Navigation Links
Mouse Tail Genomic DNA Isolation Protocol(1)

Note: Genomic DNA is fragile. High molecular weight DNA is sheared easily by mechanical forces. Use suitable large-bore pipet tips or equipment when pipetting genomic DNA. Do not vortex solutions containing genomic DNA.
  1. Place a 1 cm tail sample into a 1.5 ml microcentrifuge tube; this may be stored at 20C. To minimize possible cross-contamination, do not mince the sample. Add 700 l Tail Buffer (50 mM Tris-HCl, pH 8.0, 100 mM EDTA, 100 mM NaCl, 1% SDS) to the sample.
  2. Add 35 l 10 mg/ml Proteinase K to the sample and mix briefly.
  3. Incubate at 5560C overnight with mixing. This step should result in the complete solubilization of the tail fragment. In the case of incomplete digestion, more Proteinase K can be added and the samples incubated for several more hours.
  4. Add 20 l 10 mg/ml RNase A (DNase-free) to the sample. Mix briefly and incubate at 37C for 12 hours.
  5. Transfer entire solution to a pre-spun (1500 x g for 12 minutes) PLG 2 ml Heavy tube.
  6. Add 0.5 ml Phenol-Chloroform-Isoamyl Alcohol (PCI, 25:24:1) to the sample in the PLG 2 ml tube and mix well by repeated inversion. Do not vortex.
  7. Centrifuge at full speed (12,000 x g or greater) for 5 minutes in a microcentrifuge, then carefully transfer the resultant aqueous phase to a fresh pre-spun PLG 2 ml Heavy tube.
  8. Add 0.5 ml Chloroform-Isoamyl Alcohol (CI, 24:1) to the sample in the PLG 2 ml tube and mix well by repeated inversion. Do not vortex.
  9. Centrifuge at full speed (12,000 x g or greater) for 5 minutes in a microcentrifuge, the
    '"/>


Source:


Page: All 1 2

Related biology technology :

1. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
2. Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
3. Mouse Tail Genomic DNA Isolation Protocol(1)
4. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
5. Cenix-Designed siRNAs for 95% of Human and Mouse Genomes
6. RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control
7. Simultaneous Detection of 8 Cytokines in Mouse or Human Sera
8. A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays
9. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
10. New Protocols for Isolating High- Molecular-Weight Genomic DNA
11. Easily Amplify Genomic DNA with Long-Distance PCR
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Mouse Tail Genomic DNA Isolation Protocol

(Date:1/15/2014)... 15, 2014 2013 was a banner ... State Technologies®. They saw continued independent research led by ... were awarded a $1 million grant from the Susanne ... and Behavior” a peer reviewed journal, Amy Grant highlighted ...
(Date:1/14/2014)... Date: Friday, April 11, 2014 , Time: 6 p.m. – ... Warrington, Pa. , Details: The Hepatitis B Foundation, ... cure for hepatitis B and improving the quality of life ... on Friday, April 11 at Warrington Country Club in Warrington, ...
(Date:1/14/2014)... pet owners drew up their lists of New Year,s resolutions for ... of my furry companion." Nowadays there are pet salons, dog and ... take the little canine or feline darlings along wherever mommy or ... pricey toys at the pet store. But anyone who has ever ...
(Date:1/14/2014)... iLab Solutions, the leader in core facility ... of Product Strategy. In this role, Michelle will work with ... guide in the development of iLab products. Her main goal ... benefit to the scientific community by offering the most advanced, ...
Breaking Biology Technology:Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 2Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 3Hepatitis B Foundation to Host Annual Crystal Ball Gala 2New Year's Resolution: Give Pets the Gift of Top-Notch Health Care 2iLab Solutions Announces Michelle Detwiler as the New Director of Product Strategy 2
... Sept. 8 /PRNewswire-FirstCall/,-- Concuity, a healthcare division ... a leading,provider of contract management and revenue ... today announced that Valley Baptist Health,System has ... more,accurately calculate payments from managed care and ...
... (Nasdaq: CTICD ; and MTA: CTIC) announced ... Listing Qualifications Staff of The NASDAQ Stock,Market LLC ... with the,$50,000,000 minimum market value of listed securities ... the Company does not,comply with NASDAQ Marketplace Rule ...
... Just as test pilots push planes to explore ... Standards and Technology (NIST) are probing the newest ... the nanoscale. Better nanoscale measurements are critical for ... and nanomanufacturing industries. , This new microscope technology ...
Cached Biology Technology:Valley Baptist Health System Selects Concuity's ClearContracts to More Accurately Calculate Payments from Managed Care and Government Payers 2Valley Baptist Health System Selects Concuity's ClearContracts to More Accurately Calculate Payments from Managed Care and Government Payers 3Valley Baptist Health System Selects Concuity's ClearContracts to More Accurately Calculate Payments from Managed Care and Government Payers 4CTI Receives NASDAQ Letter 2CTI Receives NASDAQ Letter 3NIST studies how new helium ion microscope measures up 2
(Date:7/9/2014)... Sexual selection refers to species, selection for traits that ... of natural selection enhances opportunities to mate, the tail ... at the University of California, Riverside have now found ... a placenta are linked. Describing the life histories ... family Poeciliidae, the researchers found that species with placentas ...
(Date:7/9/2014)... (TCGA) Research Network have identified novel mutations in ... most common subtype of lung cancer. Knowledge of ... possible therapeutic targets for this disease and potentially ... mutations because many potent cancer drugs that target ... funded and managed by the National Cancer Institute ...
(Date:7/9/2014)... With grilling season upon us, many backyard cooks are ... fatty hot dogs. But low fat can sometimes mean ... addressing the texture problem in low-fat wieners that are ... study was published in ACS, Journal of Agricultural ... colleagues note that hot-dog consumers have come to expect ...
Breaking Biology News(10 mins):Biologists link sexual selection and placenta formation 2Biologists link sexual selection and placenta formation 3Biologists link sexual selection and placenta formation 4Study identifies novel genomic changes in the most common type of lung cancer 2Study identifies novel genomic changes in the most common type of lung cancer 3
... those hoping to start or extend a family should ... Current clinical guidelines already recommend that women who are ... based on the knowledge that cholesterol is essential for ... the risk of congenital anomalies in children of pregnant ...
... pollution are decreasing the growth of trees in the northern ... in Global Change Biology . Tree growth, measured ... and this is set to increase to a 17% reduction ... four times greater now than prior to the Industrial Revolution ...
... momentum of,September,s impressive showing at the ePassports EAC Conformity ... advances its role as the,world,s ePassport leader by being ... advanced second-generation ePassports based on the,Extended Access Control (EAC) ... ePassports represent the beginning of an evolution that,will improve ...
Cached Biology News:Statin warning for pregnant women 2Surface-level ozone pollution set to reduce tree growth 10 percent by 2100 2The Finnish Population Register Centre to Deploy Entrust EAC ePassport CA Solution 2The Finnish Population Register Centre to Deploy Entrust EAC ePassport CA Solution 3The Finnish Population Register Centre to Deploy Entrust EAC ePassport CA Solution 4
... SYBR Green II RNA gel stain ... has bright fluorescence when bound to RNA and ... use with either formaldehyde/agarose or polyacrylamide gels using ... stains 50 minigels. A 1 mL unit size ...
Sirtuin 7 (SIRT7) Rabbit anti-Human Polyclonal Antibody Immunogen: Synthetic peptide - KLH conjugated Family: Other Recommended Storage: Long term: -20C; Short term: +4C...
... RSH-4000 is the only microplate sealer with ... long walkaway times with a variety of ... Fully automated with integrated stacker and 240-plate ... EX for use in small workcells , ...
... Reagents for the differentiation of mouse embryonic ... bodies exist in the midbrain, where they ... the ventral tegmental area, and the retrorubral ... to extensive forebrain dopamine innervation ( e.g.projections ...
Biology Products: