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More siRNA Vectors for RNA Interference

beled secondary antibody; Blue: DAPI stained nuclei.


The pSilencer 2.0-U6 and 3.0-H1 vectors each include four components: (1) a linearized and purified vector ready for ligation; (2) a GAPDH-specific siRNA insert that can be used as a positive control; (3) a circular, negative control pSilencer vector that expresses an siRNA with limited homology to any known sequences in the human, mouse, and rat genomes; and (4) 1X DNA Annealing Solution to prepare DNA oligonucleotides for ligation. The pSilencer 1.0-U6 vector is available in both linear and circular formats. The linear pSilencer 1.0-U6 vector is supplied with linearized and purified vector ready for ligation, and a small amount of circular vector containing a GAPDH-specific siRNA insert for use as a positive control.


REFERENCES

1. Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, and Tuschl T. (2001a). Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411, 494-498.

2. Tuschl T. (2002) Expanding small RNA interference. Nature Biotechnology 20, 446-448.

3. Elbashir, SM, Lendeckel, W, and Tuschl, T. (2001b) RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes and Dev. 15, 188-200.

4. Sui G, Soohoo C, Affar EB, Gay F, Shi Y, Forrester WC, and Shi Y. (2002) A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc
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