New System for Improving QPCR
Stratagene has improved upon currently available QPCR machines with the Mx4000 multiplex quantitative PCR system. This new system offers better performance and functionality at a more economical price.
Real-time quantitative PCR (QPCR) allows researchers to quickly and easily quantify nucleic acids for studying gene expression, mutational analysis, disease state, and gene dosage. QPCR measures PCR product accumulation during the exponential phase of the reaction using fluorescence data and provides accurate information on initial starting copy number. PCR amplification and detection are combined in a single step, speeding the process of gathering experimental data. The QPCR process uses a single closed tube, which eliminates the need for numerous post-PCR manual steps, and reduces the possibility of introducing variability or laboratory contamination.
The Mx4000 system detects multiple fluorescent PCR chemistries and combines the capabilities of a microplate fluorescence reader with a PCR thermal cycler into a single real-time detection system. The system includes an integrated, high-performance, proprietary thermal system and a multiple-fluorophore excitation and emission detection system. Applications include allelic discrimination, plate reads, melting curves, and quantification of single or multiplexed targets.
Size Comparison of Mx4000 and ABI Prism Systems
The Mx4000 multiplex quantitative PCR system has a detection range of 350 nm to 830 nm, allowing greater flexibility of fluorophore choice, as well as providing high sensitivity and excellent signal-to-noise ratio. The Mx4000 systems light source generates an extended excitation range from 350 to 750 nm. This allows researchers to choose fluorophores with little or no spectral overlap, to produce clean, delineated signals for superior multiplexing. Each of the four scanning fiber-optic heads independently excites and detects dyes, reading up to four dyes in a single tube.
Obtain Better Discrimination of Dyes
Optimized interference filters precisely match the excitation and emission wavelengths for each fluorophore to block out unwanted cross-talk from spectrally adjacent fluorophores. Researchers can choose from FAM, HEX, TAMRA, Cy5, Cy3, Texas Red/ROX, and TET filter sets. Custom filter sets are also available for other fluorophores.
Detect Up to Four Fluorophores in the Same Tube
The Mx4000 system software increases your options with new features such as real-time amplification plots that can be viewed as your PCR progresses. It makes many applications easier than ever before, such as real-time and plate-read allelic discrimination, real-time QPCR, qualitative detection, and melting curve profiles.
The advanced software offers many competitive features, including real-time amplification plots as the run progresses. This allows you to determine at a glance how an experiment is running at any time during thermal cycling, rather than waiting until the end of the run. You can choose to abort a run if a problem develops in a reaction, or stop the experiment and save the data as soon as the desired information is generated.
Plots as PCR Progresses
The software also features the ability to make allelic discrimination calls by endpoint fluorescence values, as well as by threshold cycle. Data can be viewed in many forms including amplification plots, scatter plots, sample value screens for the entire plate for all dyes, fluorescence intensity screen, final call results, melting curves, annealing range, and text reports.
A time-saving feature allows you to select a subset of the 96 wells for independent analysis, allowing you to perform multiple sub-experiments on a single plate.
The thermal system delivers unsurpassed coupling of the thermal control system to the sample, using patent-pending solid-state heating and cooling technology. This new technology delivers superior thermal ramp rates, unparalleled thermal accuracy, and the best temperature uniformity in a 96-well plate format. The extremely low thermal mass of the sample thermal control block promotes rapid changes in temperature, so your experiments are completed in less time. A typical 96-sample quantitative PCR thermal profile of 40 cycles can be completed in less than 90 minutes.
The software establishes robust communication between the instrument and the attached personal computer. Even in the event of a po wer loss to the PC or communications error, data collection continues, and when communication is restored, the data from the run is transferred from the instruments embedded software to the software on the PC, ensuring that the experiment will be completed and data successfully saved.
* Patent pending