Navigation Links
Neurite outgrowth cell-by-cell analysis using the IN Cell Developer Toolbox

th Image Analysis Module


Other materials required
Mouse renoblastoma neuro-2a (N2a) cells (ATCC)

Retinoic acid (Sigma)

Monoclonal anti-α-tubulin clone DM1A (Sigma)

Anti-mouse IgG-FITC antibody (Sigma)

Hoechst™33342 (Molecular Probes)


Assay procedure
Retinoic acid-induced neurite outgrowth was measured by quantitation of neurite extensions from fluorescently labeled neuronal cells. In the indirect immunoassay used here, FITC-conjugated secondary antibody detects anti-neurofilament 200 kD subunit (NF200) antibody. Cells were counter-stained with Hoechst 33342 to identify nuclei and imaged using the IN Cell Analyzer 3000.


Analysis procedure
1. Open image stack in the IN Cell Developer Toolbox.

2. Create target set: ‘Nuclei’
a. Select the channel with the image of the nuclei marker (Fig 2).
b. Select Preprocessing operations if required.
c. Select Segmentation option most appropriate for an accurate nuclear segmentation (Fig 3).
d. Use Erode and Sieve Postprocessing operations to refine nuclei bitmap (Fig 4).
e. Choose Count measurement and apply Statistical Function – Count to report total number of nuclei in the population.

3. Create target set: ‘Cell body’.
a. Select channel with the image of the neurite (Fig 5).
b. Select Remove Single Pixel Targets '"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. High-throughput gene expression analysis to screen for anti-asthma drugs
2. Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci
3. Flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer and on-chip staining
4. 2100 expert software Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer
5. Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis
6. Microfluidic analysis of multiplex PCR products for the genotyping of Helicobacter pylori
7. High resolution DNA analysis with the DNA 500 and DNA 1000 LabChip kits
8. Extended data analysis using DeCyder EDA
9. 2D-LC analysis of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
10. Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
11. TSKgel ODS-100Z and TSKgel ODS-100V column series provides a universal solution for the analysis of samples containing acidic, basic, polar & non-polar compounds
Post Your Comments:
(Date:6/3/2015)... 2015  HireLifeScience.com, the premier career networking website ... 6 th annual career fair at The ... in Edison, NJ on ... Covance, Actavis and AstraZeneca.  Additional Pharmaceutical, Biotechnology and ... Johnson & Johnson, MedImmune, DSM Nutritional Products, LifeCell, ...
(Date:6/3/2015)... SHANGHAI and CAMBRIDGE, Mass. and REYKJAVIK, ... NextCODE , a precision medicine company using the genome ... been selected by Genomics England (GeL) to ... both rare disease and cancer patients as part of ... of the UK National Health Service (NHS). ...
(Date:6/3/2015)... GEA's Panther™ NS3006L is ... standalone, fully-equipped laboratory unit that is designed for continuous ... pilot plants. The Panther™ NS3006L achieves the same results ... for easy installation with simple operation, quick maintenance and ... be used in many different industries, such as food, ...
(Date:6/2/2015)... - Shell today celebrated the North American launch of an ... exhibit the size of a city block in New ... and the public, and special appearances by industry leaders ... the best total engine protection by addressing three prominent ... corrosion. "With the launch of Shell V-Power ...
Breaking Biology Technology:AstraZeneca Added as Sponsor for Industry Leading Life Science Hiring Event 2WuXi NextCODE Wins Genomics England Clinical Interpretation Tender in both Rare Disease and Cancer 2GEA Announces the Panther™ NS3006L, a Self-Contained, Fully-Equipped Laboratory Homogenizer Well Suited for Pilot Plants 2New Shell V-Power NiTRO+ Premium Gasoline Introduces Breakthrough Formulation for BEST TOTAL Engine Protection 2New Shell V-Power NiTRO+ Premium Gasoline Introduces Breakthrough Formulation for BEST TOTAL Engine Protection 3New Shell V-Power NiTRO+ Premium Gasoline Introduces Breakthrough Formulation for BEST TOTAL Engine Protection 4
... (Nasdaq: SGMO ) announced today that Edward ... on Sangamo,s diabetic neuropathy,programs at 1:40 p.m. (ET) ... Adams,Diabetes and Obesity Conference in San Francisco., ... be accessed via a link on,the Sangamo BioSciences ...
... Mass., May 29 Haemonetics,Corporation and Octapharma ... multi-year,supply contract appointing Haemonetics as Octapharma,s preferred ... States. Under the agreement,Octapharma will use Haemonetics, ... its U.S. plasma collection centers. Plasma is ...
... BALTIMORE, May 29 FASgen Diagnostics, LLC ... they have entered into,a collaboration to produce ... biomarkers,using FASgen,s proprietary antibodies. The tests measure ... (FAS), which is found in,virtually all solid ...
Cached Biology Technology:Sangamo BioSciences to Present at the Canaccord Adams Diabetes and Obesity Conference 2Haemonetics and Octapharma AG Announce Plasma Collection Technology Contract 2FASgen Diagnostics, LLC Announces Production and Investment Agreement With Tetracore, Inc. 2
(Date:5/11/2015)...  Through a well-rounded UAS delegation representing private industry, government, ... showing at AUVSI,s Unmanned 2015 conference last week in ... UAS industry met with over 200 hundred people ... the UAS ecosystem. "Our message is clear ... Aerospace Rich Knoll . "If you want to fly ...
(Date:5/8/2015)... 2015 Synaptics Inc. (NASDAQ: SYNA ), the ... of the executive management team will present at the following ... and Telecom Conference Date: May 18, 2015 Time: ... MA Cowen and Company Technology, Media ... Location: The New York Palace Hotel, New York, ...
(Date:5/5/2015)... -- NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" or the ... commerce market, reminds investors and media that  Mr. David ... CARTES SECURE CONNEXIONS AMERICA 2015, held in Washington ... three-day conference is organized into a series of nine session ... Global Fraud: Where is the Trust in Cyberspace? and ...
Breaking Biology News(10 mins):Ohio Flies High at Unmanned '15, Sets Stage for Ohio UAS Conference 2Synaptics to Present at Upcoming Investor Conferences 2NXT-ID, Inc.'s CTO, David Tunnell, Presents at CARTES SECURE CONNEXIONS AMERICA 2015 Today in Washington DC. 2NXT-ID, Inc.'s CTO, David Tunnell, Presents at CARTES SECURE CONNEXIONS AMERICA 2015 Today in Washington DC. 3
... CLEVELANDTwo teams of researchers, including a scientist from Case ... new species of fossil horse from 4.4 million-year-old fossil-rich ... the size of a small zebra, Eurygnathohippus woldegabrieli ... at Case Western Reserve in 1987had three-toed hooves and ...
... is available in German . ... have been announced. On Thursday, 5 December 2013, the Joint Committee ... ,four women and seven men as winners of the ... the Nominations Committee from 129 nominees. Four of the eleven prizewinners ...
... study shows that two unusual treatment approaches may have beneficial ... with the disorder. Using a hot bath to raise body ... worm eggs to stimulate the production of immunoregulatory factors in ... autism. These findings support the idea that disruption of systems ...
Cached Biology News:A new species of horse, 4.4 million years old 2A new species of horse, 4.4 million years old 3Leibniz Prizes 2014: DFG honors 11 outstanding researchers 2Leibniz Prizes 2014: DFG honors 11 outstanding researchers 3Leibniz Prizes 2014: DFG honors 11 outstanding researchers 4Leibniz Prizes 2014: DFG honors 11 outstanding researchers 5Leibniz Prizes 2014: DFG honors 11 outstanding researchers 6Leibniz Prizes 2014: DFG honors 11 outstanding researchers 7Leibniz Prizes 2014: DFG honors 11 outstanding researchers 8Leibniz Prizes 2014: DFG honors 11 outstanding researchers 9Leibniz Prizes 2014: DFG honors 11 outstanding researchers 10Leibniz Prizes 2014: DFG honors 11 outstanding researchers 11Leibniz Prizes 2014: DFG honors 11 outstanding researchers 12Leibniz Prizes 2014: DFG honors 11 outstanding researchers 13Worms and hot baths: Novel approaches to treating autism 2
...
One-step, microplate or cuvet, turbidometric, linear detection range 0.096 mg/dL to 11.5 mg/dL. Procedure: 5 min....
One-step, microplate or cuvet, colorimetric, detection range 0.1 mg/dL to 3 mg/dL. Procedure: 10 min....
Moue Serum 8-12 Weeks Old...
Biology Products: