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Multichannel Pipetting: How to Choose the,,,Correct Pipettor

ample, if 50 L is to be dispensed, the range can be as follows: 50-300 L, 30-300 L, 40-350 L, 20-200 L, or 20-300 L, depending on the manufacturer. However, a large volume range should not come at the expense of compromised accuracy and precision, which is often the case (Table 1). Even at low volumes, the piston travel of the mLINE instrument is longer than usual, which makes pipetting more accurate in small volumes. In addition, the minimized dead volume, i.e., the air space between the tip and the piston, further improves accuracy.

Protective tip cone filters and filter ejection
Pipettors are contaminated very easily if no protection is used (filter tips or filter in the pipettor tip cone). Even when working with buffers or aqueous solutions, small amounts of liquid may get inside the tip cone, causing contamination. Special attention should be paid to this problem when working with infectious, radioactive, or DNA samples. An economical and effective way to protect both the pipettor and the sample from contamination is to use a filter in the pipettor tip cone.5 Standard tips can be used with the tip cone filter in many applications, instead of the more expensive filter tips. This feature is offered on Biohit multichannel pipettors (Table 1). Moreover, the mLINE features a thumb-activated filter ejection system with which the filters can be removed safely without the user touching them (Figure 4).6 Very often, the filter is enough to protect the pipettor. However, only autoclaving ensures that the pipettor is virtually free of dangerous pathogens or the agent of concern.

Decontamination and autoclaving
Easy disassembling and autoclaving are regarded as important features for pipettor maintenance. To be able to autoclave the entire pipettor without disassembling the liquid end or having to change the O-rings
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