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Monitoring Receptor-Mediated Changes in [Ca2+]i Using Single-Wavelength and Dual-Wavelength Indicators on the FlexStation Workstation

satile tool for pharmacological and drug screening laboratories and has proven to be suitable for kinetic, cell-based fluorometric assays, such as the measurement of intracellular calcium. Additionally, FlexStation benefits from the ability to use ratiometric dyes to help improve data fidelity, although the Molecular Devices homogeneous assay kits have been shown to achieve the same goal with fewer steps in the assay.


ACKNOWLEDGEMENTS
Many thanks to Dr. Frank Hafner for his valuable opinion and consulting contribution in this study.


REFERENCES
1. Berridge, M.J. (1997). Elementary and global aspects of calcium signalling. J. Physiol., 499, 291 - 306.

2. Haughland, R.P. (2000). Molecular Probes Handbook of Fluorescent Probes and Research Chemicals. Chapter 20 - Indicators for Ca2+, Mg2+, Zn2+ and Other Metal Ions.

3. Endogenous GPCR List by Agi Schonbrunn, Ph.D. Department of Integrative Biology, Pharmacology and Physiology, University of Texas Health Science Center, Houston, Texas. (http://www.tumor-gene.org/GPCR/gpcr.html)

4. Strassheim, D. and Williams, C.L. (2000). P2Y2 Purinergic and M3 Muscarinic Acetylcholine Receptors Activate Different Phospholipase C- Isoforms That Are Uniquely Susceptible to Protein Kinase C-dependent Phosphorylation and Inactivation. J. Biol. Chem., 275, 39767-39772.

5. Conant, A.R. et al (1998). Characterization of the P2 receptors on the human umbilical vein endothelial cell line ECV304. Br. J. Pharmacol., 125, 357-364.

6. Communi, D., Robaye, B. & Boeynaems, J.M. (1999). Pharmacological characterization of the human P2Y11 receptor. Br. J. Pharmacol., 128, 1199-1206.

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