Navigation Links
Mycobacterium avium

Multiporator / Electroporator 2510 Transformation Protocol Protocol No. 4308 915.523 03/2002 Microorganism Mycobacterium avium Cell type Bacteria, gram positive Molecules injected Plasmid DNA Growth medium Middlebrook 7H9 medium with 0.1% Tween and Dubos oleic albumin complex
enrichment Washing solution 10% glycerol Electroporation solution 10% glycerol Outgrowth medium Middlebrook 7H9 liquid medium with 0.1% Tween and Dubos oleic albumin complex
enrichment, selective Middlebrook 7H10 agar plates Cuvette 1 mm gap width Reference Beggs, M. L. et al 1995 Journal of Bacteriology 177, No. 17 4836-4840 Making electrocompetent cells:

1. Grow cells in sidearm flask or tissue culture plates with gentle agitation until a Klett unit reading of 100 to 200 was
obtained. 2. Harvest cells and wash three times with cold sterile 10% glycerol. 3. Resuspend 1/100 volume of 10% glycerol.

Electroporation of cells:

  1. Add up to 1 g plasmid DNA to 60 l of electrocompetent cells. Homogenize by gently mixing with pipette several
    times. Incubate on ice for 10 min. Transfer mixture into a prechilled cuvette.
  2. Wipe moisture from the cuvette and insert the cuvette into the device.
  3. Electroporation:

    Mode Prokaryotes O Voltage (V) 1,200 V Time constant (T) 5 ms
  4. Wash cells out of the cuvette with 1 ml of outgrowth medium and incubate for 2 h at 37 C.
  5. Plate on selective agar.
Expected Results: Transformation efficiency up to 105 transformants/g of DNA.



Page: All 1 2

Related biology technology :

1. Mycobacterium avium
2. Mycobacterium intracellulare
3. Mycobacterium smegmatis
4. Mycobacterium tuberculosis
5. Mycobacterium intracellulare
6. Mycobacterium smegmatis
7. Mycobacterium tuberculosis
Post Your Comments:

(Date:10/12/2015)... Oct. 12, 2015 /PRNewswire/ - Aeterna Zentaris Inc. (NASDAQ: ... engaged in developing and commercializing novel treatments in oncology, ... Dennis Turpin , the Company,s former Senior Vice ... its Quebec City office.  ... Chief Executive Officer of the Company commented, "After a ...
(Date:10/12/2015)... PHILADELPHIA , October 12, 2015 ... alternative to print version for enriched online experience ... media alternative to print version for enriched online ... offers user-friendly, multi media alternative to print version ... a world-leading provider of scientific, technical and medical information ...
(Date:10/12/2015)... ... , ... NeuMedics Inc., is a specialty biopharmaceutical company focused on identifying and ... be administered as an eye drop, announced today it has been selected to present ... place October 25th to October 28th at The Cleveland Clinic, Cleveland, Ohio. The ...
(Date:10/10/2015)... , le 10 octobre 2015 Le 8 octobre, ... intégré dans le Registre du Congrès sa déclaration ... pour la sensibilisation autour du plasma (IPAW), qui ... sponsorisée par la Plasma Protein Therapeutics Association ... à : , Sensibiliser l,opinion publique ...
Breaking Biology Technology:
(Date:10/8/2015)... Connecticut , October 8, 2015 ... ("NXT-ID" or the "Company"), a biometric authentication company ... creator of the Wocket® smart wallet announces that ... 2015 were approximately $410,000 compared with $113,00 for ... for the 9 months ended September 30, 2015 ...
(Date:10/6/2015)... , Oct. 6, 2015 Track Group, Inc. ... that it has signed a contract with the Virginia ... the full range of sentences under the Department,s oversight. ... the Americas. "This contract with the Virginia DOC will ... US and advances our position as a trusted leader ...
(Date:10/2/2015)... Oct. 02 2015 ... "Enforcing the Law Using Biometrics" report ... has announced the addition of the "Enforcing ... offering. --> Research and Markets ( ... "Enforcing the Law Using Biometrics" report ...
Breaking Biology News(10 mins):