)... Calif. , Aug. 21, 2014  Four-dimensional ... and behaviors based on external stimuli such as ... to have a disruptive effect in multiple industries.  ... the business landscape by printing objects ranging from ... and automotive sectors. Logo - ...
(Date:8/21/2014)... 2014 Ontotext S4 , The ... Ontotext . Now the same enterprise hardened text ... and unstructured data is available to start-ups and mid-size ... technology. Organizations that do not have resources to evaluate ... use S4 since there is no need for on ...
(Date:8/21/2014)... 2014 SoundConnect , a ... as one of the nation’s Fastest Growing Private ... the 2nd consecutive year. Inc. magazine today ranked ... 500|5000, an exclusive ranking of the nation's fastest-growing ... look at the most important segment of the ...
(Date:8/20/2014)... RALEIGH, N.C. , Aug. 20, 2014 /PRNewswire/ ... clinical research services located in Raleigh, ... Bradford Evans as Vice President of ... Administration, Brad will oversee all corporate processes, including ... to joining Clintrax Global, Brad worked as an ...
Breaking Biology Technology:Frost & Sullivan: 4-D Printing to Usher in Age of Low-Labor, Fast-Paced Product Manufacturing 2Frost & Sullivan: 4-D Printing to Usher in Age of Low-Labor, Fast-Paced Product Manufacturing 3Frost & Sullivan: 4-D Printing to Usher in Age of Low-Labor, Fast-Paced Product Manufacturing 4Ontotext Releases Text Mining & Semantic Technology Running in the Cloud – Welcome to “S4? 2Ontotext Releases Text Mining & Semantic Technology Running in the Cloud – Welcome to “S4? 3Ontotext Releases Text Mining & Semantic Technology Running in the Cloud – Welcome to “S4? 4SoundConnect Returns on Inc. 5000 List of Fastest Growing Companies 2SoundConnect Returns on Inc. 5000 List of Fastest Growing Companies 3Clintrax Global, Inc. Announces Addition to Executive Team 2
... overwhelming to Governor Jim Doyles proposal to invest nearly ... biotechnology future. Budget-conscious members of the Legislature have choked ... once again protested, and Milwaukee politicians have asked if ... should address all legitimate gripes about the specifics of ...
... see some competition soon. A coalition of research ... plans for an incubator for start-up biotech companies. , ... to fit new companies in, said representatives of the ... room to attract companies to the area. , ,Guy ...
... paced clock in 2004 as the state became better known ... welcome. , ,Dont pop the Champagne corks just yet: Wisconsin ... is creating as many high-wage jobs as the state lost ... the creation of globally competitive companies, while closing, is still ...
Cached Biology Technology:Wisconsin cannot afford to take a pass on Doyles biotech plan 2Wisconsin cannot afford to take a pass on Doyles biotech plan 3Milwaukee research alliance sees need for incubator 2Ten resolutions that could produce a happy new (tech) year 2Ten resolutions that could produce a happy new (tech) year 3
(Date:8/21/2014)... It consists of radiations in the 3.3 to 4.4 ... in biological tissues. New theoretical findings show that it ... window. These could be the basis of an optimal ... samples or to be used in high-precision spectroscopy. Now, ... to efficiently generate the harmonic radiationswhich are multiples of ...
(Date:8/21/2014)... published in the journal Carcinogenesis by researchers ... for the protein adenomatous polyposis coli (APC) in suppressing ... in the U.S. , Lead author Kristi Neufeld, associate ... of the Cancer Biology program at the KU Cancer ... trying to understand the various activities of APC, a ...
(Date:8/21/2014)... researchers at Louisiana Tech University has developed ... 3D printers and materials to fabricate custom ... chemotherapeutic compounds for targeted drug delivery. , ... research faculty from Louisiana Tech,s biomedical engineering ... filament extruders that can make medical-quality 3D ...
Breaking Biology News(10 mins):Water window imaging opportunity 2Research offers insight into cellular biology of colorectal cancer 2Research offers insight into cellular biology of colorectal cancer 3Louisiana Tech University researchers use 3D printers to create custom medical implants 2Louisiana Tech University researchers use 3D printers to create custom medical implants 3
... use of androgen deprivation therapies to prevent precancerous prostate ... effects in men with precancers with specific genetic alterations, ... in Cancer Discovery , a journal of the ... and survival of prostate cancer cells are very dependent ...
... find the easiest and fastest way to test for harmful ... article published in Frontiers in Microbiology on the 20th of ... (PCR) system to discover specific gene targets that indicate the ... performed using this PCR system are rapid, sensitive, and reliable. ...
... Humpback whales might be expected to take their food seriously ... they may multi-task as they eat, singing mating or breeding ... research, published December 19 in the open-access journal PLOS ... and colleagues, sheds new light on the whales, singing habits ...
Cached Biology News:Preventing prostate cancer through androgen deprivation may have harmful effects 2USDA explores using novel genetic labs for faster detection of E. coli 2Music with dinner: Whales sing during foraging season, not just while breeding 2Music with dinner: Whales sing during foraging season, not just while breeding 3Music with dinner: Whales sing during foraging season, not just while breeding 4Music with dinner: Whales sing during foraging season, not just while breeding 5
... (monoclonal) Nop1p/Fibrillarin was originally ... bakers yeast, Saccharomyces cerevisiae (accession P15646). ... yeast viability and is localized in ... Nop1p is fibrillarin (accession P22087) a ...
... many of the automation concepts from High ... are similar to those for HTS: ... or 48 wells with well volumes of ... number of wells has increased to 96 ...
... The Reverse-iT™ One-Step Kit is the ... in RT-PCR. It has been specifically ... The Reverse-iT™ One-Step Kit combines ... and ABgene®s Thermoprime Plus DNA Polymerase ...
... Synthetic Peptide: M(1) E F L P E ... T M D L E(19) PA1-072 detects munc-18 ... been successfully used in Western blot procedures. ... kDa protein representing Mint3 from AtT20 cell extract.,PA1-072 ...
Biology Products: