Navigation Links
Mouse Tail Genomic DNA Isolation Protocol(1)

Note: Genomic DNA is fragile. High molecular weight DNA is sheared easily by mechanical forces. Use suitable large-bore pipet tips or equipment when pipetting genomic DNA. Do not vortex solutions containing genomic DNA.
  1. Place a 1 cm tail sample into a 1.5 ml microcentrifuge tube; this may be stored at 20C. To minimize possible cross-contamination, do not mince the sample. Add 700 l Tail Buffer (50 mM Tris-HCl, pH 8.0, 100 mM EDTA, 100 mM NaCl, 1% SDS) to the sample.
  2. Add 35 l 10 mg/ml Proteinase K to the sample and mix briefly.
  3. Incubate at 5560C overnight with mixing. This step should result in the complete solubilization of the tail fragment. In the case of incomplete digestion, more Proteinase K can be added and the samples incubated for several more hours.
  4. Add 20 l 10 mg/ml RNase A (DNase-free) to the sample. Mix briefly and incubate at 37C for 12 hours.
  5. Transfer entire solution to a pre-spun (1500 x g for 12 minutes) PLG 2 ml Heavy tube.
  6. Add 0.5 ml Phenol-Chloroform-Isoamyl Alcohol (PCI, 25:24:1) to the sample in the PLG 2 ml tube and mix well by repeated inversion. Do not vortex.
  7. Centrifuge at full speed (12,000 x g or greater) for 5 minutes in a microcentrifuge, then carefully transfer the resultant aqueous phase to a fresh pre-spun PLG 2 ml Heavy tube.
  8. Add 0.5 ml Chloroform-Isoamyl Alcohol (CI, 24:1) to the sample in the PLG 2 ml tube and mix well by repeated inversion. Do not vortex.
  9. Centrifuge at full speed (12,000 x g or greater) for 5 minutes in a microcentrifuge, the
    '"/>


Source:


Page: All 1 2

Related biology technology :

1. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
2. Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
3. Mouse Tail Genomic DNA Isolation Protocol(1)
4. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
5. Cenix-Designed siRNAs for 95% of Human and Mouse Genomes
6. RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control
7. Simultaneous Detection of 8 Cytokines in Mouse or Human Sera
8. A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays
9. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
10. New Protocols for Isolating High- Molecular-Weight Genomic DNA
11. Easily Amplify Genomic DNA with Long-Distance PCR
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Mouse Tail Genomic DNA Isolation Protocol

(Date:12/19/2014)... (PRWEB) December 19, 2014 BioPlus ... specialty pharmacies, announces the promotion of Nick Maroulis, Pharm.D. ... Specialty Pharmacy Services. , In this position, Dr. ... while also managing the directors of our multi-site pharmacies ... BioPlus since 1997 and during that time he has ...
(Date:12/19/2014)... PLEASANTON, Calif. , Dec. 19, 2014  Roche ... acquisition of Bina Technologies, Inc. (Bina), a privately ... California , USA. Bina provides a big ... next generation sequencing (NGS) data. Bina,s proprietary on-market ... translational and academic researchers to perform fast and ...
(Date:12/19/2014)... -- Decision Resources Group finds that the Latin American ... compound annual growth rate through 2023. This is primarily ... increasing adoption of dental implants. Growing aesthetic consciousness in ... demand for dental biomaterials because they can improve the ... Other key findings from Decision Resources Group,s coverage ...
(Date:12/19/2014)... Springs, FL (PRWEB) December 18, 2014 ... of the nation’s leading specialty pharmacies, has an ... serve as clinical pharmacy consultants for prescriber offices ... therapy expertise. , “We have a variety ... therapies. For example, those specializing in hepatitis C ...
Breaking Biology Technology:BioPlus Specialty Pharmacy Promotes Dr. Nick Maroulis to Vice President of Specialty Pharmacy Services 2Roche acquires Bina Technologies and enters the genomic informatics market 2Roche acquires Bina Technologies and enters the genomic informatics market 3Latin American Dental Biomaterial Market Will Expand at a 12 Percent Annual Growth Rate Through 2023 2Latin American Dental Biomaterial Market Will Expand at a 12 Percent Annual Growth Rate Through 2023 3BioPlus Specialty Pharmacy Expands ‘Regional Pharmaceutical Care Liaison’ Team 2BioPlus Specialty Pharmacy Expands ‘Regional Pharmaceutical Care Liaison’ Team 3
... First Capability To Rapidly Identify Five Candida ... Positive Blood Cultures In One Test, WOBURN, ... FDA 510(k) clearance of the Yeast Traffic Light ... positive blood cultures. The,Yeast Traffic Light(TM) is the ...
... 23 Affitech AS, the Norwegian human,antibody ... accomplished the,second milestone in its research and ... recombinant antibodies against an unnamed oncology,target. Affitech ... Antibody,Screening) system that included its proprietary antibody ...
... 22, 2008 -- Chemists at Rice University have discovered ... tiny, wand-like nanoparticles that are being studied in dozens ... disease and improving electronic viewscreens. , "The content of ... is only about 20 percent," said lead researcher Eugene ...
Cached Biology Technology:AdvanDx Receives FDA 510(k) Clearance for Yeast Traffic Light PNA FISH(TM) 2AdvanDx Receives FDA 510(k) Clearance for Yeast Traffic Light PNA FISH(TM) 3Affitech Announces Second Milestone in Roche Antibody Discovery Collaboration 2Purifying nanorods: Big success with tiny cleanup 2
(Date:12/5/2014)... VIEW, Calif. , Dec. 4, 2014 /PRNewswire/ ... safety has boosted investments in new testing and ... has also given rise to a range of ... by the requirements of Generation Y, which is ... technologies. As a result, product development strategies of ...
(Date:11/21/2014)... , November 18, 2014 According ... Market by Systems (Video, RFID, Access Control, Intrusion Detection, ... Hotels, Banks, Government), Component Service Geography - Global Forecasts ... Market is projected to be around $25 Billion in ... 2020, growing at a CAGR of 8.69%. ...
(Date:11/18/2014)... , Nov. 18, 2014  The Secure ... Industry Association today jointly announce the formation of ... release of its Identity and Biometric Entry ... The Framework received official support from BORDERPOL, the ... to improve and provide expertise regarding border security, ...
Breaking Biology News(10 mins):Emerging Technologies Enable Smarter Monitoring of Assets, Push Industrial Testing, Inspection and Monitoring Market 2Emerging Technologies Enable Smarter Monitoring of Assets, Push Industrial Testing, Inspection and Monitoring Market 3Emerging Technologies Enable Smarter Monitoring of Assets, Push Industrial Testing, Inspection and Monitoring Market 4Industrial Security Systems Market Worth $38 Billion by 2020 2Industrial Security Systems Market Worth $38 Billion by 2020 3Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 2Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 3
... as butterflies are effective against predators because they ... eyes of the predators own enemies, according to ... Zoologists based at the University of Cambridge challenge ... effective against predators. Many animals possess ...
... in Spanish . , ANN ARBOR, Mich.---University ... Institute on Aging have produced the largest and most ... trove offering new insights into early migrations out of ... build ever-larger telescopes to peer deeper into space, population ...
... but beaver can help mitigate the effects of drought, ... accommodate industrial, urban and agricultural demands should be avoided, ... Removal of beaver should be considered an environmental disturbance ... extraction, said researcher Glynnis Hood, lead author on the ...
Cached Biology News:Zoologists challenge longstanding theory that 'eyespots' mimic the eyes of predators' enemies 2U-M researchers release most detailed global study of genetic variation 2U-M researchers release most detailed global study of genetic variation 3Beavers can help ease drought 2
Carrier Serum...
Our original highly-purified, robust Taq Polymerase which delivers consistently high yields and minimal background...
... is a derivative of Taq DNA polymerase. ... aquaticus DNA polymerase. Expressed from a gene ... Met236, bypassing the 5'-3' exonuclease domain of ... a highly active and even more heat-stable ...
... Dinitro-5,5'-dithiodibenzoic acid , DTNB , Ellman's reagent ... , *CAS-No.: 69-78-3 *Storage: RT, ... a reactive grouping not present in the substrate, ... which will react directly with the product to ...
Biology Products: