Navigation Links
Mutation detection for the,,,K- rasand P16 genes

also viewed on the chip before wasting the reagents and time to sequence it. Often formalin-fixed tissues or lymph node metastases do not present good quality DNA template for PCR reactions. The chip images were a qualitative as well as a quantitative tool that indicated if a sample needed to be repeated to increase its yield. The resolution was also better on the Agilent 2100 bioanalyzer platform than on ethidium-stained agarose gels. Chips were safer to work with and preparation time was reduced dramatically.
Materials and methods
Tissues
Sample blocks were obtained through the National Cancer Institutes Naval Medical Oncology Branch. Ten 5-m sections were deparaffinized and extracted using the Series III kit from Xtrana. For P16, DNA was received already extracted in barcoded 96- well plates. We quantitated the DNA via spectrophotometry and it was diluted to 10 ng for starting material.
PCR
Nested PCR was performed for K-ras with final reaction concentrations of 0.25 M each primer3 (Invitrogen), 0.2 mM dNTPs (Roche), 1X PCR buffer (Roche), 1.25 U Taq polymerase (Roche), HPLC-grade water (Fisher), and Amp Enhancer (Xtrana). 50 l were added to the bound DNA on the Xtrana tubes. For samples not extracted using the Xtrana kit, the Amp Enhance solution was eliminated and solubilized template was incorporated. This reaction was cycled 18 times using ABIs 9700 thermal cycler under the following conditions: 94 C for 45 seconds, 55 C for 1 minute, and 72 C for 90 seconds. This was purified using the Wizard kit (Promega). The eluant was used as template in a BstNI reaction with BSA (New England BioLabs). This reaction was purified using the MinElute kit (Qiagen). Then an aliquot was used in the second round of PCR; the same conditions
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
2. Organize Your Data from the Big Blue Mutation Assay
3. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
4. Tissue Specificity for Mutation Parallels Tissue Specificity for Cancer
5. A Multiple Mutation Model System as a Test Development and Training Tool for Denaturing Gradient Gel Electrophoresis
6. Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System
7. Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis
8. Identification of Disease Causing Mutations in Phenylketonuria by Denaturing Gradient Gel Electrophoresis Using the DCode System
9. Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations
10. Detection of Mutations in the CYP21 Gene Using the DCode System
11. Rapid, High Throughput Mutation Discovery Using Fluorescent SSCP on the ABI PRISM 3100 and 3100Avant Genetic Analyzers
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Mutation detection for the rasand genes

(Date:7/2/2015)... July 2, 2015  Spherix Incorporated (Nasdaq: SPEX ... and monetization of intellectual property, today announced a litigation ... Incorporated v. Verizon Services Corp. et al. , Case ... the Eastern District of Virginia , ... 2015 interpreting certain key claims in favor of the ...
(Date:7/2/2015)... Memphis, TN (PRWEB) , ... July 02, 2015 ... ... in the development and manufacturing of surgical instruments in Orthopedics, announced today the ... orthopedic contract-manufacturing and the world's largest provider of surgical instruments to the Spine ...
(Date:7/1/2015)... 1, 2015   Decision Resources Group ... of Otezla are its oral administration, alternative mechanism ... by surveyed rheumatologists. Otezla was the first oral ... of active psoriatic arthritis in the ... care includes conventional oral treatments including disease-modifying antirheumatic ...
(Date:7/1/2015)... 1, 2015   Neuraltus Pharmaceuticals, Inc. , a ... groundbreaking drugs to treat neurodegenerative diseases such as ... Robert G. Miller , M.D., Director of the Forbes ... Pacific Medical Center in San Francisco, has been awarded ... million to help fund a Phase 2 clinical ...
Breaking Biology Technology:Spherix Issues A Litigation Update 2Spherix Issues A Litigation Update 3In'Tech Medical Acquires Turner Medical to Become a World Leader in Orthopedic Contract-Manufacturing 2In'Tech Medical Acquires Turner Medical to Become a World Leader in Orthopedic Contract-Manufacturing 3Surveyed Rheumatologists Report That Otezla's Oral Administration is its Single Greatest Advantage Over Currently Available Treatment Options 2Surveyed Rheumatologists Report That Otezla's Oral Administration is its Single Greatest Advantage Over Currently Available Treatment Options 3Investigator to Receive $1.5 Million Grant from the ALS Association to Help Fund Confirmatory Phase 2 Study of Neuraltus Pharmaceuticals' NP001 2Investigator to Receive $1.5 Million Grant from the ALS Association to Help Fund Confirmatory Phase 2 Study of Neuraltus Pharmaceuticals' NP001 3Investigator to Receive $1.5 Million Grant from the ALS Association to Help Fund Confirmatory Phase 2 Study of Neuraltus Pharmaceuticals' NP001 4
... Nile Therapeutics, Inc. (Nasdaq: NLTX ), a company focused ... announced that it has completed the dose escalation stage of an ... heart failure.  Nile had previously announced that the study, NIL-CDNP-CT005, was ... Phase II studies. , , ...
... May 7, 2010 HOYA Corporation today,announced financial results for the fourth ... 104.8 billion yen for the quarter,representing an increase of 26.4% from ... recovery in orders in the main business fields,such as Electro-Optics, as well as ... , ...
... the Swedish medical university Karolinska Institutet shows that the ... cholesterol and thereby cause an inflammation that leads to ... receptors, the researchers have managed to inhibit the development ... in the distinguished periodical Journal of Experimental Medicine ...
Cached Biology Technology:Nile Therapeutics Completes Dose Escalation in Ongoing Phase II Study 2Nile Therapeutics Completes Dose Escalation in Ongoing Phase II Study 3Hoya Announces Fourth Quarter and Full-Year Financial Results 2Hoya Announces Fourth Quarter and Full-Year Financial Results 3New atherosclerosis vaccine gives promising results 2
(Date:6/24/2015)... 24, 2015 This report provides market analysis ... six years. It contains an analysis of the drivers, ... their impact from the short, medium, and long term ... industry, market, and technology trends that currently, prevail in ... concerned authorities to efficiently manage the increasing marine traffic ...
(Date:6/23/2015)... , June 23, 2015   MedNet ... that supports the entire spectrum of clinical research, ... ™ , the company,s intuitive, flexible ... awarded a Silver 2015 Stevie® Award by the ... Products & Services Website category.  The American Business ...
(Date:6/18/2015)... June 18, 2015 This report analyzes the ... the following Product Segments, which includes Immunoassay Analyzers and ... Systems, Radio Immunoassay Systems, and Nephelometric Immunoassay Systems. The ... Canada , Japan , ... , Latin America , and Rest ...
Breaking Biology News(10 mins):Automatic Identification System Market by Class, by Platform, by Application and by Geography - Global Forecasts & Analysis to 2014 - 2020 2MedNet Solutions' iMedNet eClinical Wins Silver Stevie Award 2Global Immunoassay Systems Market 2Global Immunoassay Systems Market 3Global Immunoassay Systems Market 4Global Immunoassay Systems Market 5Global Immunoassay Systems Market 6Global Immunoassay Systems Market 7Global Immunoassay Systems Market 8Global Immunoassay Systems Market 9Global Immunoassay Systems Market 10Global Immunoassay Systems Market 11Global Immunoassay Systems Market 12Global Immunoassay Systems Market 13Global Immunoassay Systems Market 14Global Immunoassay Systems Market 15Global Immunoassay Systems Market 16Global Immunoassay Systems Market 17Global Immunoassay Systems Market 18Global Immunoassay Systems Market 19Global Immunoassay Systems Market 20Global Immunoassay Systems Market 21Global Immunoassay Systems Market 22Global Immunoassay Systems Market 23Global Immunoassay Systems Market 24Global Immunoassay Systems Market 25Global Immunoassay Systems Market 26Global Immunoassay Systems Market 27Global Immunoassay Systems Market 28Global Immunoassay Systems Market 29
... 14, 2011 - Eurofins MWG Operon, an expert in next ... genomic DNA libraries for sequencing with Roche GS FLX or ... shotgun and long paired end (LPE) libraries with different jumping ... 40 kb). After sequencing, the resulting reads are assembled into ...
... the most important genes in the human genome is called ... Roger Leng, a researcher in the Faculty of Medicine & ... inactivated in cancerous cells, allowing tumours to grow. , "Successful ... therapies that could potentially improve the prognosis for cancer patients ...
... on improved methods for determining the amounts of flavanol ... by The Hershey Center for Health and Nutrition, was ... other scientific laboratories. Scientists at Planta Analytica ... a large scale. The Hershey scientists and collaborating ...
Cached Biology News:U of Alberta researcher discovers potential cancer therapy target 2Hershey scientists improve methods for analysis of healthful cocoa compounds 2
...
... siSolutions siRNA Custom Synthesis Service ... duplex RNA to knockdown any ... (19 RNA base pairs with ... 27-mers, or other lengths of ...
... Rabbit Antibody to CRF ... with the ovine CRF sequence ... Cells and fibres are found ... of rat brain in colchicine ...
...
Biology Products: