( heterozygousat either position 316 o...)Mutation detection for the,,,K- rasand P16 genes

heterozygous at either position 316 or 356. The CG mutation at position 316 produced one extra band. However, when a sample displayed the CG mutation at position 316 and a CT mutation at position 356, two extra bands could be seen. The extra bands detected here are due to the slower mobility of the heteroduplex formed by heterozygote mutant of the samples. The detection on the Agilent 2100 bioanalyzer are

completely matched with our genotyping results using ABIs 3700 sequencer. Figure 3 shows hetero-zygous mutations and double homozygous mutations. Figure 4 shows the DNA sequence chromatogram data which corresponds to the Agilent data generated. This size range resolution could not be visualized on a slab gel.


Conclusion
The data obtained from our studies show how the Agilent 2100 bioanalyzer can distinguish between mutated and normal DNA samples in particular genes. This is extremely important in making a genetic diagnosis in cancer patients. This makes for a rapid and accurate screening assay, which can be employed by any molecular biology laboratory. These findings suggest that the Agilent 2100 bioanalyzer could be used for some SNP detection assays.



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