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Multichannel Pipetting: How to Choose the,,,Correct Pipettor

> The mLINE pipettor is lightweight, and the patented spring mechanism,4 combined with low-friction sealings and grease, ensures that less pipetting force is required (Figure 2). The spring mechanism allows the user to pipet with a constant and very low force, regardless of the volume, which has a direct effect on pipetting accuracy. The smaller the force needed for minute piston movements (small volumes such as 0.5 L), the easier it is to pipet accurately.

Due to the instrument's design, the maximum pipetting force of the mLINE is lower than the minimum pipetting force of pipettors produced by most other manufacturers. mLINE demands four times less work from the user than conventional pipettors. The low pipetting and tip ejection force ensures that precision values easily stay within specification, even after prolonged hours of pipetting. Moreover, because the operating mechanism is thermally insulated from the handle, the warmth of the hand does not affect pipetting results at all, which may be the case with other pipettors, especially after several hours of continuous pipetting.

Figure 1 The mLINE pipettor features very light pipetting and tip ejection force.

Figure 2 The mLINE pipettor features individual piston/ tip cone assemblies, spring-loaded tip cones, low-friction sealings and grease, and full autoclavability.

Tip sealing and tip ejection force
Tip mounting and ejection demands much force in multichannel pipetting, and is directly related to tip sealing and the force with which tips are being mounted to the pipettor. Since the pipettor and the tip are designed as a system to ensure a perfect fit, only tips specified by the pipettor manufacturer should be used. A compromise in tip selection may result in uneven sample pickup, especially wi
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