Navigation Links
Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection,,,of Mammalian Cells


Mycoplasma PCR Primer Set for fast and easy detection of mycoplasma contamination

Leslie S. Arrington
Yale University

D. R. Staggs
Genetic Applications LLC

Heinz Miller
Stratagene

Mycoplasma contamination of mammalian cell cultures can substantially reduce the potency of transfection reagents.1 Cells must be free of mycoplasma contamination before the optimal amounts of LipoTAXI transfection reagent and DNA can be accurately determined. The use of Stratagenes Mycoplasma PCR Primer Set*, provides assurance that cells are free of mycoplasma contamination and able to maximally express transfected DNA.

The efficient introduction of foreign DNA into mammalian cells is vital to many areas of biological research. The ability to study the effect of introducing a gene or oligonucleotide of interest in a cell line may depend on the effectiveness of the transfection reagent. Stratagenes LipoTAXI transfection reagent can be used to achieve efficient transfections in a wide variety of mammalian cells. Using simple protocols, researchers can determine the optimal combination of LipoTAXI transfection reagent and plasmid DNA for a particular cell type.2 However, mycoplasma contamination can interfere with transfection using lipid, DEAE-dextran, CaPO4 and adenovirus-mediated methods.1 The resulting low transfection efficiencies may go undetected since antibiotic-resistant mycoplasma can exist in cell cultures without noticeable visual effects on the cells.3

In the typical protocol for optimizing transfection conditions, the amount of LipoTAXI reagent is increased at various concentrations of plasmid DNA that encodes for a reporter gene. Expression of the reporter
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. A New PCR-based Mycoplasma Detection Method
2. Preventing Carry-over Contamination with Uracil-DNA Glycosylase
3. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
4. Combat RNase Contamination in the Lab
5. The Fastest, Simplest, and Most Effective Way to Remove DNA Contamination
6. Testing Compressed Air Lines for Microbiological Contamination
7. Testing Compressed Air Lines for Microbiological Contamination
8. Effects of Thermal Cycler Well-To-Well Temperature Uniformity on PCR Results
9. Effectene Transfection Reagent provides efficient gene delivery to primary neuronal cell cultures
10. Effectene Transfection Reagent
11. Measuring Gene Silencing Effects by RT-PCR Without RNA Isolation
Post Your Comments:
(Date:8/20/2014)... , Aug. 20, 2014 /PRNewswire-iReach/ -- A ... transformative collaboration between the school,s bioengineering department and ... of the Intel® Software Academic Program, UCSD,s research ... and the human body.  Photo ... study exhibits the work of Dr. Todd ...
(Date:8/20/2014)... 2014 GraphDB™ 6.0 ... improvements to the enterprise replication cluster, faster loading ... SOLR and Elasticsearch. This release happens to coincide ... triplestore – GraphDB™ was formerly known as OWLIM. ... only mature enterprise resilient RDF triplestore will also ...
(Date:8/19/2014)... Research and Markets  has announced the addition of the ... report to their offering. This report analyzes the ... by the following Product Segments: Media, Sera, and Reagents. ... Canada , Japan , Europe ... America . Annual estimates and forecasts are provided for the ...
(Date:8/19/2014)... DUBLIN , Aug. 19, 2014 Research ... Oxide Fuel Cells (SOFCs) - Global Strategic Business Report" ... the worldwide markets for Solid Oxide Fuel Cells (SOFCs) in ... US, Canada , Europe ... Annual estimates and forecasts are provided for the period 2012 ...
Breaking Biology Technology:Intel and University of San Diego Bioengineering Department Release Case Study on Health Sciences Research 2Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 2Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 3Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 4Global Cell and Tissue Culture Supplies - Strategic Business Report 2014 2Global Solid Oxide Fuel Cells (SOFCs) - Strategic Business Report 2014 2
... game of inches," but biology is a game of nanometers, ... the fate of a cell - whether it lives or ... Department of Energy (DOE),s Lawrence Berkeley National Laboratory (Berkeley Lab) ... impact of spatial patterns on living cells. Chemist Jay ...
... GAITHERSBURG, Maryland, November 2, 2011 - ... -based HPV Testing Increases Screening Coverage and is More Sensitive ... in the Lancet Promising findings published today ... community-based, randomized equivalence trial undertaken by public health authorities in ...
... Epitomics, Inc., a California based biotech company, announced ... Allowance from the United States Patent and Trademark ... Proteins (application 10/705,109).  The Notice allowed 17 claims ... samples using libraries of antibodies.  This method has ...
Cached Biology Technology:Berkeley Lab scientists develop new tool for the study of spatial patterns in living cells 2Berkeley Lab scientists develop new tool for the study of spatial patterns in living cells 3Home-based Self-collected Vaginal Specimens Detect More Cervical Lesions and Cancers Than Cytology 2Home-based Self-collected Vaginal Specimens Detect More Cervical Lesions and Cancers Than Cytology 3Home-based Self-collected Vaginal Specimens Detect More Cervical Lesions and Cancers Than Cytology 4Home-based Self-collected Vaginal Specimens Detect More Cervical Lesions and Cancers Than Cytology 5
(Date:8/19/2014)... Some infants born with neonatal abstinence syndrome ... more difficult time going through withdrawal than others, ... While genetic and epigenetic (when genes are turned ... as potential factors, researchers at Boston University School ... conducted a first of its kind study to ...
(Date:8/19/2014)... students in college biology and other science courses ... quantitative ways, and now they have a new ... the Life Sciences," published this month by Princeton ... Institute for Mathematical and Biological Synthesis (NIMBioS), teaches ... can be used to explore and explain biological ...
(Date:8/19/2014)... of UTSA researchers has been awarded a nearly $40,000 ... Training (NCPTT), an office of the National Park Service ... will fund a one-year study of the energy efficiency ... homes in hot, humid climates. , Radiant barriers are ... that reflect heat back, thereby reducing the amount of ...
Breaking Biology News(10 mins):In-utero methadone, Subutex exposure could alter gene expression, cause severe Neonatal Abstience Syndrome 2New textbook introduces undergraduates to mathematics for the life sciences 2UTSA researcher awarded $38K+ to evaluate energy efficiency options for historic homes 2UTSA researcher awarded $38K+ to evaluate energy efficiency options for historic homes 3
... certain disease mechanisms, illness progression and response to new ... such as nematodes, fruit flies or zebra fish. ... of many individual animals to detect mutants worthy of ... system that uses artificial intelligence and cutting-edge image processing ...
... scientists at the University of California, Berkeley, provide a clearer ... change, and where they go. One study, published ... Biology , finds that changes in precipitation have been underappreciated ... normal range. In the other study, published today (Wednesday, Aug. ...
... Mich. --- Two new species of owls have been discovered ... a key role in confirming their existence. The ... Forktail, the Journal of Asian Ornithology , took years to ... paper,s lead author Pam Rasmussen, MSU assistant professor of zoology ...
Cached Biology News:Artificial intelligence helps detect subtle differences in mutant worms 2Artificial intelligence helps detect subtle differences in mutant worms 3Artificial intelligence helps detect subtle differences in mutant worms 4Studies shed light on why species stay or go in response to climate change 2Studies shed light on why species stay or go in response to climate change 3Studies shed light on why species stay or go in response to climate change 42 new owls discovered in the Philippines 2
...
Experion StdSens chips are the microfluidic chips used to perform standard-sensitivity RNA analysis (nanogram levels) with the Experion automated electrophoresis system. Each chip has the capacity to...
Experion HighSens chips are the microfluidic chips used to perform high-sensitivity RNA analysis (picogram levels) with the Experion automated electrophoresis system. Each chip has the capacity to ru...
...
Biology Products: