Navigation Links
Monitoring Receptor-Mediated Changes in [Ca2+]i Using Single-Wavelength and Dual-Wavelength Indicators on the FlexStation Workstation

gative control. The term |c+ c- | denotes the absolute value of the difference between the mean of the positive control and the mean of the negative control. A Z-factor > 0.5 indicates a large separation band between the negative and positive controls and an excellent assay.


RESULTS

ATP elicited a concentration-dependent increase in [Ca2+]i in CHO cells (Figure 1) with similar EC50 values for cells loaded with Fluo-3, Fura-2 and FlexStation Calcium Assay Kit, respectively (Table 2).

One-way Analysis of Variance (ANOVA) indicated that the EC50 estimates were not significantly different from one another (P value = 0.62).


These results indicate that there is negligible difference in the ATP EC50 values from cells loaded with the three fluorescent reagents; however, there was a significant difference between the peak fluorescent intensities of the two single-wavelength indicators. With Fluo-3 there was an increase in signal of ~3,200 RFU above basal (1.8 fold increase) whilst with the FlexStation Calcium Assay Kit the increase in signal was 32,000 RFU (5.6 fold increase, see Table 1). Peak response tended to be more variable with Fluo-3 (see Figure 1). With Fura-2 there was a 4.8 fold increase in signal above basal.

The screening coefficient window (Z-factor), which reflects the dynamic range of the signal and the data variation for the assay9 was calculated using the buffer addition (negative control) and the 30 M ATP addition data (positive control). Z-factors obtained with Fura-2 and the FlexStation Calcium Assay Kit were 0.60 and 0.68 respectively (Table 1). This shows a large separation band between the negative and positive controls and a reproducible, high quality assay. The Zfactor obtained for the Fluo-3 data was 0.28, representing a useable assay.
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Versatile Reporter Vectors for Monitoring Viral Transduction
2. Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer
3. Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System
4. Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis
5. Direct Monitoring of Solid-Phase Peptide Synthesis by MALDI-TOF MS
6. Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse
7. New statistical algorithms for Monitoring Gene Expression on GeneChip® Probe Arrays
8. Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon Allelic Discrimination Kits
9. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
10. Oil-Free PCR Using the Hot Top Assembly
11. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
Post Your Comments:
(Date:4/17/2015)... BellBrook Labs announced today that ... the powerful Orthogonal Pooled Screening (OPS) Platform developed ... the Lankenau Institute for Medical Research (LCGC) in ... improve successful drug discovery outcomes using far more ... libraries available nowhere else, coupled to a state-of-the-art ...
(Date:4/16/2015)... (PRWEB) April 16, 2015 ... data solutions driven by Semantic Web technology, and ... specialist information and services for the academic and ... that its joint solution ‘Smart Data Lake for ... finalist for the 2015 Bio-IT ‘Best of Show’ ...
(Date:4/16/2015)... Proove Biosciences , Inc. is pleased ... for the 2015 BIO International Convention. Hosted by ... Convention will take place June 15-18 at the Pennsylvania ... BIO contest, industry leaders will be voting for companies ... The community voting period began on April 14, 2015 ...
(Date:4/16/2015)... (PRWEB) April 16, 2015 According ... overexertion involving an outside source was ranked first ... includes injuries related to lifting, pushing, pulling, holding, ... in direct costs. The David Round Company takes ... committed to working hard to develop and design ...
Breaking Biology Technology:BellBrook Labs and Lankenau Institute Partner to Accelerate Drug Discovery using Orthogonal Pooled Screening 2Cambridge Semantics and Informa Named Bio-IT Award Finalist for Smart Data Lake Solution for Clinical Trial and Competitive Intelligence 2Cambridge Semantics and Informa Named Bio-IT Award Finalist for Smart Data Lake Solution for Clinical Trial and Competitive Intelligence 3Proove Bisociences to Participate in the Buzz of BIO at the 2015 BIO International Convention 2Proove Bisociences to Participate in the Buzz of BIO at the 2015 BIO International Convention 3The David Round Company Helps Companies Meet OSHA Standards by Combining Safety, Ergonomics, and Productivity 2
... is known for its innovation and ability to take ... changing the dynamics and places like South Korea, China ... too. India, for example, is gaining ground in software ... that we hear so much about. The advantages the ...
... an idea of the intricacy possible in machining and tool ... EDM, you can tunnel into the thin side of a ... and leave the top and lower surfaces of that same ... used by Steve Plendl, the lead EDM instructor at ...
... exploring new technologies that will result in a more ... Systems based in Menasha, Wisconsin is strategically investing ... of care it offers by focusing on those initiatives ... ,Affinity has been able to prioritize such investments as ...
Cached Biology Technology:Keeping Innovation Vibrant in the U.S. 2Powerful, Intricate Technology Taught in CVTCs New Tool and Die Program 2Powerful, Intricate Technology Taught in CVTCs New Tool and Die Program 3Affinity Health Streamlines IT Governance 2
(Date:4/8/2015)...  Infinisource, a leading provider of SaaS-based Human Capital ... leading supplier of biometric identification and security solutions, ... advanced biometrically-enabled time clock, the companies announced today. ... setting a bold, new standard for the collection ... and mid-size employer. When connected to the iSolved ...
(Date:4/2/2015)... According to preliminary estimates Fingerprint ... higher than 125 MSEK. This exceeds the guidance provided ... revenues for Q1 2015 would be in line with ... 2014. The operating result for the first quarter is ... report will as previously communicated be published on May ...
(Date:3/31/2015)... March 31, 2015  Elephant Talk Communications Corp. (NYSE ... provider of Software Defined Network Architecture (ET Software DNA® ... revenue of approximately $20.4 million for the year ended ... million for the year ended December 31, 2013. ... Company had completed its multi-year transition away from its ...
Breaking Biology News(10 mins):iSolved Time Clock Offers Unmatched Accuracy due to Morpho Biometrics 2iSolved Time Clock Offers Unmatched Accuracy due to Morpho Biometrics 3Fingerprint Cards Revises Revenues for Q1 2015 2Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 2Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 3Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 4Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 5Elephant Talk Reports 2014 Fourth Quarter and Year End Financial Results 6
... will host its 2014 Biodefense and Emerging Diseases Research ... Hotel in Washington, DC. The purpose of this ... out research to defend against the threat of bioterrorism ... recognizing that emerging infectious diseases serve as a paradigm ...
... RNA is not merely a transitional state between DNA and ... how genes are turned into a protein product. Gaining a ... on diseases that arise when this function goes awry. ... up to offer a new method for elucidating RNA regulation. ...
... deadly form of primary malignant brain cancer accounting for ... in adults between the ages of 45 and 70. ... only temporarily contained by combined surgery, chemotherapy and radiation ... in an average patient survival time of less than ...
Cached Biology News:Penn biologists establish new method for studying RNA's regulatory 'footprint' 2Penn biologists establish new method for studying RNA's regulatory 'footprint' 3Stopping tumors in their path 2
...
...
... data entry by collecting data from any ... Collect is extremely easy to use ... data entry. An excellent solution for ... spectrophotometers, force gauges, digital calipers, bar code ...
... the autofluorescent pigment lipofuscin accumulates in ... including neurons. The presence of lipofuscin ... fluorescence microscopy in the central nervous ... and emission spectra, which overlaps with ...
Biology Products: