Navigation Links
Near Infrared Technology and Optical Agents For Molecular Imaging

1, 8.



In a cell based assay system using IRDye 800CW labeled secondary antibodies to assess cell signaling pathways, IRDye 800CW was shown to yield superior signal-to-background ratios and enabled quantification of low levels of protein phosphorylation9.

In contrast, the signal to background using Cy5.5- labeled secondary antibodies was too low to allow its use in the assay (data not shown). Further experiments showed that Cy5.5 exhibited a high level of non-specific binding to cells (data not shown). This observation, coupled to the fact that Cy5.5 is outside the optimal NIR region, makes it less suitable for imaging studies requiring high signal to background.

The performance of IRDye 800CW has been compared to radiochemical detection in animal studies. Using gamma scintigraphy and NIR imaging, Houston et al . compared the ability of a cyclopentapeptide dual-labeled with 111indium and I R D ye 800CW to image av3-integrin positive melanoma xenografts10. The tumor regions were clearly delineated by optical imaging of the IRDye 800CW signal. In contrast, the tumor boundaries could not be identified by scintigraphy due to high noise levels.

We have successfully used IRDye 800CW conjugated to epidermal growth factor (IRDye 800CW-EGF) as an optical agent for imaging tumor progression11. Figure 2 shows an example of a prostate tumor-bearing SCID mouse injected with IRDye 800CWlabeled EGF. The IRDye 800CW-EGF showed good sensitivity with very low background from autofluorescence or non-specific binding.



For animal imaging, targeted NIR-labeled ligands can be used to visualize virtually any pathology without the need for engineered cell lines as required for imaging by bioluminescence or f
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Innovative Tissue Array Technology for High-Throughput Screening of Gene Expression
2. Enhancing Ruggedness and Full-Scan MS Sensitivity Using Ion Sweep Technology
3. Novel MicroRNA Array Technology for Sensitive miRNA Profiling
4. Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor
5. Use of Novel FlashPlate Technology to Measure cAMP Accumulation in Chinese Hamster Ovary Cells Expressing Human -2 Adrenoreceptors
6. Use of FlashPlate Technology for In Vitro Measurement of 125I-Labeled TGF-1 Binding on Chimeric Extracellular Domain of Type II Transforming Growth Factor Receptor
7. DNA purification from pig ears to bird blood using proven DNeasy Technology
8. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
9. Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon Allelic Discrimination Kits
10. Genotyping Single Nucleotide Polymorphisms with Molecular Beacons
11. New Protocols for Isolating High- Molecular-Weight Genomic DNA
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Near Infrared Technology and Optical Agents For Molecular Imaging

(Date:7/11/2014)... -- Researchers have pioneered a revolutionary new way to ... Virtual Finger, allows scientists to move through digital ... using the flat surface of their computer screens. ... orders of magnitude more efficient, saving time, money ... areas of experimental biology. The software and its ...
(Date:7/10/2014)... UK (PRWEB) July 10, 2014 ... 21st Century Medicine Forum on ‘Commercialising Longevity Research’ ... and entrepreneurs to the London Bioscience Innovation Center ... Research Foundation and Aging Analytics Ltd. The meeting ... investment in translational research for age-related disease, as ...
(Date:7/10/2014)... Robert Harman, DVM, Founder and CEO of Vet-Stem, Inc., the ... relaunch of his highly informative blog, now named Stem Cells ... are Stem Cells ?” Dr. Harman’s purpose for blogging ... basics of stem cell therapy so that pet owners can ... when considering regenerative medicine. , A veterinarian by trade, Dr. ...
(Date:7/10/2014)... 10, 2014  Franciscan St. Anthony Health-Crown ... for respiratory monitoring outside the operating room, ... in embracing state-of-the-art patient safety technology. ... breathing and can alert medical caregivers when ... amount of carbon dioxide the patient is ...
Breaking Biology Technology:Virtual Finger Enables Scientists To Navigate And Analyze 3D Images Of Complex Biological Structures 2Virtual Finger Enables Scientists To Navigate And Analyze 3D Images Of Complex Biological Structures 3DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 2DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 3DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 4DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 5DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 6Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 2Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 3Franciscan St. Anthony Health-Crown Point Underscores Patient Safety Commitment Through Expanded Use of Capnography 2
... SAN DIEGO, March 3, 2011 Anadys Pharmaceuticals, Inc. (Nasdaq: ... patient care by developing novel medicines for the treatment of ... for the fourth quarter and year ended December 31, 2010. ... well-positioned to create value in the evolving HCV landscape," said ...
... 2011 Novavax, Inc. (Nasdaq: NVAX ) ... visit the company,s headquarters in Rockville, Maryland, on Monday, ... at up to $179 million. The Office of Biomedical ... of the Assistant Secretary for Preparedness and Response at ...
... Executive search firm Sanford Rose Associates® - Minneapolis ... engagement for Nordson Corporation: Lise W. Duran as General ... of biomaterial applicators used in a wide variety of ... years of experience in the medical device industry, having ...
Cached Biology Technology:Anadys Pharmaceuticals Reports Fourth Quarter and Year-End 2010 Financial Results and Highlights 2Anadys Pharmaceuticals Reports Fourth Quarter and Year-End 2010 Financial Results and Highlights 3Anadys Pharmaceuticals Reports Fourth Quarter and Year-End 2010 Financial Results and Highlights 4Anadys Pharmaceuticals Reports Fourth Quarter and Year-End 2010 Financial Results and Highlights 5Anadys Pharmaceuticals Reports Fourth Quarter and Year-End 2010 Financial Results and Highlights 6Anadys Pharmaceuticals Reports Fourth Quarter and Year-End 2010 Financial Results and Highlights 7Maryland Governor Martin O'Malley to Visit Novavax in Celebration of HHS-BARDA Contract Award 2Maryland Governor Martin O'Malley to Visit Novavax in Celebration of HHS-BARDA Contract Award 3Maryland Governor Martin O'Malley to Visit Novavax in Celebration of HHS-BARDA Contract Award 4Sanford Rose Associates® - Minneapolis Fills Key Management Position for the Micromedics Operation of Nordson Corporation 2
(Date:7/10/2014)... , July 7, 2014 Fingerprint Cards, ... major design win (DW). A Chinese Top 5 smartphone OEM ... start of mass production in October 2014.   ... Top 5 smartphone OEM, which has a planned date for ... target sales volume for this phone of 3 million units. ...
(Date:7/10/2014)... GOTHENBURG, Sweden , July 8, 2014 ... FPC1021 has won a new design win (DW). An Asian ... targeted date for mass production start in August 2014.   ... an Asian smartphone OEM, which has a planned date for ... received an initial ramp order of SEK 5M, for delivery ...
(Date:7/10/2014)... Ill. , July 9, 2014 ... (EHR), spurred in large part by meaningful use ... health care providers interact with laboratory information. Now, ... in the field of clinical informatics in order ... To address the educational needs of ...
Breaking Biology News(10 mins):FPC Wins DW for 1021 Touch Sensor for Flagship Smartphone from Chinese Top 5 Smartphone OEM 2FPC Wins DW and Initial Ramp Order for FPC1021 Touch Sensor for Flagship Smartphone from Asian OEM 2New Clinical Informatics Curriculum Prepares Future Pathologists 2New Clinical Informatics Curriculum Prepares Future Pathologists 3New Clinical Informatics Curriculum Prepares Future Pathologists 4
... Marta Barrachina, Institute of Neuropathology of the Bellvitge Biomedical ... patients suffering from schizophrenia characterized by motor disorders. ... collaboration with the research team Mairena Martin at the ... clinical researchers of the Health Park Sant Joan de ...
... The Clinical Nutrition Research Centre (CNRC) -- a ... and Research (A*STAR) and National University Health Systems (NUHS) ... that will conduct nutritional studies to understand the causes ... develop products and formulate diets that can reduce the ...
... LAKE CITY, Utah, Jan. 29, 2014 Myriad Genetics, ... a clinical utility study with its Prolaris test, at ... Calif. The study demonstrated the significant clinical value ... prostate cancer. Prolaris is a prognostic test that ...
Cached Biology News:Identified a subgroup of schizophrenia patients with motor disorders 2Asia's first under-1-roof Nutritional Research Centre set up in Singapore 2Asia's first under-1-roof Nutritional Research Centre set up in Singapore 3Asia's first under-1-roof Nutritional Research Centre set up in Singapore 4Myriad's Prolaris significantly modifies treatment decisions for prostate cancer patients 2