Navigation Links
Monitoring Receptor-Mediated Changes in [Ca2+]i Using Single-Wavelength and Dual-Wavelength Indicators on the FlexStation Workstation


BACKGROUND
CHO-M1 cells contain endogenous nucleotide receptors (P2Y2)3,4 that are linked to activation of phospholipase C, generation of inositol phosphates (IPs), activation of protein kinase C (PKC) and release of intracellular Ca2+ stores5. Purinoceptors are expressed in the vast majority of cells and tissues, where they control a wide range of physiological processes. To date, five subtypes of the P2Y receptor6 have been identified and clonedP2Y1, P2Y2, P2Y4, P2Y6 and P2Y11. The principal physiological ligands for the P2Y2-receptor are UTP and ATP; they show similar potencies7. One important functional role of the P2Y2-receptor is the control of chloride ion fluxes in airway epithelia and mucociliary clearance in the lung, and thus may play a role in cystic fibrosis and other airway disorders.

Novel ligands targeting P2Y-receptors represent potential therapeutic agents and the ability to screen such compounds reliably and reproducibly has been demonstrated using FlexStation8.

Using Z-factor analysis9 and comparison of EC50 estimates and signal magnitude, we show the benefits of ratiometric Ca2+ dyes and homogeneous assay formats over conventional Ca2+ indicators.


METHODS
Cell Preparation
CHO cells (passage number > 40) were routinely grown in 75 cm2 flasks as a monolayer in Hams-F12 medium supplemented with 10% foetal bovine serum and 5 g/mL G418, and maintained at 37˚C in a CO2 incubator. Cells were passaged 1:10 every 2-3 days.

Measurement of [Ca2+]i
CHO cells were seeded into black-walled, clear-base 96-well plates (Costar UK) at
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Versatile Reporter Vectors for Monitoring Viral Transduction
2. Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer
3. Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System
4. Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis
5. Direct Monitoring of Solid-Phase Peptide Synthesis by MALDI-TOF MS
6. Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse
7. New statistical algorithms for Monitoring Gene Expression on GeneChip® Probe Arrays
8. Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon Allelic Discrimination Kits
9. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
10. Oil-Free PCR Using the Hot Top Assembly
11. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
Post Your Comments:
(Date:10/19/2014)... (PRWEB) October 19, 2014 The ... bromine market in Asia-Pacific with analysis and forecast ... the Asia Pacific Bromine Market report, to get ... also provides a glimpse of the segmentation in ... various tables and figures. , http://www.micromarketmonitor.com/market/asia-pacific-bromine-6741503144.html ...
(Date:10/19/2014)... 2014 The Asia-Pacific Speech Analytics market ... Asia-Pacific with analysis and forecast of revenue. This market ... to $208 million by 2019, at a Compound Annual ... , Browse through the TOC of the Asia-Pacific Speech ... in-depth analysis provided. It also provides a glimpse of ...
(Date:10/19/2014)... Asian Orthopedic braces and support systems report defines and segments ... revenue. The Orthopedic braces and support systems market in Asia ... at a developing CAGR of 4.4% from 2013 to 2018. ... and support systems market, to get an idea of the ... segmentation of orthopedic braces and support systems market in the ...
(Date:10/19/2014)... 2014 According to market research ... WLAN Controllers; Wireless gateways)]- Global Advancements, Forecasts and ... global Outdoor Wi-Fi market with analysis and forecasting ... also identifies the drivers and the restrains for ... opportunities and challenges. , Browse 65 market data ...
Breaking Biology Technology:The Asia-Pacific Bromine Market is estimated to grow to $4,080.1 million by 2018 - New Report by MicroMarket Monitor 2The Asia-Pacific Bromine Market is estimated to grow to $4,080.1 million by 2018 - New Report by MicroMarket Monitor 3The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 2The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 3The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 4The Asian Orthopedic braces and support systems market is estimated to grow to around $416.5 million by 2018 - New Report by MicroMarket Monitor 2The Asian Orthopedic braces and support systems market is estimated to grow to around $416.5 million by 2018 - New Report by MicroMarket Monitor 3The Asian Orthopedic braces and support systems market is estimated to grow to around $416.5 million by 2018 - New Report by MicroMarket Monitor 4Outdoor Wi-Fi Market (by Access Points, WLAN Controllers, Wireless gateways) Expected to Cross $37.2 Billion by 2018 - New Report by MarketsandMarkets 2Outdoor Wi-Fi Market (by Access Points, WLAN Controllers, Wireless gateways) Expected to Cross $37.2 Billion by 2018 - New Report by MarketsandMarkets 3Outdoor Wi-Fi Market (by Access Points, WLAN Controllers, Wireless gateways) Expected to Cross $37.2 Billion by 2018 - New Report by MarketsandMarkets 4
... , PRINCETON, N.J. , May 19 ... the Company), a late-stage biopharmaceutical company, announced today that ... patent (US 7,704,985) entitled "Treatment of Irritable Bowel Syndrome ... of beclomethasone dipropionate (BDP) to treat irritable bowel syndrome ...
... from Rensselaer Polytechnic Institute have developed a new nanotechnology-based ... infrared imaging and could lead to a new generation ... leveraging the unique properties of nanoscale gold to "squeeze" ... device, the researchers have doubled the detectivity of a ...
... CHAPEL HILL, N.C. , May 18 ... of educating the marketplace on new medicines. Effectively collaborating ... on treatment options and is especially critical for socially ... Educate the Marketplace " is a 125-page report from ...
Cached Biology Technology:Soligenix Announces Grant of US Patent for the Treatment of Irritable Bowel Syndrome with Oral Beclomethasone Dipropionate 2Soligenix Announces Grant of US Patent for the Treatment of Irritable Bowel Syndrome with Oral Beclomethasone Dipropionate 3Soligenix Announces Grant of US Patent for the Treatment of Irritable Bowel Syndrome with Oral Beclomethasone Dipropionate 4Soligenix Announces Grant of US Patent for the Treatment of Irritable Bowel Syndrome with Oral Beclomethasone Dipropionate 5Soligenix Announces Grant of US Patent for the Treatment of Irritable Bowel Syndrome with Oral Beclomethasone Dipropionate 6Nanotech discovery at Rensselaer could lead to breakthrough in infrared satellite imaging technology 2Nanotech discovery at Rensselaer could lead to breakthrough in infrared satellite imaging technology 3Educating the Marketplace Through Collaborations with Patient Advocacy Groups 2Educating the Marketplace Through Collaborations with Patient Advocacy Groups 3
(Date:10/18/2014)... patients referred for evaluation of suspected genetic conditions, ... 25 percent, including detection of a number of ... disease, according to a study appearing in ... coincide with the American Society of Human Genetics ... or coding regions of thousands of genes simultaneously ...
(Date:10/17/2014)... This news release is available in German . ... for only a very few drugs. When treating overdoses, doctors ... Treatment is especially difficult if there is a combination of ... is playing and accidentally swallows his grandmother,s pills? ETH professor ... Zurich wanted to find an answer to this question. "The ...
(Date:10/16/2014)... cultivated efficiently, they are anything but sustainable: environmental ... cultivation is becoming increasingly evident. Despite their disadvantages, ... are regarded as the sole possibility of achieving ... finds Bernhard Schmid, an ecology professor at the ... of agriculture and forestry. After all, a new ...
Breaking Biology News(10 mins):Whole-exome sequencing shows potential as diagnostic tool 2Emergency aid for overdoses 2Emergency aid for overdoses 3Plant communities produce greater yield than monocultures 2Plant communities produce greater yield than monocultures 3
... the Midwest may have dimmed the memories for some ... landowners, conservationists, crop scientists and soil scientists haven,t forgotten. ... the flood damaged Birds Point-New Madrid floodway in preparation ... "After the waters recede, people forget about the ...
... at Yale School of Medicine have made major discoveries that ... babies. In a mouse study, the team located key molecules ... also found that when parts of these pathways were blocked ... The findings are published online ahead of print ...
... meal is a bi-product following oil extraction from soybean seeds. ... 40% of the nutritional components of the seeds and dependent ... (a monounsaturated omega-9 fatty acid). The study looked ... cancer. Using a variety of soybean lines which were high ...
Cached Biology News:Restoration and recommendations for flood-damaged bottomlands 2Restoration and recommendations for flood-damaged bottomlands 3Researchers spot molecular control switch for preterm lung disorders 2
Yields DNA greater than 50 kb in length from yeast, fungi, Gram neg. and Gram pos. bacteria...
Mouse monoclonal [1Q17] to TGE Virus peplomer ( Abpromise for all tested applications)....
Jak3 Antibody Ship: Hot Store: -20 C...
14-3-3 tau Antibody Shipping Temperature: HOT Storage Temperature: -20C...
Biology Products: