Navigation Links
NIH 3T3

Multiporator Transfection Protocol Protocol No. 4308 915.021 11/1999 Cell line NIH 3T3, mouse embryo Transfection with Plasmid pEGFP-N1 (in bidistilled H2O) Electroporation buffer Eppendorf Hypoosmolar Electroporation Buffer (PH) Culture medium DMEM / 10% FCS Cuvette Eppendorf, 2 mm gap width, 400 l Temperature RT (20-25 C) Reference Dr. Rainer Pepperkok EMBL-Europisches Laboratorium fr Molekulare Biologie
Meyerhofstr. 1 D-69117 Heidelberg Phone +49 6221 387-332 Fax +49 6221 387-306
e-mail: pepperko@embl-heidelberg.de
  1. Harvest the cells in the exponential growth phase and centrifuge them (for 5-10 minutes, 200 x g, at room temperature).
  2. Resuspend the cells in DMEM / 0.5% FCS, determine the number of cells and centrifuge them (for 5-10 minutes, 200 x g, at room temperature). Remove supernatant.

    Note: The overall incubation time in the Eppendorf Electroporation Buffer must not exceed 30 minutes to guarantee a successful electroporation!

  3. Resuspend the cells in Hypoosmolar Electroporation Buffer. When doing so, set the cell concentration to 1 x 10
    '"/>


Source:


Page: All 1 2

Related biology technology :

1. Efficient Transfection of Neurospora Crassa
2. Efficient Transfection of Neurospora Crassa
3. Neurospora crassa
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: NIH

(Date:1/15/2014)... Look inside the new Preferred Solutions® ... lab, from fluid handling to instruments to supplies. Many ... you order. , Preferred Solutions features a full ... L/S® model for precise flow control and dispensing to ...
(Date:1/15/2014)... California , January 15, 2014 Oxford ... today announced the appointment of Thomas C Reynolds MD, PhD ... years, development experience gained in the biotechnology industry, most recently ... am delighted to welcome Tom at this transformative time for ...
(Date:1/15/2014)... 2014 This webinar will focus on ... safety assessment in biosimilars. , Regulatory frameworks are evolving ... development, however the complex nature of biopharmaceuticals makes the ... efficacy extremely challenging. Based on the specific aspects of ...
(Date:1/15/2014)... Houston, TX (PRWEB) January 15, 2014 ... Alzheimer’s disease, and about 1 in 3 seniors will ... ( source ). These jaw-dropping figures have shocked many ... function and, hopefully, help prevent these tragic age-related cognitive ...
Breaking Biology Technology:Cole-Parmer Begins 2014 with the Release of Preferred Solutions 2Oxford BioTherapeutics Appoints Thomas C Reynolds MD, PhD to its Board of Directors 2Oxford BioTherapeutics Appoints Thomas C Reynolds MD, PhD to its Board of Directors 3Xtalks Life Sciences Webinar Examines Safety Assessment of Biosimilars 2Biohack Pure Offers 5 Tips for Increasing Memory in 2014 2
... America,s Heartland, the,weekly public television series that celebrates the ... season this fall. Twenty-two new half-hour segments will ... middle of the third season,America,s Heartland will have profiled ... 50 states. The program is made possible with ...
... PALO ALTO, Calif., Oct. 3 Alexza,Pharmaceuticals, Inc. ... corporate,presentation will be webcast live during the 4th ... at 4:30 p.m. Eastern Time. The presentation,will be ... may be accessed,under the Investor Relations tab at ...
... to 45%, GURGAON, India, Oct. 3 Ranbaxy ... the,signing of Definitive Agreements, providing for an increase in ... at a price of Rs. 160 per share,aggregating to ... shares from the existing Promoters (b) Preferential Offer ...
Cached Biology Technology:America's Heartland Launches Third Season Highlighting Contributions of American Farmers and Ranchers 2The 4th Annual BIO Investor Forum to Webcast Alexza's Corporate Presentation 2Ranbaxy to Consolidate Position in Biopharmaceuticals and Speciality Injectables Including Oncology 2Ranbaxy to Consolidate Position in Biopharmaceuticals and Speciality Injectables Including Oncology 3
(Date:4/23/2014)... The most comprehensive study to date of the ... to more effective vaccine strategies and reveals surprising ... new results could alter public health strategies to ... worldwide each year. , Genomic analysis of 343 ... from around the world collected over the last ...
(Date:4/23/2014)... technique used to read quickly. It involves visual ... words and/ or sentences. Similarly to humans, biological ... "read" genetic information. Genes that need to be ... the encoding message, the easier it will be ... Gulbenkian de Cincia (IGC, Portugal) and Centre for ...
(Date:4/22/2014)... lead a five-year, $3 million study to determine whether ... transport of dissolved organic matter (DOM) through the Connecticut ... chemical composition and water quality of the watershed and ... Science Foundation,s MacroSystems Biology program, researchers will collect data ... in Canada and runs through five U.S. states, before ...
Breaking Biology News(10 mins):Impact of whooping cough vaccination revealed 2Cell division speed influences gene architecture 2Connecticut River watershed study will assess impacts of extreme rain events 2Connecticut River watershed study will assess impacts of extreme rain events 3
... of Illinois researchers identified the top pathogens, pests and ... Food Security. Soybean aphid, soybean rust, soybean cyst nematode, ... red leaf blotch, were featured as some of the ... and in the future. "Enormous potential exists to ...
... 2011) The remoteness and resource limitations of spaceflight ... solution is ultrasound. Scientists with the National ... expand the use of ultrasound during spaceflight and on ... include techniques that streamline training and help remote experts ...
... For most people, listening to the ocean means ... a sandy beach. But for researchers studying everything ... mapping to guiding robots trying to repair leaking undersea ... side underwater can reveal volumes of valuable ...
Cached Biology News:New and old threats to soybean production 2Space research gives birth to new ultrasound tools for health care in orbit, on Earth 2Space research gives birth to new ultrasound tools for health care in orbit, on Earth 3Space research gives birth to new ultrasound tools for health care in orbit, on Earth 4'Orca ears' inspire Stanford researchers to develop ultrasensitive undersea microphone 2'Orca ears' inspire Stanford researchers to develop ultrasensitive undersea microphone 3'Orca ears' inspire Stanford researchers to develop ultrasensitive undersea microphone 4
... SPD101B is a no-frills, basic SpeedVac concentrator ... economically priced, this unit still features a ... digitial display of time and heat. It ... and accepts a wide variety of different ...
... to have active, real-time sample temperature monitoring. ... the worlds first, fully modular SpeedVac System. ... together to form an integrated unit or ... also the first SpeedVac to have sample ...
Digital display of time, vacuum and temperature , Dual vacuum control of ramping rate and ultimate level , Comes complete with RH40-12 (40 x 1.5 ml) Rotor and (2) Glass Condensation Flasks. ...
... Acid Resistant SpeedVac Model SPD131DDA , ... , The SPD131DDA is designed to handle the ... a new slim design, digital display, dual independent ... TEFLON coated chamber and vapor pathway. , , ...
Biology Products: