Navigation Links
Mouse Tail Genomic DNA Isolation Protocol(1)

n carefully transfer resultant aqueous phase to a fresh microcentrifuge tube.
  • Fill sample-containing tube with 100% Isopropanol and mix thoroughly by repeated inversion. Do not vortex. A visible DNA precipitate should form. Proceed immediately to step 11.
  • Recover DNA precipitate by touching it to a heat-sealed glass micropipette tip or by lifting the DNA with a yellow pipette tip and partial suction from a pipettor. Transfer the DNA to a 1.5 ml microcentrifuge tube containing 70% Ethanol. If DNA is not stringy, pellet by a brief, low speed centrifugation.
  • Wash DNA with the 70% Ethanol, then wash twice with 95% Ethanol.
  • Allow DNA to partially dry and then either transfer the DNA to a microcentrifuge tube containing 400 l TE (10 mM Tris-Cl, 1 mM EDTA, pH 8.0) or add 400 l TE to the DNA in the tube. Do not vortex or re-pipet to resuspend DNA.
  • Resolubilize the DNA overnight (e.g. by rotation at 3060 rpm). Resolubilization may be facilitated by heating the sample at 50C.
  • References
    1. Herrmann, B.G. and Frischauf, A.M. (1987) Isolation of genomic DNA. Methods Enzymol. 152:180-183.
    2. Laws, G.M. and S.P. Adams. (1996) Measurement of 8-OHdG in DNA by HPLC/ECD: The importance of DNA purity. BioTechniques 20:36-38.
    3. Murphy, N.R. and Hellwig, R. J. (1996) Improved nucleic acid organic extraction through use of a unique gel barrier matrix. BioTechniques 21:934-939.
    4. Fil Randazzo, personal communication.

    '"/>

    Source:


    Page: All 1 2

    Related biology technology :

    1. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
    2. Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
    3. Mouse Tail Genomic DNA Isolation Protocol(1)
    4. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
    5. Cenix-Designed siRNAs for 95% of Human and Mouse Genomes
    6. RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control
    7. Simultaneous Detection of 8 Cytokines in Mouse or Human Sera
    8. A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays
    9. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
    10. New Protocols for Isolating High- Molecular-Weight Genomic DNA
    11. Easily Amplify Genomic DNA with Long-Distance PCR
    Post Your Comments:
    *Name:
    *Comment:
    *Email:
    TAG: Mouse Tail Genomic DNA Isolation Protocol

    (Date:5/26/2015)... ANGELES , May 26, 2015 Pharmaco-Kinesis ... Board (NOCAB) recently convened at Shutters on the Beach ... review the development status of the Company,s innovative Metronomic ... designed to locally deliver chemotherapy over time to a ... dose delivery capacity and biofeedback system in real-time.  ...
    (Date:5/26/2015)... , May 26, 2015  Asterias Biotherapeutics, Inc. ... emerging field of regenerative medicine, today announced that all ... 2014 were exercised prior to their expiry on June ... of $11.7 million. "We thank our shareholders ... President and CEO of Asterias. "This exercise of warrants ...
    (Date:5/26/2015)... and NEW YORK , May 26, 2015 ... NASDAQ: NVGN ), announced today that it will present at ... 2015 in New York City at the ... Graham Kelly , Novogen Chairman and CEO, is scheduled to ... live webcast. To access the webcast, go to http://wsw.com/webcast/marcum3/nvgn . ...
    (Date:5/25/2015)... 2015 Where is the universe expanding ... "The honest answer is that nobody is entirely sure." ... throughout the month of June, starting with the ULI ... future-proofing to top tech trends. And one of the ... the universe is expanding to. According to Einstein’s theory ...
    Breaking Biology Technology:Pharmaco-Kinesis Corporation Convenes Third Neuro-Oncology Clinical Advisory Board Meeting 2Pharmaco-Kinesis Corporation Convenes Third Neuro-Oncology Clinical Advisory Board Meeting 3Pharmaco-Kinesis Corporation Convenes Third Neuro-Oncology Clinical Advisory Board Meeting 4Pharmaco-Kinesis Corporation Convenes Third Neuro-Oncology Clinical Advisory Board Meeting 5Asterias Biotherapeutics Receives $11.7 Million in Proceeds from Exercise of Warrants 2Asterias Biotherapeutics Receives $11.7 Million in Proceeds from Exercise of Warrants 3Novogen to Present at the 2015 Marcum Microcap Conference 2Novogen to Present at the 2015 Marcum Microcap Conference 3Novogen to Present at the 2015 Marcum Microcap Conference 4Futurist Jack Uldrich Says Embracing Our Ignorance is the Key to Unlearning 2Futurist Jack Uldrich Says Embracing Our Ignorance is the Key to Unlearning 3
    ... NEW YORK, Jan. 19, 2012   Reportlinker.com ... is available in its catalogue: , ... in the United States ... the U.S. market for collagen peptides. The ...
    ...  Synthetic Genomics Inc. (SGI), a privately held company commercializing ... of global challenges, today announced they have hired George ... is a proven leader with experience in developing sophisticated ... and nutritional products for humans and animals. At SGI ...
    ... growth factors derived from a woman,s own blood, physician ... a simple, office-based procedure that appears to markedly improve ... results of this procedure, which involves the injection of ... the upper wall of the vagina and the clitoris, ...
    Cached Biology Technology:Strategic Analysis of the Collagen Peptide Market in the United States 2Synthetic Genomics Inc. Hires Industry Leader George E. Stagnitti to Head Food and Nutritional Products Development Program 2"O-Shot™" May Improve Female Sexual Response 2"O-Shot™" May Improve Female Sexual Response 3
    (Date:4/20/2015)... , April 20, 2015 Huntington Memorial ... Valley to implant a new miniaturized, wireless monitoring sensor ... is the first and only FDA-approved heart failure monitoring ... admissions when used by physicians to manage heart failure. ... that is implanted in the pulmonary artery (PA) during ...
    (Date:4/17/2015)... 2015 Increasing adoption ... advancement to drive biometric systems market in ... According to a recently released TechSci ... ", biometric systems market in Saudi ... at CAGR of over 22% through 2020. The ...
    (Date:4/14/2015)... OXFORD, Conn. , April 14, 2015 ... "Company"), a biometric authentication company focused on the growing ... has been nominated for the 2015 New York Design ... The Design100, New York City Design Awards are ... are based on over 2,500 ratings from the marketplace, ...
    Breaking Biology News(10 mins):Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 2Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 3Saudi Arabia Biometric Systems Market to Grow at 22% Through 2020, Says TechSci Research 2Saudi Arabia Biometric Systems Market to Grow at 22% Through 2020, Says TechSci Research 3NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 2NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 3
    ... PHILADELPHIA - Flax has been part of human history for ... and animals, and even making paint. Now, researchers from ... have discovered that it might have a new use for ... harmful effects of radiation. In a study just published ...
    ... LAKE CITY, Aug. 9, 2011 Life is tough for ... plants for food, and those plants produce poison to deter ... study shows how certain woodrats put themselves on a diet ... eat smaller meals, increase time between meals and drink more ...
    ... 8 Researchers at the University of Pittsburgh School ... explain how an enzyme elevated in asthma patients can ... characteristic of the lung condition. Their findings, reported online ... of Sciences , reveal unique interactions between biological molecules ...
    Cached Biology News:Penn study shows an ancient crop effective in protecting against a 21st century hazard 2Penn study shows an ancient crop effective in protecting against a 21st century hazard 3Rats control appetite for poison 2Rats control appetite for poison 3Rats control appetite for poison 4Pitt team finds molecular pathway that leads to inflammation in asthma 2
    ... The FastPlax Titer Kit is designed for ... instead of 46 days as in other methods. ... the appearance of plaques, but the FastPlax Kit ... of AcNPV gp64 glycoprotein on the cell surface ...
    ...
    ... has been designed for recovery of DNA from TAE ... Ultra fast protocol: DNA is ready to ... easy to resuspend Bead uniformity prevents shearing of ... for PCR) DNA is eluted into sterile water ...
    ... is ideal for the ... (1D electrophoresis, 2D electrophoresis, ... Pharmacokinetics & Toxicology (whole ... chromatography); Physical and Material ...
    Biology Products: