Navigation Links
Mycobacterium tuberculosis

Multiporator / Electroporator 2510 Transformation Protocol Protocol No. 4308 915.524 03/2002 Microorganism Mycobacterium tuberculosis H37Rv Cell type Bacteria, gram positive Molecules injected Plasmid DNA Growth medium Middlebrook 7H9 medium with 0.2% glycerol, 0.25% Tween 80, albumin-dextrose complex (ADC) Washing solution 10% glycerol Electroporation solution 10% glycerol Outgrowth medium Middlebrook 7H9 medium with ADC, selective 7H10 agar plates with ADC Cuvette 2 mm gap width Reference Armitige, L. Y. et al 2000 Infection and Immunity 68, No. 2 767-778 Making electrocompetent cells:

1. Grow cells with gentle shaking to an OD600 of 0.6-1.0. 2. Harvest cells and wash three times in 1/50 volume of cold 10% glycerol. 3. Resuspend in 10% glycerol at a concentration of approx. 1011 cells/ml and store at 70 C until needed.

Electroporation of cells:


'"/>

Source:


Page: All 1 2

Related biology technology :

1. Mycobacterium avium
2. Mycobacterium intracellulare
3. Mycobacterium smegmatis
4. Mycobacterium avium
5. Mycobacterium intracellulare
6. Mycobacterium smegmatis
7. Mycobacterium tuberculosis
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Mycobacterium tuberculosis

(Date:7/23/2014)... , July 23, 2014 /PRNewswire/ - BioAmber ... biotechnology company producing sustainable chemicals, today announced that the ... full their option to purchase an additional 420,000 ... price of $12.00 per share, less underwriting discounts ... underwritten public offering of 2,800,000 shares of common ...
(Date:7/23/2014)... MENLO PARK, Calif. , July 23, 2014 /PRNewswire/ ... based investment firm, today announced the closing of Sofinnova ... $500 million and in excess of the initial fund ... on helping entrepreneurs build successful biotechnology companies. Consistent with ... companies with promising later stage clinical programs, along with ...
(Date:7/23/2014)... a2z Inc. announces that AACC will ... Pathfinder, in its 2014 Annual Meeting & Clinical ... planned for July 29-31, 2014 in Chicago, IL at ... connect with global leaders in clinical chemistry, molecular diagnostics, ... of breaking science in laboratory medicine. , ChirpE Photo ...
(Date:7/22/2014)... 2014 The Board of Directors of BD (Becton, ... a quarterly dividend of 54.5 cents per common share payable ... 9, 2014. The indicated annual dividend rate is $2.18 per ... a leading medical technology company that partners with customers and ... evolving health needs. Our innovative solutions are focused on improving ...
Breaking Biology Technology:BioAmber announces exercise in full of underwriters' option in connection with previously priced follow-on offering 2BioAmber announces exercise in full of underwriters' option in connection with previously priced follow-on offering 3Sofinnova Raises $500 Million Biotech Venture Fund 2AACC Enhances Attendee Experience at Its 2014 Annual Meeting & Clinical Lab Expo with a2z-Powered ChirpE Photo Booth and Mobile App 2AACC Enhances Attendee Experience at Its 2014 Annual Meeting & Clinical Lab Expo with a2z-Powered ChirpE Photo Booth and Mobile App 3
... IRVINE, California and KARLSRUHE, Germany , June 16, 2010 ,The ... and the US. "Growth requires space" had already been the motto of,the last company ... ... is moving from Campbell to Irvine, California , one,of high-tech industry ...
... N.M. The world,s smallest chess board about ... pea-sized microbarbershop were winners in this year,s design contest ... at Sandia National Laboratories in mid May. The ... Sandia,s microfabrication facility, one of the most advanced in ...
... Raju Kucherlapati , Professor of Genetics at Harvard ... making—will be addressing the Controlled Release Society on the subject of ... , , ... diagnosed with non small cell lung cancer before 2003, there were ...
Cached Biology Technology:joimax(R) Moves Into Larger Premises 2Texas Tech, U of Utah win Sandia microdevice competition 2Texas Tech, U of Utah win Sandia microdevice competition 3Texas Tech, U of Utah win Sandia microdevice competition 4Personalized Treatments for Cancer Are Revolutionizing Patient Care 2Personalized Treatments for Cancer Are Revolutionizing Patient Care 3
(Date:7/23/2014)... , , , , , ... , , , , , ... changing how medical care is delivered could help prevent multiple chronic diseases and extend healthy lifespan. The idea, they argue, would be to target the... ... , , , , ... , , , , , ...
(Date:7/23/2014)... grip is affecting more than the color of people,s lawns. ... heat-loving amoeba commonly found in warm freshwater bodies, such as ... warmer than usual this year. , A 9-year-old Kansas girl ... swimming in several area lakes. The amoeba enters the body ... brain. Nose plugs can lower the odds of this rare ...
(Date:7/23/2014)... planet,s soil releases about 60 billion tons of carbon ... than that released by burning fossil fuels. This happens ... of carbon is balanced by carbon coming into the ... as well as by the underground activities of plant ... rising temperatures increase the rate of soil respiration. As ...
Breaking Biology News(10 mins):Strategy proposed for preventing diseases of aging 2Strategy proposed for preventing diseases of aging 3Strategy proposed for preventing diseases of aging 4Strategy proposed for preventing diseases of aging 5Diseases of another kind 2Climate change and the soil 2
... of our hearts and blood vessels. Conventional treatment attempts ... in the blood plasma. The opposite approach, which involves ... acid, has proven unpopular among patients up to now. ... acid has an unpleasant but harmless side-effect: the ...
... November, the Malaspina expedition 2010 will set sail from ... the Spanish National Research Council (CSIC) aims to evaluate ... to survey their biodiversity. The expedition has the backing ... and takes its name from mariner Alessandro Malaspina, the ...
... MADISON, WI, July 26th, 2010 NASA scientists ... and ground-based observations of precipitation. The NASA data ... to monitor non-point pollution and improve water quality. ... of Science Systems and Applications, Inc., incorporated two ...
Cached Biology News:Preventing heart problems while keeping a cool head 2Preventing heart problems while keeping a cool head 3Malaspina expedition 2010 2NASA satellite improves pollution monitoring 2
Request Info...
... combines deep experience, skills and flexibility in ... team chemistry. This combination enables optimum solutions ... beyond.,With AAIBiotech, you can count on our ... than that: its the power of team ...
ZBP-89 (S-15)...
... Physical form: Lyophilized ... salts Preparation Unit Definition: ... 1.0μmole of 2-phosphoglycerate to ... pH7.4 at 25 C. ...
Biology Products: