The pSilencer 2.0-U6 and 3.0-H1 vectors each include four components: (1) a linearized and purified vector ready for ligation; (2) a GAPDH-specific siRNA insert that can be used as a positive control; (3) a circular, negative control pSilencer vector that expresses an siRNA with limited homology to any known sequences in the human, mouse, and rat genomes; and (4) 1X DNA Annealing Solution to prepare DNA oligonucleotides for ligation. The pSilencer 1.0-U6 vector is available in both linear and circular formats. The linear pSilencer 1.0-U6 vector is supplied with linearized and purified vector ready for ligation, and a small amount of circular vector containing a GAPDH-specific siRNA insert for use as a positive control.
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