Navigation Links
Multichannel Pipetting: How to Choose the,,,Correct Pipettor

by Sari Mannonen, Pasi Nieminen, Jukka Kaasinen and Kati Andersin

Multichannel pipettors are widely used in microplate work, including serological testing, molecular biology, immunology assays, and polymerase chain reaction (PCR). The explosion of specific markets such as genome projects and drug discovery has further increased the use of the microplate. Because microplates are currently being used in very labor-intensive applications worldwide, there is a need to increase productivity, maintain accuracy and precision, and reduce physical stress. Each of the approximately ten multichannel pipettor manufacturers emphasizes ergonomics, comfort, ease of use, and safety. Despite this, many users have problems with performance, tip mounting, tip sealing, and tip ejection. The key question is: How does one choose the correct multichannel pipettor? Following are a few criteria that should be considered when choosing a multichannel pipettor.

Ergonomics
All manufacturers claim that their pipettors are ergonomic. However, there exist clear differences in pipetting and tip ejection force as well as in weight, length, and design. For example, the difference between the lightest instrument, the mLINE (Biohit Plc., Finland) (Figure 1), and the heaviest is more than 100 g (137 vs 241 g) (Table 1). Usually, the shorter and lighter the pipettor, the more ergonomic it is to use. However, more important than weight is the balance and fitting of the pipettor to the hand of the user. This is because good stability considerably reduces the strain on the muscles of the hand.1 Most, but not all, multichannel pipettors have a finger support to secure better fitting. Table 1 lists available mechanical multichannel pipettors and their features.

Ease of use
An important feature in multichannel pipettors is the ability to turn the liquid end to an optimal position, for example, when pipetting to microplates (Figure 1). However, many pipettors allow rotation only in one direction, since rotation in the other direction will disassemble the liquid end from the handle. In some models, the connecting nut has to be tightened after adjusting. To avoid accidental disassembling, which leads to a loss of accuracy or even leakage during pipetting, one should ensure that the pipettor chosen allows 360 free rotation without a need for tightening. The mLINE instrument features this ability. Pipettors that are color-coded and have large, clear numbers are easier to read and use. This is also the case with the mLINE unit; in addition, the volume adjustment is stepless, extremely light, and fast to adjust. Most importantly, the volume is locked after adjustment to avoid accidental alteration of the volume during pipetting.2 To avoid this, many pipettors on the market have adjustment systems that are extremely heavy to turn, particularly while wearing gloves.

Pipetting force, accuracy, and precision
There exists much variation among different manufacturers in the specifications for instrument accuracy and precision (Table 1). This regards not only the guaranteed specifications but also the pipetting force, which has an influence on accuracy. The force needed to depress the plunger to the first position varies from 8.7 to 20 N, depending on the manufacturer and the pipettor in use; for the blowout, that number goes up to 45 N (4.5 kg).3 In conventional pipettors, the smaller the volume, the more thumb force that is demanded to operate the plunger. High force, together with small movements, cannot be repeated with the same accuracy after hundreds of pipettings. If one is pipetting more than 1000 samples a day with a conventional mechanical pipettor, it is no surprise that serious fatigue in the muscles of the hand occurs, which can ultimately lead to repetitive strain injuries (RSI).

The mLINE pipettor is lightweight, and the patented spring mechanism,4 combined with low-friction sealings and grease, ensures that less pipetting force is required (Figure 2). The spring mechanism allows the user to pipet with a constant and very low force, regardless of the volume, which has a direct effect on pipetting accuracy. The smaller the force needed for minute piston movements (small volumes such as 0.5 L), the easier it is to pipet accurately.

Due to the instrument's design, the maximum pipetting force of the mLINE is lower than the minimum pipetting force of pipettors produced by most other manufacturers. mLINE demands four times less work from the user than conventional pipettors. The low pipetting and tip ejection force ensures that precision values easily stay within specification, even after prolonged hours of pipetting. Moreover, because the operating mechanism is thermally insulated from the handle, the warmth of the hand does not affect pipetting results at all, which may be the case with other pipettors, especially after several hours of continuous pipetting.

Figure 1 The mLINE pipettor features very light pipetting and tip ejection force.

Figure 2 The mLINE pipettor features individual piston/ tip cone assemblies, spring-loaded tip cones, low-friction sealings and grease, and full autoclavability.

Tip sealing and tip ejection force
Tip mounting and ejection demands much force in multichannel pipetting, and is directly related to tip sealing and the force with which tips are being mounted to the pipettor. Since the pipettor and the tip are designed as a system to ensure a perfect fit, only tips specified by the pipettor manufacturer should be used. A compromise in tip selection may result in uneven sample pickup, especially with 12-channel pipettors.

However, even when the tips of the original manufacturer are being used, traditional multichannel pipettors require a high insertion force, up to 10 kg, because of the large sealing area. This, in turn, leads to massive force needed to eject the tips, especially when using 12-channel pipettors. The tip ejection force with multichannel pipettors can vary up to threefold depending on the number of channels, tip mounting force, manufacturer, and model. Some manufacturers even recommend rocking the pipettor over the tip rack and tightening the tips by hand to ensure secure tip fitting. This is both time-consuming and causes a possible risk of contamination. Without doubt, it results in laborious tip ejection requiring much force. O-rings or elastomer tip cones eliminate that problem, but require maintenance rather often, and wear particles can contaminate samples. The mLINE Optiload system features spring-loaded tip cones (Figures 2 and 3) that allow pipettor tips to be picked up easily with a constant low force. In practice, tips cannot be sealed too tightly to the tip cones, which prevents overinsertion and jamming of the tips. This system ensures that all tips are always sealed on the same height, which is particularly important in microplate pipetting, in which it is necessary that all 12 tips reach the well bottom evenly. Thus, the springs secure optimal, even tip sealing and consistent sample loading, and reduce tip ejection force considerably, up to 65% (Figure 2). Moreover, the tip ejector design has a large, rounded surface, which makes it convenient for users and allows onehanded, low-force tip ejection. Thus, when hundreds of tip ejections are performed daily, optimal tip loading and ejection significantly reduces the risk for RSI.

Volume range
Much variety exists in the volume ranges of different multichannel pipettors. For example, if 50 L is to be dispensed, the range can be as follows: 50-300 L, 30-300 L, 40-350 L, 20-200 L, or 20-300 L, depending on the manufacturer. However, a large volume range should not come at the expense of compromised accuracy and precision, which is often the case (Table 1). Even at low volumes, the piston travel of the mLINE instrument is longer than usual, which makes pipetting more accurate in small volumes. In addition, the minimized dead volume, i.e., the air space between the tip and the piston, further improves accuracy.

Protective tip cone filters and filter ejection
Pipettors are contaminated very easily if no protection is used (filter tips or filter in the pipettor tip cone). Even when working with buffers or aqueous solutions, small amounts of liquid may get inside the tip cone, causing contamination. Special attention should be paid to this problem when working with infectious, radioactive, or DNA samples. An economical and effective way to protect both the pipettor and the sample from contamination is to use a filter in the pipettor tip cone.5 Standard tips can be used with the tip cone filter in many applications, instead of the more expensive filter tips. This feature is offered on Biohit multichannel pipettors (Table 1). Moreover, the mLINE features a thumb-activated filter ejection system with which the filters can be removed safely without the user touching them (Figure 4).6 Very often, the filter is enough to protect the pipettor. However, only autoclaving ensures that the pipettor is virtually free of dangerous pathogens or the agent of concern.

Decontamination and autoclaving
Easy disassembling and autoclaving are regarded as important features for pipettor maintenance. To be able to autoclave the entire pipettor without disassembling the liquid end or having to change the O-rings that many multichannels feature is always an advantage, even if total disassembling is sometimes needed for cleaning. However, there are also convenient and effective decontamination solutions available on the market, which require simple spraying and wiping instead of time-consuming autoclaving.5

When choosing a multichannel pipettor, the user should make sure that it is fully autoclavable; in many pipettors, only the lower part can be autoclaved or the unit is not autoclavable at all. If autoclaving is done frequently, the pipettor, like the mLINE, should be fully autoclavable (numerous cycles) and should not need prior disassembling.

Calibration and in-house service
When purchasing a multichannel pipettor, the user should make sure that it is CE marked, and can easily be calibrated and serviced in-house when necessary. In many multichannel pipettors, service is time-consuming, requiring extra tools and many different parts to be disassembled, cleaned, and reassembled in the correct order. This makes self-service practically impossible, especially if one broken tip cone requires a time-consuming change of the whole liquid end. For the user to be able to service the pipettor quickly and effectively, the multichannel pipettor should have individual piston/tip cone assemblies, and only 1-2 parts to be cleaned, as is the case with the mLINE. The calibration of the instrument has been made to be as user-friendly as possible. The calibration tool, which also acts as a tube cap opener, enables fast and accurate calibration. Most importantly, it allows fine adjustment. Due to a special transmission mechanism, even very small increments in calibration can be done easily and safely.

Figure 3 The Optiload system features springloaded tip cones that allow pipettor tips to be picked up with a constant force. This, in turn, ensures even tip sealing and sample pickup, and considerably reduces tip ejection force compared to conventional pipettors.

Figure 4 With the instrument's filter ejection mechanism, filters can be removed simply by pressing the piston all the way down to the third position.

Conclusion
There are several types of multichannel air displacement pipettors on the market. The best way to find the right tool for each application is to go through the pipetting procedures and find out which pipetting techniques and volumes are needed. However, less force in pipetting and tip ejection means less fatigue and discomfort, which results in improved productivity and user satisfaction. Therefore, one should look for a light, low-force, ergonomic multichannel pipettor that does not compromise accuracy and precision. It is preferable that tips designed for the particular pipettor are used. Before choosing a multichannel pipettor, the user should ask for a trial run to test the instrument. The best decision comes through evaluation of one's own experience.

References

  1. David G, Buckle PA. A questionnaire survey of the ergonomic problems associated with pipettes and their usage with specific reference to work-related upper limb disorders. Appl Ergonom 1997; 28 (4):257-62.
  2. FI patents 86968, EP 0527170, JP 3168296, and U.S. 5,347,878.
  3. Mannonen S, Nieminen P, Kaasinen J, Andersin K. Raising the standard of mechanical pipetting. Int Labmate 2003; 28(5):34-5.
  4. FI patent 109407 and patents pending.
  5. Kolari M, Mannonen S, Takala T, Saris P, Suovaniemi O, Salkinoja-Salonen MS. The effect of filters on aseptic pipetting lifetime of mechanical and electronic pipettors and carryover during pipetting. Lett Appl Microbiol 1999; 29:123-9.
  6. FI patents 108281 and 104885 and patents pending.

Dr. Mannonen is Vice President, Sales and Marketing, Mr. Nieminen and Mr. Kaasinen are Mechanical Designers, and Ms. Andersin is Product Manager, Biohit Plc., Laippatie 1, 00880 Helsinki, Finland; tel.: +358 9 773 861; fax: +358 9 773 86 200; e-mail: sari.mannonen@biohit.com.
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Verifying Multichannel Pipettor Performance with Standard Dispense Solutions in the SpectraMax Plus (MaxLine Application Note #28)
2. Choose the Right Transfection Agent for Your RNAi Experiment
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Multichannel Pipetting How Choose the Correct Pipettor

(Date:7/7/2015)... ... July 07, 2015 , ... bioLytical Laboratories Inc. announced today ... accepted for presentation at the 8th Annual International AIDS Society (IAS) Conference on ... July 22nd from 1:00 pm to 2:00pm Pacific Standard Time at the poster ...
(Date:7/7/2015)... ... 2015 , ... uBiome, the leading microbial genomics company, is offering talented researchers ... full 16S laboratory analysis. The goal is to fast-track a study with the greatest ... their own dashboard, enabling them to view and compare the results of their study, ...
(Date:7/7/2015)... ... July 07, 2015 , ... ... to lower barriers to innovation in life science R&D, has attracted a number ... quarter include another top ten pharmaceutical company joining as a Core member, and ...
(Date:7/6/2015)... 6, 2015  Emdeon Inc. today announced ... agreement to acquire Altegra Health, a national ... combine data aggregation and analytics with unique ... actionable insights and improved management for value-based ... risk adjustment and quality analytics and Emdeon,s ...
Breaking Biology Technology:bioLytical to Present Findings of Early HIV Detection 2bioLytical to Present Findings of Early HIV Detection 3uBiome to Award Grant of up to $100k for Groundbreaking Microbiome Research 2uBiome to Award Grant of up to $100k for Groundbreaking Microbiome Research 3Pistoia Alliance Continues to Attract New Members 2Emdeon to Acquire Altegra Health 2Emdeon to Acquire Altegra Health 3Emdeon to Acquire Altegra Health 4
... devised by researchers from the National Institute of Standards ... flame retardants used in the polyurethane foam of upholstered ... perhaps by as much as 1,130 percent. The ... ,* suggest that significant fire-safety advantages can be gained ...
... Susan G. Komen for the Cure ® Los ... cancer advocacy organization, is proud to announce a partnership with ... advocate, Kelly Price .  Susan G. Komen for the ... and education. The Los Angeles County Affiliate ...
... 3, 2011 HumanTouch, LLC announced that it ... contract by the U.S. General Services Administration. 8(a) ... contract engineered to provide cutting-edge technology solutions from ... designation is designed to promote small business utilization ...
Cached Biology Technology:Novel coatings show great promise as flame retardants in polyurethane foam 2Kelly Price Joins Los Angeles County Affiliate of Susan G. Komen for the Cure in the Fight Against Breast Cancer 2Kelly Price Joins Los Angeles County Affiliate of Susan G. Komen for the Cure in the Fight Against Breast Cancer 3Kelly Price Joins Los Angeles County Affiliate of Susan G. Komen for the Cure in the Fight Against Breast Cancer 4HumanTouch Awarded 8(a) STARS II GWAC Contract 2
(Date:6/18/2015)... NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" or the "Company") ... market announces that its Wocket® smart wallet will be the ... scheduled to air on WABC Radio (am770) in ... The broadcast air- time for the extensive one ... Inc.,s CEO Gino Pereira will be discussing its ...
(Date:6/17/2015)... June 17, 2015  Crossmatch™, a leading provider ... its U.are.U ® 4500 fingerprint readers have ... Eateries, Inc., a San Jose, CA ... increase security, improve accountability and reduce fraud at ... non-repudiable identity confirmation for employee sign in and ...
(Date:6/16/2015)... 16, 2015 Fingerprint Cards (FPC) raises ... it will amount to at least 340 MSEK to that ... rapid increase in market growth and orders received the revenue ... that revenue for 2015 will exceed 1,500 MSEK to an ... 2,200 MSEK. Due to receipt of orders of ...
Breaking Biology News(10 mins):NXT-ID, Inc.'s CEO Gino Pereira Talks About the Wocket Smart Wallet on WABC Radio, New York City, June 20th 2NXT-ID, Inc.'s CEO Gino Pereira Talks About the Wocket Smart Wallet on WABC Radio, New York City, June 20th 3Togo's Eateries Improve Security and Accountability with Crossmatch Fingerprint Readers 2Togo's Eateries Improve Security and Accountability with Crossmatch Fingerprint Readers 3Fingerprint Cards Raises the Revenue Guidance for Q2 2015 and for the Full Year 2015 2Fingerprint Cards Raises the Revenue Guidance for Q2 2015 and for the Full Year 2015 3
... factors that signal whether an athlete will experience concussive ... risk factors for prolonged concussion symptoms by examining a ... concussion studies were limited in scope, focusing only on ... to male and female athletes from a number of ...
... intensive care units have difficulty finding private, quiet places ... new study from the Frances Payne Bolton School of ... of privacyif it causes mothers to miss feedings or ... greater health risk for low-weight premature newborns, because milk ...
... Scientists at Singapore Eye Research Institute and A*STAR,s Genome ... central corneal thickness that may cause potentially blinding eye ... the progressive thinning of the cornea, which may eventually ... authors jointly led a multi-centre study involving 55 hospitals ...
Cached Biology News:Privacy a problem for mothers of newborns in neonatal intensive care units, CWRU study finds 2First in the world - Singapore scientists discover genes responsible for cornea blindness 2First in the world - Singapore scientists discover genes responsible for cornea blindness 3
... This CLS number is ... to easily match Cornings product ... yet, please order under the ... contact customer service for assistance. ...
... GenePix Professional 4200A can be upgraded to ... automatically loads, scans, analyzes, and saves results ... you by email when the batch is ... even for warped slides. GenePix Pro gives ...
... of your microarray data using Asuragens bioinformatics ... from consultation to extensive data analysis using ... our expertise in combination with the Gene ... microarray experiments., ,Normalization and basic statistical analysis ...
... the value of your microarray data ... the support you need from consultation ... most up to date methods. Utilize ... Gene Expression Profiling Service or from ...
Biology Products: