Navigation Links
Mutation detection for the,,,K- rasand P16 genes

as above, but for 40 cycles. Again, the amplicon was purified and subjected to the same restriction enzyme digest/purification. At this point the samples were run on the Agilent 2100 bioanalyzer and submitted for DNA sequencing. For the P16 gene, the final reaction concentrations were 0.1 M of each primer4 (Invitrogen), with 0.2 mM dNTPs (Invitrogen), 1 unit of Platinum Taq High Fidelity, 1X PCR buffer (Invitrogen), 3 mM magnesium sulfate, 3 % DMSO (Sigma), 10 ng of DNA template, and HPLC-grade water (Fisher) in a total volume of 50 l. An initial 3-minute denaturation at 95 C was followed by 40 cycles at the following conditions, again on ABIs 9700 thermal cycler: 95 C for one minute, 58 C for 1 minute, and 72 C for 1 minute. A ten minute final extension at 72 C was also incorporated. Amplicons were freed of excess primers and nucleotides using ExoSap (USB), analyzed on the Agilent 2100 bioanalyzer, and submitted for DNA sequencing. Sequencing reactions were purified/de-salted using Sephadex G50 (Sigma).
Analysis of PCR-products
Amplified products were electrophoresed on Agilents 2100 bioanalyzer. The DNA 1000 LabChip kit was used in accordance with manufacturers instructions. Briefly, 9 l of the gel dye mixture was added to the chip well labeled G. This was pressurized for one minute throughout the chip with the syringe attachment provided. Then 9 l of the gel dye mixture was added to the other two chip wells labeled with G. 1 l of ladder was added to the ladder well, followed by 5 l of the gel dye mixture. This was pipetted up and down several times to mix. 5 l of the markers were added to each of the twelve sample wells. 1 l of each sample was added to their corresponding wells on the chip. The chip was vortexed for one minute on the IKA vortex adapter provided at the recommended setting. The chip was
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
2. Organize Your Data from the Big Blue Mutation Assay
3. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
4. Tissue Specificity for Mutation Parallels Tissue Specificity for Cancer
5. A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis
6. Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System
7. Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis
8. Identification of Disease Causing Mutations in Phenylketonuria by Denaturing Gradient Gel Electrophoresis Using the DCode System
9. Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations
10. Detection of Mutations in the CYP21 Gene Using the DCode System
11. Rapid, High Throughput Mutation Discovery Using Fluorescent SSCP on the ABI PRISM 3100 and 3100Avant Genetic Analyzers
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Mutation detection for the rasand genes

(Date:1/15/2014)... 2013 was a banner year of ... They saw continued independent research led by the team ... a $1 million grant from the Susanne Marcus Collins ... a peer reviewed journal, Amy Grant highlighted Brainwave Optimization® ...
(Date:1/15/2014)... , Jan. 15, 2014 TaiGen Biotechnology Company, Limited ... agreement with R-Pharm, a leading Russian pharmaceutical company, to ... Russian Federation , Turkey ... States (CIS). Nemonoxacin is a novel antibiotic for the treatment ...
(Date:1/14/2014)... 14, 2014 Histogen, Inc., a regenerative ... cells grown under simulated embryonic conditions, today announced that ... Suneva Medical, Inc. for physician-dispensed aesthetic products containing Histogen’s ... This agreement is an amendment to the existing license ...
(Date:1/14/2014)... NY (PRWEB) January 14, 2014 EquitiesIQ, ... Alliqua, Inc. (OTCQB: ALQA). Alliqua is an emerging biomedical ... serve the wound care market. , Free report download: ... restructured with a seasoned management team and Board, which ...
Breaking Biology Technology:Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 2Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 2TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 4Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 2Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 3EquitiesIQ Initiates Coverage of Alliqua, Inc. 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 3
... Associates has announced the development of the Core Communications Program, ... investors, collaborators, and customers. , ... ... http://www.mainsgate.com [Mains Associates] has announced the availability of ...
... electronic properties, few layer graphene, or FLG, has emerged ... devices that incorporate the quantum effects that emerge at ... Pennsylvania have demonstrated a new method by which FLG ... thermally activated nanoparticles, a technique that results in atomically ...
... (Nasdaq: ONXX ) today announced that it ... Pharmaceuticals Conference on,Thursday, August 7, at 3:05 p.m. ... of the presentation on our website at:, http://www.onyx-pharm.com/wt/page/event_calendar ... on 15 minutes early in order to,register and ...
Cached Biology Technology:Bay-Area Company Gives Science-Based Startups a Voice 2Penn scientists carve functional nanoribbons using super-heated, nano-sized particles of iron 2
(Date:7/9/2014)... announce themselves with pain and fever but often can be ... are sneaky and hard to beat. Now, scientists have built ... tiny DNA pyramids. Published in the journal ACS Applied ... flag bacteria and kill more of them than medicine alone. ... infectious pathogens can lie in wait, undetectable in the human ...
(Date:7/9/2014)... developed by researchers at the NIH,s National ... its collaborators to treat sickle cell disease ... business. The drug candidate, Aes-103, is the ... molecular mechanism of sickle cell disease. Baxter ... required for regulatory approval and commercialization. , ...
(Date:7/9/2014)... cancer patients with high levels of vitamin D in their ... shows. , Patients with the highest levels of vitamin ... with the lowest levels, the findings reveal. , The study ... D in bowel cancer patients after their diagnosis which ... from dietary sources with their long term survival prospects. ...
Breaking Biology News(10 mins):First drug candidate from NIH program acquired by biopharmaceutical company 2First drug candidate from NIH program acquired by biopharmaceutical company 3First drug candidate from NIH program acquired by biopharmaceutical company 4
... BioScience includes the following peer-reviewed research articles: ... , Yufeng Wang and Timothy G. Lilburn. , The ... in resource collections that are well characterized with the ... sequence data, using ideas from systems biology. ...
... Institute of Harvard and MIT and Beth Israel Deaconess ... previously unrecognized mammalian genes that do not encode proteins, ... presented in the February 1st advance online issue of ... class of "large intervening non-coding RNAs" or "lincRNAs" plays ...
... Louis, MO, USA, February 1, 2009 A special ... Journal of the American Dietetic Association presents findings ... Study (SNDA-III), conducted by Mathematica Policy Research, Inc., as ... Sponsored by the Food and Nutrition Service of the ...
Cached Biology News:BioScience tip sheet, February 2009 2Scientists uncover new class of non-protein coding genes in mammals with key functions 2Scientists uncover new class of non-protein coding genes in mammals with key functions 3Results of the third school nutrition dietary assessment study published 2Results of the third school nutrition dietary assessment study published 3Results of the third school nutrition dietary assessment study published 4
The Trans-Blot Plus gel and cassette assembly tray is used to hold the Trans-Blot Plus gel holder cassette, buffer, and other materials for the gel and membrane sandwich during their assembly....
Criterion staining and blotting trays are plastic trays designed for staining one or two Criterion gels or performing western blot detection. The trays have a working capacity of up to 500 ml. Suppli...
... range of next-generation features into a small-footprint ... for flexible laboratory solutions. It puts every ... dilution, dispensing, and integrations into a ... flexible as it is efficient and economical. ...
... FX P is the latest entry in ... greater positional accuracy and increased robustness, it can ... It can be configured with either one or ... the Biomek FX P sets the standard ...
Biology Products: